A modified fluorimetric neutral filter elution method for analyzing radiation-induced double strand break and repair

Analytical Biochemistry

Volumn 414, Issue 2, 2011, Pages 287-293

  Goutham, Venkatesh H ^a   Kamalesh, Mumbrekar D ^a   Guruprasad, Parashiva K ^b   Vadhiraja, Manjunath B ^b   Satyamoorthy, Kapaettu ^a   Rao Bola Satish, Sadashiva ^a  

^a MANIPAL UNIVERSITY   (India)

^b KASTURBA HOSPITAL   (India)

Author keywords

DNA damage and repair; Lymphocyte; Neutral filter elution; PicoGreen

Indexed keywords

CELL CULTURE; COST EFFECTIVENESS; HAZARDS; LYMPHOCYTES; MICROFILTRATION;

DNA DAMAGE AND REPAIR; DNA DOUBLE STRAND BREAKS; DOSE RESPONSE RELATIONSHIPS; DOUBLE STRAND BREAKS; FLUORESCENT DYES; NEUTRAL FILTERS; PICOGREEN; RADIATION-INDUCED;

DNA;

FLUORESCENT DYE;

ARTICLE; CELL COUNT; CELL SURVIVAL; CELL VIABILITY; COMET ASSAY; DNA DAMAGE; DNA DETERMINATION; DNA FRAGMENTATION; DNA REPAIR; DOUBLE STRANDED DNA BREAK; DYE DILUTION CURVE; FLUOROMETRY; HUMAN; HUMAN CELL; ISOTOPE LABELING; LYMPHOCYTE; MICROFILTRATION; NEUTRAL FILTER ELUTION ASSAY; PH; PRIORITY JOURNAL; RADIATION DOSE; RADIOSENSITIVITY; X IRRADIATION;

EID: 79956062291     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.03.033     Document Type: Article

References (26)

1. E.C. Jonathan, E.J. Bernhard, W.G. McKenna, How does radiation kill cells?, Curr Opin. Chem. Biol. 3 (1999) 77-83. (Pubitemid 29118984)
2. M. Wojewódzka, I. Buraczewska, I. Szumiel, I. Gradzka, DNA double-strand break rejoining in radio adapted human lymphocytes: evaluation by neutral comet assay and pulse-field gel electrophoresis, Nukleonika 51 (2006) 185-191. (Pubitemid 44813962)
3. A. Sak, S. Grehl, P. Erichsen, M. Engelhard, A. Grannass, S. Levegrun, C. Pottgen, M. Groneberg, M. Stuschke, Gamma-H2AX foci formation in peripheral blood lymphocytes of tumor patients after local radiotherapy to different sites of the body: dependence on the dose-distribution, irradiated site and time from start of treatment, Int. J. Radiat. Biol. 83 (2007) 639-652.
4. M.O. Bradley, K.W. Kohn, X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elution, Nucleic Acids Res. 7 (1979) 793-804.
5. K. Rothkamm, M. Lobrich, Evidence for a lack of DNA double-strand break repair in human cells exposed to very low x-ray doses, Proc. Natl. Acad. Sci. USA 100 (2003) 5057-5062. (Pubitemid 36542657)
6. A. Muslimovic, I.H. Ismail, Y. Gao, O. Hammarsten, An optimized method for measurement of gamma-H2AX in blood mononuclear and cultured cells, Nat. Protoc. 3 (2008) 1187-1193.
7. P.L. Olive, J.P. Banath, M. Keyes, Residual gammaH2AX after irradiation of human lymphocytes and monocytes in vitro and its relation to late effects after prostate brachytherapy, Radiother. Oncol. 86 (2008) 336-346.
8. P.J. Johnston, S.H. MacPhail, T.D. Stamato, C.U. Kirchgessner, P.L. Olive, Higherorder chromatin structure-dependent repair of DNA double-strand breaks: involvement of the V(D)J recombination double-strand break repair pathway, Radiat. Res. 149 (1998) 455-462.
9. J. Russell, T.E. Wheldon, P. Stanton, A radioresistant variant derived from a human neuroblastoma cell line is less prone to radiation-induced apoptosis, Cancer Res. 55 (1995) 4915-4921.
10. J. Lu, M.R. Kaeck, C. Jiang, G. Garcia, H.J. Thompson, A filter elution assay for the simultaneous detection of DNA double and single strand breaks, Anal. Biochem. 235 (1996) 227-233.
11. P. Leanderson, K. Wennerberg, C. Tagesson, DNA microfiltration assay: a simple technique for detecting DNA damage in mammalian cells, Carcinogenesis 15 (1994) 137-139. (Pubitemid 24121638)
12. T.T. Puck, P.I. Marcus, A rapid method for viable cell titration and clone production with Hela cells in tissue culture: the use of X-irradiated cells to supply conditioning factors, Proc. Natl. Acad. Sci. USA 41 (1955) 432-437.
13. P. Price, T.J. McMillan, Use of the tetrazolium assay in measuring the response of human tumor cells to ionizing radiation, Cancer Res. 50 (1990) 1392-1396. (Pubitemid 20090446)
14. P.J. Johnston, P.E. Bryant, Lack of interference of DNA single-strand breaks with the measurement of double-strand breaks in mammalian cells using the neutral filter elution assay, Nucleic Acids Res. 19 (1991) 2735-2738. (Pubitemid 21896912)
15. V.L. Singer, L.J. Jones, S.T. Yue, R.P. Haugland, Characterization of PicoGreen reagent and development of a fluorescence-based solution assay for doublestranded DNA quantitation, Anal. Biochem. 249 (1997) 228-238.
16. A.I. Dragan, E.S. Bishop, J.R. Casas-Finet, R.J. Strouse, M.A. Schenerman, C.D. Geddes, Metal-enhanced PicoGreen fluorescence: application for doublestranded DNA quantification, Anal. Biochem. 396 (2010) 8-12.
17. R. Batel, Z. Jaksic, N. Bihari, B. Hamer, M. Fafandel, C. Chauvin, H.C. Schroder, W.E. Muller, R.K. Zahn, A microplate assay for DNA damage determination (fast micromethod), Anal. Biochem. 270 (1999) 195-200. (Pubitemid 29252530)
18. B.S. Kaur, E.R. Blazek, Subdenaturing (pH 11.1) filter elution: more sensitive quantification of DNA double-strand breaks, Radiat. Res. 147 (1997) 569-578.
19. M. Shahidi, H. Mozdarani, P.E. Byrant, Radiation sensitivity of leukocytes from healthy individuals and breast cancer patients as measured by the alkaline and neutral comet assay, Cancer Lett. 257 (2007) 263-273. (Pubitemid 47538831)
20. B. Rydberg, Clusters of DNA damage induced by ionising radiation: formation of short DNA fragments. II. Experimental detection, Radiat. Res. 145 (1996) 200-209. (Pubitemid 26066331)
21. P.L. Olive, J.P. Banath, R.E. Durand, The range of oxygenation in SiHa tumor xenografts, Radiat. Res. 158 (2002) 159-166.
22. A. Saxena, C. Yashar, D.D. Taylor, C. Gercel-Taylor, Cellular response to chemotherapy and radiation in cervical cancer, Am. J. Obstet. Gynecol. 192 (2005) 1399-1403.
23. S.J. DiBiase, J. Guan, W.J. Curran Jr., G. Iliakis, Repair of DNA double-strand breaks and radiosensitivity to killing in an isogenic group of p53 mutant cell lines, Int. J. Radiat. Oncol. Biol. Phys. 45 (1999) 743-751. (Pubitemid 29462658)
24. K. Okaichi, L.H. Wang, M. Ihara, Y. Okumura, Sensitivity to ionizing radiation in Saos-2 cells transfected with mutant p53 genes depends on the mutation position, J. Radiat. Res. (Tokyo) 39 (1998) 111-118. (Pubitemid 128461222)
25. E. Dikomey, J. Dahm-Daphi, I. Brammer, R. Martensen, B. Kaina, Correlation between cellular radiosensitivity and non-repaired double-strand breaks studied in nine mammalian cell lines, Int. J. Radiat. Biol. 73 (1998) 269-278. (Pubitemid 28160913)
26. M. Goumenou, K. Machera, Measurement of DNA single strand breaks by alkaline elution and fluorimetric DNA quantification, Anal. Biochem. 326 (2004) 146-152.