Production of a recombinant bovine enterokinase catalytic subunit in the methylotrophic yeast Pichia pastoris

Bio/Technology

Volumn 14, Issue 1, 1996, Pages 77-81

  Vozza, Laura A ^a   Wittwer, Leonard ^a   Higgins, David R ^a   Purcell, Thomas J ^a   Bergseid, Mark ^a   Collins Racie, Lisa A ^b   Lavallie, Edward R ^b   Hoeffler, James P ^a  

^a INVITROGEN CORPORATION   (United States)

^b PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; ENTEROPEPTIDASE; HYBRID PROTEIN; POLYHISTIDINE; PROTEINASE; RECOMBINANT PROTEIN; THIOREDOXIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CATTLE; ENZYME PURIFICATION; ENZYME SUBSTRATE; ENZYME SUBUNIT; FUNGAL GENETICS; GENE EXPRESSION; GENETIC ENGINEERING; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR CLONING; NONHUMAN; YEAST; ENZYME ACTIVE SITE; PICHIA PASTORIS;

ANIMALS; ARTIFICIAL GENE FUSION; BINDING SITES; CATALYSIS; CATTLE; ENTEROPEPTIDASE; ESCHERICHIA COLI; GENETIC VECTORS; HISTIDINE; PEPTIDE FRAGMENTS; PEPTIDES; PICHIA; RECOMBINANT PROTEINS; SOLUBILITY; THIOREDOXIN;

BOS TAURUS; BOVINAE; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 0030040517     PISSN: 0733222X     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0196-77     Document Type: Article

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