Potent and systematic RNAi mediated silencing with single oligonucleotide compounds

RNA

Volumn 17, Issue 6, 2011, Pages 1032-1037

  Lapierre, Jennifer ^a   Salomon, William ^a,b   Cardia, James ^a   Bulock, Karen ^a   Lam, Jessica T ^a   Stanney, William J ^a   Ford, Glenna ^a   Smith Anzures, Bernice ^a   Woolf, Tod ^a   Kamens, Joanne ^a   Khvorova, Anastasia ^a   Samarsky, Dmitry ^a  

^a RXi Pharmaceuticals Corporation   (United States)

^b UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

Ago2; RNAi; siRNA

Indexed keywords

COMPLEMENTARY RNA; MESSENGER RNA; OLIGONUCLEOTIDE; RNA INDUCED SILENCING COMPLEX;

ARTICLE; CONTROLLED STUDY; DIMERIZATION; GENE SILENCING; GENE TARGETING; HUMAN; HUMAN CELL; PRIORITY JOURNAL; RNA INTERFERENCE; RNA STRUCTURE; STRUCTURE ANALYSIS;

CELLS, CULTURED; GENE SILENCING; HELA CELLS; HUMANS; NUCLEIC ACID CONFORMATION; OLIGONUCLEOTIDES; RNA; RNA INTERFERENCE; RNA, MESSENGER; RNA, SMALL INTERFERING; RNA-INDUCED SILENCING COMPLEX;

MAMMALIA;

EID: 79956188277     PISSN: 13558382     EISSN: 14699001     Source Type: Journal    
DOI: 10.1261/rna.2399411     Document Type: Article

References (13)

1. Castanotto D, Rossi JJ. 2009. The promises and pitfalls of RNA-interference-based therapeutics. Nature 457: 426-433.
2. Chu CY, Rana TM. 2008. Potent RNAi by short RNA triggers. RNA 14: 1714-1719.
3. Hossbach M, Gruber J, Osborn M, Weber K, Tuschl T. 2006. Gene silencing with siRNA duplexes composed of target-mRNA-complementary and partially palindromic or partially complementary single-stranded siRNAs. RNA Biol 3: 82-89. (Pubitemid 44444598)
4. Hutvagner G, McLachlan J, Pasquinelli AE, Balint E, Tuschl T, Zamore PD. 2001. A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA. Science 293: 834-838. (Pubitemid 32743968)
5. Lagos-Quintana M, Rauhut R, Lendeckel W, Tuschl T. 2001. Identification of novel genes coding for small expressed RNAs. Science 294: 853-858. (Pubitemid 33032103)
6. Li W, Cha L. 2007. Predicting siRNA efficiency. Cell Mol Life Sci 64: 1785-1792.
7. Martinez J, Patkaniowska A, Urlaub H, Luhrmann R, Tuschl T. 2002. Single-stranded antisense siRNAs guide target RNA cleavage in RNAi. Cell 110: 563-574. (Pubitemid 35247837)
8. Mathews DH, Disney MD, Childs JL, Schroeder SJ, Zuker M, Turner DH. 2004. Incorporating chemical modification constraints into a dynamic programming algorithm for prediction of RNA secondary structure. Proc Natl Acad Sci 101: 7287-7292. (Pubitemid 38638025)
9. Paddison PJ, Cleary M, Silva JM, Chang K, Sheth N, Sachidanandam R, Hannon GJ. 2004. Cloning of short hairpin RNAs for gene knockdown in mammalian cells. Nat Methods 1: 163-167.
10. Patel DJ, Ma JB, Yuan YR, Ye K, Pei Y, Kuryavyi V, Malinina L, Meister G, Tuschl T. 2006. Structural biology of RNA silencing and its functional implications. Cold Spring Harb Symp Quant Biol 71: 81-93.
11. Salomon W, Bulock K, Lapierre J, Pavco P, Woolf T, Kamens J. 2010. Modified dsRNAs that are not processed by Dicer maintain potency and are incorporated into the RISC. Nucleic Acids Res 38: 3771-3779.
12. Siolas D, Lerner C, Burchard J, Ge W, Linsley PS, Paddison PJ, Hannon GJ, Cleary MA. 2005. Synthetic shRNAs as potent RNAi triggers. Nat Biotechnol 23: 227-231. (Pubitemid 40255334)
13. Wang Y, Juranek S, Li H, Sheng G, Tuschl T, Patel DJ. 2008. Structure of an argonaute silencing complex with a seed-containing guide DNA and target RNA duplex. Nature 456: 921-926.