Novel Cell- and Tissue-Based Assays for Detecting Misfolded and Aggregated Protein Accumulation Within Aggresomes and Inclusion Bodies

Cell Biochemistry and Biophysics

Volumn 60, Issue 3, 2011, Pages 173-185

  Shen, Dee ^a   Coleman, Jack ^a   Chan, Eric ^a   Nicholson, Thomas P ^a   Dai, Lijun ^a   Sheppard, Paul W ^a   Patton, Wayne F ^a  

^a Enzo Life Sciences Inc   (United States)

Author keywords

Aggresome; Alzheimer's disease; Autophagy; Inclusion bodies; LC3; Misfolded proteins; p62 protein; Proteasome inhibitor; Protein homeostasis; Proteostasis; Ubiquitin proteasome system

Indexed keywords

BENZYLOXYCARBONYLLEUCYL LEUCYL LEUCINE ALDEHYDE; BENZYLOXYCARBONYLLEUCYL-LEUCYL-LEUCINE ALDEHYDE; FLUORESCENT DYE; LEUPEPTIN; PROTEASOME; PROTEIN; THIAZOLE DERIVATIVE; THIOFLAVINE;

ARTICLE; AUTOPHAGY; CELL INCLUSION; CHEMISTRY; DRUG ANTAGONISM; FLUORESCENT ANTIBODY TECHNIQUE; HUMAN; METABOLISM; METHODOLOGY; PROTEIN FOLDING; TUMOR CELL LINE; UBIQUITINATION;

AUTOPHAGY; CELL LINE, TUMOR; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; FLUORESCENT DYES; HUMANS; INCLUSION BODIES; LEUPEPTINS; PROTEASOME ENDOPEPTIDASE COMPLEX; PROTEIN FOLDING; PROTEINS; THIAZOLES; UBIQUITINATION;

EID: 79958290302     PISSN: 10859195     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12013-010-9138-4     Document Type: Article

References (24)

1. Kopito, R. R. (2000). Aggresomes, inclusion bodies and protein aggregation. Trends in Cell Biology, 10, 524-530.
2. Amijee, H., Madine, J., Middleton, D. A., & Doig, A. J. (2009). Inhibitors of protein aggregation and toxicity. Biochemical Society Transactions, 37(4), 692-696.
3. Dedeoglu, A., Kubilus, J. K., Jeitner, T. M., Matson, S. A., Bogdanov, M., Kowall, N. W., et al. (2002). Therapeutic effects of cystamine in a murine model of Huntington's disease. The Journal of Neuroscience, 22(20), 8942-8950.
4. Taylor, J. P., Tanaka, F., Robitschek, J., Sandoval, C. M., Taye, A., Markovic-Plese, S., et al. (2003). Aggresomes protect cells by enhancing the degradation of toxic polyglutamine-containing protein. Human Molecular Genetics, 12, 749-757.
5. Zaarur, N., Meriin, A. B., Gabai, V. L., & Sherman, M. Y. (2008). Triggering aggresome formation. Dissecting aggresome-targeting and aggregation signals in synphilin 1. Journal of Biological Chemistry, 283(41), 27575-27584.
6. Beaudoin, S., Goggin, K., Bissonnette, C., Grenier, C., & Roucou, X. (2008). Aggresomes do not represent a general cellular response to protein misfolding in mammalian cells. BMC Cell Biology, 9, 59. doi: 10. 1186/1471-2121-9-59.
7. Saito, Y., Kishida, K., Takata, K., Takahashi, H., Shimada, T., Tanaka, K., et al. (2009). A green fluorescent protein fused to rice prolamin forms protein body-like structures in transgenic rice. Journal of Experimental Botany, 60(2), 615-627.
8. Hollo, Z., Homolya, L., Davis, C. W., & Sarkadi, B. (1994). Calcein accumulation as a fluorometric functional assay of the multidrug transporter. Biochimica et Biophysica Acta, 1191(2), 384-388.
9. Mu, T. W., Ong, D. S., Wang, Y. J., Balch, W. E., Yates, J. R, 3rd, Segatori, L., et al. (2008). Proteostasis regulators and pharmacologic chaperones synergise to correct protein misfolding diseases. Cell, 134(5), 769-781.
10. Murakawa, Y., Sonoda, E., Barber, L. J., Zeng, W., Yokomori, K., Kimura, H., et al. (2007). Inhibitors of the proteasome suppress homologous DNA recombination in mammalian cells. Cancer Research, 67(18), 8536-8543.
11. Kawaguchi, Y., Kovacs, J. J., McLaurin, A., Vance, J. M., Ito, A., & Yao, T. P. (2003). The deacetylase HDAC6 regulates aggresome formation and cell viability in response to misfolded protein stress. Cell, 115, 727-738.
12. Nawrocki, S. T., Carew, J. S., Pino, M. S., Highshaw, R. A., Andtbacka, R. H., Dunner, K, Jr, et al. (2006). Aggresome disruption: a novel strategy to enhance bortezomib-induced apoptosis in pancreatic cancer cells. Cancer Research, 66, 3773-3781.
13. Sarkar, S., Perlstein, E. O., Imarisio, S., Pineau, S., Cordenier, A., Maglathlin, R. L., et al. (2007). Small molecules enhance autophagy and reduce toxicity in Huntington's disease models. Nature Chemical Biology, 3(6), 331-338.
14. Renna, M., Jimenez-Sanchez, M., Sarkar, S., & Rubinsztein, D. C. (2010). Chemical inducers of autophagy that enhance the clearance of mutant proteins in neurodegenerative diseases. Journal of Biological Chemistry, 285(15), 11061-11067.
15. Westermark, G. T., Johnson, K. H., Westermark, P. (1999). Staining methods for identification of amyloid in tissue. Methods in Enzymology, 309, 3-25.
16. Biancalana, M., & Koide, S. (2010). Molecular mechanism of Thioflavin-T binding to amyloid fibrils. Biochimica et Biophysica Acta, 1804(7), 1405-1412.
17. Hudson, S. A., Ecroyd, H., Kee, T. W., & Carver, J. A. (2009). The Thioflavin T fluorescence assay for amyloid fibril detection can be biased by the presence of exogenous compounds. FEBS Journal, 276, 5960-5972.
18. Viegas, M. S., Martins, T. C., Seco, F., & do Carmo, A. (2007). An improved and cost-effective methodology for the reduction of autofluorescence in direct immunofluorescence studies on formalin-fixed paraffin-embedded tissues. European Journal of Histochemistry, 51(1), 59-66.
19. Ando, Y., Haraoka, K., Terazaki, H., Tanoue, Y., Ishikawa, K., Katsuragi, S., et al. (2003). Novel tool for detecting amyloid deposits in systemic amyloidosis in vitro and in vivo. Laboratory Investigation, 83(12), 1751-1759.
20. Wang, Y. P., Biernat, J., Pickhardt, M., Mandelkow, E., & Mandelkow, E. M. (2007). Stepwise proteolysis liberates tau fragments that nucleate the Alzheimer-like aggregation of full-length tau in a neuronal cell model. Proceedings of the National Academy of Science, 104(24), 10252-10257.
21. Bennett, E. J., Bence, N. F., Jayakumar, R., & Kopito, R. R. (2005). Global impairment of the ubiquitin-proteasome system by nuclear or cytoplasmic protein aggregates precedes inclusion body formation. Molecular Cell, 17(3), 351-365.
22. Ross C. A., Poirier M. A. (2004). Protein aggregation and neurodegenerative disease. Nature Medicine, S10-S17.
23. Bjørkøy, G., Lamark, T., Brech, A., Outzen, H., Perander, M., Overvatn, A., et al. (2005). p62/SQSTM1 forms protein aggregates degraded by autophagy and has a protective effect on huntingtin-induced cell death. Journal of Cell Biology, 171(4), 603-614.
24. Crowe, A., Huang, W., Ballatore, C., Johnson, R. L., Hogan, A. M., Huang, R., et al. (2009). Identification of aminothienopyridazine inhibitors of tau assembly by quantitative high-throughput screening. Biochemistry, 48(32), 7732-7745.