S-selective hydroxynitrile lyase from a plant Baliospermum montanum: Molecular characterization of recombinant enzyme

Journal of Biotechnology

Volumn 153, Issue 3-4, 2011, Pages 100-110

  Dadashipour, Mohammad ^a   Yamazaki, Mizue ^a   Momonoi, Kazumi ^b   Tamura, Ken'ichirou ^a   Fuhshuku, Ken Ichi ^a   Kanase, Yurina ^a   Uchimura, Etsuzoh ^c   Kaiyun, Guan ^d   Asano, Yasuhisa ^a  

^a TOYAMA PREFECTURAL UNIVERSITY   (Japan)

^b Toyama Agricultural Research Center   (Japan)

^c Botanic Gardens of Toyama   (Japan)

^d KUNMING INSTITUTE OF BOTANY   (China)

Author keywords

Alanine scanning; Characterization; Homology modeling; Molecular cloning; S selective hydroxynitrile lyase; Substrate specificity

Indexed keywords

ALANINE SCANNING; HOMOLOGY MODELING; HYDROXYNITRILE LYASES; MOLECULAR CLONING; SUBSTRATE SPECIFICITY;

ALDEHYDES; AMINO ACIDS; AMMONIUM COMPOUNDS; CLONING; ENZYMES; ESCHERICHIA COLI; GENES; KETONES; ORGANIC COMPOUNDS; PHENOLS;

SUBSTRATES;

2 BUTANONE; ALDEHYDE; AMMONIUM SULFATE; BENZYLSULFONYL FLUORIDE; BUFFER; COMPLEMENTARY DNA; CYANOHYDRIN; GENOMIC DNA; HYDROXYNITRILE LYASE; KETONE; PHENOL; RECOMBINANT ENZYME; SILVER NITRATE;

ARTICLE; BALIOSPERMUM MONTANUM; CARBOXY TERMINAL SEQUENCE; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; ENZYME SPECIFICITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; GEL FILTRATION; GENE EXPRESSION; GENE SEQUENCE; INTRON; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; PLANT; PLANT LEAF; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION;

ALDEHYDE-LYASES; BENZALDEHYDES; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; EUPHORBIACEAE; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; NITRILES; PLANT PROTEINS; RECOMBINANT PROTEINS; STEREOISOMERISM; SUBSTRATE SPECIFICITY; TEMPERATURE;

BALIOSPERMUM MONTANUM; ESCHERICHIA COLI;

EID: 79955781277     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.02.004     Document Type: Article

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