Local fluorescent probes for the fluorescence resonance energy transfer scanning near-field optical microscopy

Applied Physics Letters

Volumn 80, Issue 15, 2002, Pages 2625-2627

  Shubeita, G T ^a   Sekatskii, S K ^a   Dietler, G ^a   Letokhov, V S ^b  

^a UNIVERSITY OF LAUSANNE   (Switzerland)

^b INSTITUTE OF SPECTROSCOPY   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

ACCEPTOR MOLECULES; DYE MOLECULE; FIBER TIP; FLUORESCENCE PROBES; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT PROBES; OPTICAL IMAGE; POLYMER LAYERS; SCANNING NEAR-FIELD OPTICAL MICROSCOPY; SELF-SHARPENING; SPATIAL RESOLUTION;

ENERGY TRANSFER; FLUORESCENCE; GEOMETRICAL OPTICS; MOLECULES; OPTICAL DATA STORAGE; PROBES; SCANNING;

NEAR FIELD SCANNING OPTICAL MICROSCOPY;

EID: 79956012603     PISSN: 00036951     EISSN: None     Source Type: Journal    
DOI: 10.1063/1.1467695     Document Type: Article

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19. The following estimation of the overall efficiency can be given. Geometrical factor for the 40× microobjective used having a numerical aperture of 0.65 gives 0.1 for the collection efficiency, then 0.5 is the photomultiplier tube detection efficiency, 0.1: the combined quantum yields of the donor and the acceptor, 0.2: spectral filtering, reflections, and other losses in the optics. All these numbers are real but probably still a bit too optimistic, so we believe that their product is the maximum attainable overall efficiency, whence the estimation given in the text.
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