Potent RNAi by short RNA triggers

RNA

Volumn 14, Issue 9, 2008, Pages 1714-1719

  Chu, Chia Ying ^a   Rana, Tariq M ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

Kinetics of RISC; RNAi; siRNA

Indexed keywords

CYCLIN DEPENDENT KINASE 9; DOUBLE STRANDED RNA; MESSENGER RNA; RIBONUCLEOPROTEIN; RNA INDUCED SILENCING COMPLEX; SMALL INTERFERING RNA;

ARTICLE; CANCER CELL CULTURE; FLUORESCENCE ANALYSIS; GENE DELETION; GENE SILENCING; HELA CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; KINETICS; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN FUNCTION; PROTEIN TARGETING; RNA ANALYSIS; RNA CLEAVAGE; RNA DEGRADATION; RNA INTERFERENCE; RNA STRUCTURE; RNA SYNTHESIS; WILD TYPE;

BASE SEQUENCE; HELA CELLS; HUMANS; RNA INTERFERENCE; RNA, DOUBLE-STRANDED; RNA, SMALL INTERFERING; RNA-INDUCED SILENCING COMPLEX;

EID: 50649103008     PISSN: 13558382     EISSN: 14699001     Source Type: Journal    
DOI: 10.1261/rna.1161908     Document Type: Article

References (9)

1. Brown, K.M., Chu, C.Y., and Rana, T.M. 2005. Target accessibility dictates the potency of human RISC. Nat. Struct. Mol. Biol. 12: 469-470.
2. Caplen, N.J., Parrish, S., Imani, F., Fire, A., and Morgan, R.A. 2001. Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. Proc. Natl. Acad. Sci. 98: 9742-9747.
3. Chiu, Y.L. and Rana, T.M. 2002. RNAi in human cells: Basic structural and functional features of small interfering RNA. Mol. Cell 10: 549-561.
4. Chiu, Y.L. and Rana, T.M. 2003. siRNA function in RNAi: A chemical modification analysis. RNA 9: 1034-1048.
5. Chu, C.Y. and Rana, T.M. 2007. Small RNAs: Regulators and guardians of the genome. J. Cell. Physiol. 213: 412-419.
6. Elbashir, S.M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., and Tuschl, T. 2001a. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411: 494 498.
7. Elbashir, S.M., Martinez, J., Patkaniowska, A., Lendeckel, W., and Tuschl, T. 2001b. Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate. EMBO J. 20: 6877-6888.
8. Filipowicz, W., Bhattacharyya, S.N., and Sonenberg, N. 2008. Mechanisms of post-transcriptional regulation by microRNAs: Are the answers in sight? Nat. Rev. Genet. 9: 102-114.
9. Rana, T.M. 2007. Illuminating the silence: Understanding the structure and function of small RNAs. Nat. Rev. Mol. Cell Biol. 8: 23-36.