Cloning and biochemical properties of a highly thermostable and enantioselective nitrilase from Alcaligenes sp. ECU0401 and its potential for (R)-(2)-mandelic acid production

Bioprocess and Biosystems Engineering

Volumn 34, Issue 3, 2011, Pages 315-322

  Zhang, Zhi Jun ^a   Xu, Jian He ^a   He, Yu Cai ^b   Ouyang, Li Ming ^a   Liu, You Yan ^c  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b CHANGZHOU UNIVERSITY   (China)

^c GUANGXI UNIVERSITY   (China)

Author keywords

(R) ( ) mandelic acid; Alcaligenes sp; Biochemical properties; Enantioselective; Nitrilase; Thermostable

Indexed keywords

ALCALIGENES SP; BIOCHEMICAL PROPERTIES; ENANTIOSELECTIVE; MANDELIC ACID; NITRILASE; THERMOSTABLE;

CLONING; ENANTIOSELECTIVITY; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GENES; PURIFICATION;

SIZE EXCLUSION CHROMATOGRAPHY;

EDETIC ACID; MANDELIC ACID; NITRILASE;

ALCALIGENES; ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; DNA SEQUENCE; ENANTIOSELECTIVITY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME METABOLISM; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GENE OVEREXPRESSION; HYDROLYSIS KINETICS; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH MEASUREMENT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; TEMPERATURE SENSITIVITY;

ACETONITRILES; ALCALIGENES; AMINOHYDROLASES; CATIONS, DIVALENT; CLONING, ORGANISM; EDETIC ACID; ENZYME STABILITY; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; MANDELIC ACIDS; RECOMBINANT PROTEINS; STEREOISOMERISM; SUBSTRATE SPECIFICITY; TEMPERATURE;

ALCALIGENES; ALCALIGENES SP.; ESCHERICHIA COLI;

EID: 79955942496     PISSN: 16157591     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00449-010-0473-z     Document Type: Article

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