Functional expression and purification of bovine enterokinase light chain in recombinant Escherichia coli

Preparative Biochemistry and Biotechnology

Volumn 37, Issue 3, 2007, Pages 205-217

  Huang, Lei ^a   Ruan, Hong ^b   Gu, Weiyan ^a   Xu, Zhinan ^a   Cen, Peilin ^a   Fan, Limei ^b  

^a ZHEJIANG UNIVERSITY   (China)

^b ZHEJIANG UNIVERSITY CITY COLLEGE   (China)

Author keywords

Bovine enterokinase light chain; Enterokinase; Enzyme activity; Escherichia coli; Fusion expression; Purification

Indexed keywords

ENTEROPEPTIDASE; HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; HYBRID PROTEIN; ISOPROPYL THIOGALACTOSIDE; OLIGOPEPTIDE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; BACTERIUM TRANSFORMATION; BIOSYNTHESIS; CHEMISTRY; CYTOPLASM; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE EXPRESSION; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; PROTEIN ENGINEERING; PROTEIN FOLDING;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ENTEROPEPTIDASE; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; HISTIDINE; ISOPROPYL THIOGALACTOSIDE; OLIGOPEPTIDES; PERIPLASM; PROTEIN ENGINEERING; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; TRANSFORMATION, BACTERIAL;

BOVINAE; ESCHERICHIA COLI;

EID: 34249089817     PISSN: 10826068     EISSN: 15322297     Source Type: Journal    
DOI: 10.1080/10826060701386695     Document Type: Article

References (22)

1. Maroux, S.; Baratti, J.; Desnuelle, P. Purification and specificity of porcine enterokinase. J. Biol. Chem. 1971, 246, 5031-5039.
2. Baratti, J.; Maroux, S.; Louvard, D.; Desnuelle, P. On porcine enterokinase. Further purification and some molecular properties. Biochim. Biophys. Acta 1973, 315, 147-161.
3. Anderson, L.E.; Walsh, K.A.; Neurath, H. Bovine enterokinase. Purification, specificity, and some molecular properties. Biochemistry 1977, 16, 3354-3360.
4. Liepnieks, J.J.; Lighe, A. The preparation and properties of bovine enterokinase. J. Biol. Chem. 1979, 254, 1677-1683.
5. Fonseca, P.; Light, A. The purification and characterization of bovine enterokinase from membrane fragment in the duodenal mucosal fluid. J. Biol. Chem. 1983, 258, 3069-3074.
6. Magee, A.I.; Grant, D.A.; Taylor, J.H. Further studies on the subunit structure and oligosaccharide moiety of human enterokinase. Clin. Chim. Acta 1981, 115, 241-254.
7. Lu, D.; Futterer, K.; Korolev, S.; Zheng, X.; Tan, K.; Waksman, G.; Sadler, J.E. Crystal structure of enteropeptidase light chain complexed with an anolog of the trypsinogen activation peptide. J. Mol. Biol. 1999, 292, 361-373.
8. LaVallie, E.R.; Rehemtulla, A.; Racie, L.A.; DiBlasio, E.A.; Fereny, C.; Grant, K.L.; Light, A.; McCoy, J.M. Cloning and functional expression of a cDNA encoding the catalytic subunit of bovine enterokinase. J. Biol. Chem. 1993, 268, 23311-23317.
9. Collins, L.A.; McColgan, J.M.; Grant, K.L.; DiBlasio, E.A.; McCoy, J.M.; LaVallie, E.R. Production of a recombinant bovine enterokinase catalytic submit in Escherichia coli using the novel secretory fusion partner DsbA. Bio/Technology 1995, 13, 982-987.
10. Yuan, L.D.; Hua, Z.C. Expression, purification, and characterization of a biologically active bovine enterokinase catalytic subunit in Escherichia coli. Protein Exper. Purif. 2002, 25, 300-304.
11. Vozza, L.A.; Wittwer, L.; Higgins, D.R.; Purcell, T.J.; Bergseid, M.; Collins, L.A.; LaVallie, E.R.; Hoeffler, J.P. Production of a recombinant bovine enterokinase catalytic subunit in the methylotropic yeast Pichia pastoris. Biotechnology 1996, 14, 77-81.
12. Peng, L.; Zhong, X.; Ou, J.; Zheng, S.; Liao, J.; Wang, L.; Xu, A. High-level secretory production of recombinant bovine enterokinase light chain by Pichia pastoris. J. Biotechnol. 2004, 108, 185-192.
13. Svetina, M.; Krasevec, N.; Gaberc, V.; Komel, R. Expression of catalytic subunit of bovine enterokinase in the filamentous fungus Aspergillus niger. J. Biotechnol. 2000, 76, 245-251.
14. Choi, S.I.; Song, H.W.; Moon, J.W.; Seong, B.L. Recombinant enterokinase light chain with affinity tag, expression from Saccharomyces cerevisiae and its utilities in fusion protein technology. Biotechnol. Bioeng. 2001, 75, 718-724.
15. Kane, J.F. Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli. Curr. Opin. Biotechnol. 1995, 6, 494-500.
16. Peng, L.; Xu, Z.N.; Fang, X.M.; Wang, F.; Yang, S.; Cen, P.L. Preferential Codons Enhancing the Expression Level of Human Beta-Defensin-2. Protein Peptide Lett. 2004, 11, 339-344.
17. Xu, Z.N.; Zhong, Z.X.; Huang, L.; Peng, L.; Wang, F.; Cen, P.L. High-level production of bioactive human beta-defensin-4 in Escherichia coli by soluble fusion expression. Appl. Microbiol. Biotechnol. 2006, 72, 471-479.
18. Hu, X.Y.; Shi, Q.W.; Yang, T.; Jackowski, G. Specific replacement of consecutive AGG codons results in high-level expression of human cardiac troponin T in Escherichia coli. Protein Express. Purif. 1996, 7, 289-293.
19. Arg. Nucleic Acids Res. 1990, 18, 5031-5036.
20. Lu, D.; Yuan, X.; Zheng, X.; Sadler, J.E. Bovine proenteropeptidase is activated by trypsin, and the specificity of enteropeptidase depends on the heavy chain. J. Biol. Chem. 1997, 272, 31293-31300.
21. Song, H.; Choi, S.; Seong, B.L. Engineered recombinant enteropeptidase catalytic subunit, effect of N-terminal modification. Arch. Biochem. Biophys. 2002, 400, 1-6.
22. Martin, J.L.; Bardwell, J.C.A.; Kuriyan, J. Crystal structure of the DsbA protein required for disulfide bond formation in vivo. Nature 1993, 365, 64-468.