Live, video-rate super-resolution microscopy using structured illumination and rapid GPU-based parallel processing

Microscopy and Microanalysis

Volumn 17, Issue 2, 2011, Pages 191-196

  Lefman, Jonathan ^a,b   Scott, Keana ^a   Stranick, Stephan ^b  

^a NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

^b NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

CUDA; fluorescence microscopy; GPU; real time; structured illumination; super resolution

Indexed keywords

ARTICLE; COMPUTER GRAPHICS; FLUORESCENCE MICROSCOPY; IMAGE PROCESSING; INSTRUMENTATION; METHODOLOGY; MICROSCOPY;

COMPUTER GRAPHICS; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY, FLUORESCENCE; MICROSCOPY, VIDEO;

EID: 79957467441     PISSN: 14319276     EISSN: 14358115     Source Type: Journal    
DOI: 10.1017/S1431927611000158     Document Type: Article

References (11)

1. BETZIG, E., PATTERSON, G. H., SOUGRAT, R., LINDWASSER, O. W., OLENYCH, S., BONIFACINO, J. S., DAVIDSON, M. W., LIPPINCOTT-SCHWARTZ, J. & HESS, H. F. (2006). Imaging intracellular fluorescent proteins at nanometer resolution. Science 313, 1642-1645. (Pubitemid 44414039)
2. BEVERSLUIS, M., BRYANT, G. & STRANICK, S. (2008). Effects of inhomogeneous fields in superresolving structured-illumination microscopy. J Opt Soc Am A 25, 1371-1377.
3. CASTANO DIEZ, D., MUELLER, H. & FRANGAKIS, A. S. (2007). Implementation and performance evaluation of reconstruction algorithms on graphics processors. J Struct Biol 157, 288-295. (Pubitemid 44880803)
4. DYBA, M. & HELL, S. W. (2002). Focal spots of size lambda/23 open up far-field fluorescence microscopy at 33 nm axial resolution. Phys Rev Lett 88, 163901.
5. GUSTAFSSON, M. G. (2000). Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. J Microsc 198, 82-87. (Pubitemid 30316807)
6. KNER, P., CHHUN, B. B., GRIFFIS, E. R., WINOTO, L. & GUSTAFSSON, M. G. (2009). Super-resolution video microscopy of live cells by structured illumination. Nat Methods 6, 339-342.
7. NEIL, M. A., JUSKAITIS, R. & WILSON, T. (1997). Method of obtaining optical sectioning by using structured light in a conventional microscope. Opt Lett 22, 1905-1907. (Pubitemid 127598999)
8. NEIL, M. A., SQUIRE, A., JUSKAITIS, R., BASTIAENS, P. I. & WILSON, T. (2000). Wide-field optically sectioning fluorescence microscopy with laser illumination. J Microsc 197, 1-4. (Pubitemid 30070460)
9. RUST, M. J., BATES, M. & ZHUANG, X. W. (2006). Sub-diffractionlimit imaging by stochastic optical reconstruction microscopy (STORM). Nat Methods 3, 793-795. (Pubitemid 44445825)
10. SCHMEISSER, M., BURKHARD, C., HEISEN, B. C., LUETTICH, M., BUSCHE, B., HAUER, F., KOSKE, T., KNAUBER, K. & STARK, H. (2009). Parallel, distributed and GPU computing technologies in single-particle electron microscopy. Acta Crystallogr D Biol Crystallogr 65, 659-671.
11. SHIMOBABA, T., SATO, Y., MIURA, J., TAKENOUCHI, M. & ITO, T. (2008). Real-time digital holographic microscopy using the graphic processing unit. Opt Express 16, 11776-11781.