Multiple aggregates and aggresomes of C-terminal truncated human αA-crystallins in mammalian cells and protection by αB-crystallin

PLoS ONE

Volumn 6, Issue 5, 2011, Pages

  Raju, Ilangovan ^a   Kumarasamy, Anbarasu ^b   Abraham, Edathara C ^a  

^a UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES   (United States)

^b BHARATHIDASAN UNIVERSITY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA CRYSTALLIN; GAMMA TUBULIN; CRYSTALLIN;

AGGRESOME; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL PROTECTION; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HELA CELL; IMMUNOCYTOCHEMISTRY; MAMMAL CELL; PROTEIN AGGREGATION; PROTEIN CLEAVAGE; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; SEQUENCE TAGGED SITE; WESTERN BLOTTING; CHEMISTRY; CONFOCAL MICROSCOPY; FLUORESCENCE MICROSCOPY; HUMAN; METABOLISM;

MAMMALIA;

BLOTTING, WESTERN; CRYSTALLINS; FLUORESCENCE RESONANCE ENERGY TRANSFER; HELA CELLS; HUMANS; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE;

EID: 79955936253     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0019876     Document Type: Article

References (34)

1. Van Der Ouderra FJ, De Jong WW, Bloemendal H, (1973) The amino- acid sequence of the alphaA2 chain of bovine alpha-crystallin. Eur J Biochem 39: 207-222.
2. Van Der Ouderra FJ, De Jong WW, Hilderink A, Bloemendal H, (1974) The amino-acid sequence of the alphaB2 chain of bovine alpha-crystallin. Eur J Biochem 49: 157-168.
3. De Jong WW, Terwindt EC, Bloemendal H, (1975) The amino-acid sequence of the A chain of human alpha-crystallin. FEBS Lett 58: 310-313.
4. Ingolia TD, Craig EA, (1982) Four small Drosophila heat shock proteins are related to each other and to mammalian α-crystallin. Proc Natl Acad Sci USA 79: 2360-2364.
5. Horwitz J, (1992) α-Crystallin can function as a molecular chaperone. Proc Natl Acad Sci USA 89: 10449-10453.
6. Wang K, Spector A, (1994) The chaperone activity of bovine alpha-crystallin. Interaction with other lens crystallins in native and denatured states. J Biol Chem 269: 13601-13608.
7. Rao PV, Huang QL, Horwitz J, Zigler JS, (1995) Evidence that alpha-crystallin prevents non-specific protein aggregation in the intact eye lens. Biochim Biophys Acta 1245: 439-444.
8. Thampi P, Abraham EC, (2003) Influence of the C-terminal residues on oligomerization of αA-crystallin. Biochemistry 42: 11857-11863.
9. Rajan S, Chandrashekar R, Aziz A, Abraham EC, (2006) Role of arginine-163 and the 163-REEK-166 motif in the oligomerization of truncated αA-crystallins. Biochemistry 45: 15684-15691.
10. Takemoto LJ, (1995) Identification of the in vivo truncation sites at the C-terminal region of alpha-A-crystallin from aged bovine and human lens. Curr Eye Res 14: 837-841.
11. Lund AL, Smith JB, Smith DL, (1996) Modifications of the water-insoluble human lens alpha-crystallins. Exp Eye Res 63: 661-672.
12. Colvis C, Garland D, (2002) Posttranslational modification of human αA-crystallin: correlation with electrophoretic migration. Arch Biochem Biophys 397: 319-323.
13. Ma Z, Hanson SR, Lampi KJ, David LL, Smith DL, et al. (1998) Age-related changes in human lens crystallins identified by HPLC and mass spectrometry. Exp Eye Res 67: 21-30.
14. Miesbauer LR, Zhou X, Yang Z, Sun Y, Smith DL, et al. (1994) Posttranslational modifications of water-soluble human lens crystallins from young adults. J Biol Chem 269: 12494-12502.
15. Takemoto LJ, (1998) Quantitation of specific cleavage sites at the C-terminal region of alpha-A-crystallin from human lenses of different age. Exp Eye Res 66: 263-266.
16. Thampi P, Hassan A, Smith JB, Abraham EC, (2002) Enhanced C-terminal truncation of alphaA- and alphaB-crystallins in diabetic lenses. Invest Ophthalmol Vis Sci 43: 3265-3272.
17. Aziz A, Santhoshkumar P, Sharma KK, Abraham EC, (2007) Cleavage of the C-terminal serine of human αA-crystallin produces αA1-172 with increased chaperone activity and oligomeric size. Biochemistry 46: 2510-2519.
18. Kallur LS, Aziz A, Abraham EC, (2008) C-Terminal truncation affects subunit exchange of human αA-crystallin with αB-crystallin. Mol Cell Biochem 308: 85-91.
19. Kopito RR, (2000) Aggresomes, inclusion bodies and protein aggregation. Trends Cell Biol 10: 524-530.
20. Garcia-Mata R, Gao YS, Sztul E, (2002) Hassles with taking out the garbage: aggravating aggresomes. Traffic 3: 388-396.
21. Johnston JA, Ward CL, Kopito RR, (1998) Aggresomes: a cellular response to misfolded proteins. J Cell Biol 143: 1883-1898.
22. Arrasate M, Mitra S, Schweitzer ES, Segal MR, Finkbeiner S, (2004) Inclusion body formation reduces levels of mutant huntingin and the risk of neuronal death. Nature 431: 805-810.
23. Shen D, Coleman J, Chan E, Nicholson TP, Dai L, et al. (2010) Novel Cell- and Tissue-based Assays for Detecting Misfolded and Aggregated Protein Accumulation within Aggresomes and Inclusion Bodies. Cell Biochem Biophys (In Press).
24. Junn E, Lee SS, Suhr UT, Mouradian MM, (2002) Parkin Accumulation in Aggresomes Due to Proteasome Impairment. J Biol Chem 277: 47870-47877.
25. Jones RJ, Jourd'heuil D, Salerno JC, Smith MES, Singer HA, (2007) iNos regulation by calcium/calmodulin-dependent protein kinase II in vascular smooth muscle. Am J Physiol Heart Circ Physiol 292: H2634-H2642.
26. Kaneko M, Koike H, Saito R, Kitamura Y, Okuma Y, et al. (2010) Loss of HRD-1 Mediated Protein Degradation Causes Amyloid Precursor Protein Accumulation and Amyloid-β Generation. J Neurosci 30: 3924-3932.
27. Mukit MKM, Davidson SM, Smith PMD, MacCormac LP, Kahns S, et al. (2004) Parkin is recruited into aggresomes in a stress-specific manner: over-expression of parkin reduces aggresome formation but can be dissociated from parkin's neuronal survival. Hum Mol Genet 13: 117-135.
28. Heir R, Ablasou C, Dumontier E, Elliot M, Fagotto-Kaufmann C, et al. (2006) The UBL domain of PLIC-1 regulates aggresome formation. EMBO Rep 7: 1252-1258.
29. Iwata A, Riley BE, Johnston JA, Kopito RR, (2005) HDAC6 and microtubules are required for autophagic degradation of aggregated huntingtin. J Biol Chem 280: 40282-40292.
30. Ma A, Lindquist S, (2001) Wild-type PrP and mutant associated with prion disease are subject to retrograde transport and proteasome degradation. Proc Natl Acad Sci U S A 98: 14955-14960.
31. Sha Y, Pandit L, Zeng S, Eissa NT, (2009) A critical role for CHIP in the aggresome pathway. Mol Cell Biol 29: 116-128.
32. Chavez Zobel AT, Loranger A, Marceau N, Theriault JR, Lambert H, et al. (2003) Distinct chaperone mechanisms can delay the formation of aggresomes by the myopathy-causing R120G alphaB-crystallin mutant. Hum Mol Genet 12: 1609-1620.
33. Zaarur N, Merrin AB, Gabai VL, Sherman MY, (2008) Triggering aggresome formation. Dissecting aggresome-targeting and aggregation signals in synphilin 1. J Biol Chem 283: 25575-25584.
34. Taylor JP, Tanaka F, Robitschek J, Sandoval JM, Taye A, et al. (2003) Aggresomes protects cells by enhancing the degradation of toxic-polyglutamine containing protein. Hum Mol Genet 12: 749-757.