Conversion of 7-ketocholesterol to oxysterol metabolites by recombinant CYP27A1 and retinal pigment epithelial cells

Journal of Lipid Research

Volumn 52, Issue 6, 2011, Pages 1117-1127

  Heo, Gun Young ^a   Bederman, Ilya ^a   Mast, Natalia ^a   Liao, Wei Li ^b,d   Turko, Illarion V ^b,c   Pikuleva, Irina A ^a  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^c NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

^d Expression Pathology Inc   (United States)

Author keywords

3 ,27 dihydroxy 5 cholesten 7 one; 3 hydroxy 5 cholesten 7 one 26 oic acid; Age related macular degeneration; Cytochrome P450 27A1; Isotope dilution gas chromatography mass spectrometry

Indexed keywords

3BETA HYDROXY 5 CHOLESTEN 7 ONE 26 OIC ACID; 3BETA,27 DIHYDROXY 5 CHOLESTEN 7 ONE; 7 OXOCHOLESTEROL; CHOLESTANETRIOL 26 MONOOXYGENASE; CHOLESTEROL; OXYSTEROL; RECOMBINANT CHOLESTANETRIOL 26 MONOOXYGENASE; RECOMBINANT ENZYME; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; CHOLESTEROL METABOLISM; ENZYME ACTIVITY; ENZYME SUBSTRATE; HUMAN; HUMAN TISSUE; IN VITRO STUDY; MITOCHONDRIAL RESPIRATION; NONHUMAN; OXIDATION REDUCTION REACTION; PIGMENT EPITHELIUM; PRIORITY JOURNAL; PROTEIN EXPRESSION; RETINA NEUROEPITHELIAL LAYER; TISSUE HOMOGENATE;

ACIDS; ANIMALS; CATTLE; CELL EXTRACTS; CYTOCHROME P-450 CYP27A1; CYTOCHROME P-450 ENZYME SYSTEM; EPITHELIAL CELLS; EYE DISEASES; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HUMANS; KETOCHOLESTEROLS; MITOCHONDRIA; NEURONS; OXIDATION-REDUCTION; RECOMBINANT PROTEINS; RETINAL PIGMENT EPITHELIUM; SUBSTRATE SPECIFICITY;

EID: 79956014823     PISSN: 00222275     EISSN: 15397262     Source Type: Journal    
DOI: 10.1194/jlr.M014217     Document Type: Article

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