Design and efficient production of bovine enterokinase light chain with higher specificity in E. coli

Biotechnology Letters

Volumn 33, Issue 6, 2011, Pages 1227-1232

  Chun, Haarin ^a   Joo, Keehyoung ^b   Lee, Jooyoung ^b   Shin, Hang Cheol ^a  

^a Soongsil University   (South Korea)

^b Korea Institute for Advanced Study   (South Korea)

Author keywords

Enterokinase; Fusion protein; Mutants; Protein disulfide isomerase

Indexed keywords

C-TERMINAL PEPTIDES; E. COLI; EFFICIENT PRODUCTION; ENTEROKINASE; FUSION PROTEINS; FUSION SYSTEMS; LIGHT CHAIN; LYSINE RESIDUES; MUTANTS; PROTEIN DISULFIDE ISOMERASE; PROTEIN DISULFIDE ISOMERASES; RECOGNITION SEQUENCE; RECOMBINANT PROTEIN; SERINE PROTEASE; SITE-SPECIFIC;

AMINO ACIDS; ENZYMES; ESCHERICHIA COLI;

PROTEINS;

ENTEROPEPTIDASE; HYBRID PROTEIN; OLIGOPEPTIDE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMAL; ARTICLE; BIOTECHNOLOGY; CATTLE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENETICS; IN VITRO STUDY; KINETICS; METABOLISM; PROTEIN ENGINEERING; PROTEIN QUATERNARY STRUCTURE; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; BIOTECHNOLOGY; CATTLE; ENTEROPEPTIDASE; ESCHERICHIA COLI; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; OLIGOPEPTIDES; PROTEIN ENGINEERING; PROTEIN STRUCTURE, QUATERNARY; RECOMBINANT FUSION PROTEINS; SUBSTRATE SPECIFICITY;

BOVINAE; ESCHERICHIA COLI;

EID: 79955935196     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-011-0562-3     Document Type: Article

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