Quantification of lentiviral vector copy numbers in individual hematopoietic colony-forming cells shows vector dose-dependent effects on the frequency and level of transduction

Gene Therapy

Volumn 18, Issue 5, 2011, Pages 479-487

  Charrier, S ^a,b,c   Ferrand, M ^a,b,c   Zerbato, M ^a,b,c   Precigout G ^a,b,c   Viornery, A ^a,b,c   Bucher Laurent, S ^a   Benkhelifa Ziyyat, S ^a,b,c   Merten, O W ^a   Perea, J ^a,b,c   Galy, A ^a,b,c  

^a GÉNÉTHON   (France)

^b Inserm U951 ^*  (France)

^c UNIVERSITÉ D EVRY VAL D ESSONNE   (France)

Author keywords

CD34+ cell; CFC; hematopoietic cells; Lentivirus; method; Q PCR

Indexed keywords

LENTIVIRUS VECTOR;

ARTICLE; COLONY FORMING UNIT; DOSE RESPONSE; FEASIBILITY STUDY; GENE DOSAGE; GENE EXPRESSION; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; NONHUMAN; PARAMETER; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; VIRAL GENE DELIVERY SYSTEM;

ANTIGENS, CD34; CELL LINE; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; HEMATOPOIETIC STEM CELLS; HUMANS; LENTIVIRUS; STEM CELLS; TRANSDUCTION, GENETIC; VIRUS INTEGRATION;

LENTIVIRUS;

EID: 79955936790     PISSN: 09697128     EISSN: 14765462     Source Type: Journal    
DOI: 10.1038/gt.2010.163     Document Type: Article

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