Structure determinants of substrate specificity of hydroxynitrile lyase from Manihot esculenta

Protein Science

Volumn 11, Issue 1, 2002, Pages 65-71

  Lauble, Hanspeter ^a   Miehlich, Burkhard ^a   Förster, Siegfried ^a   Kobler, Christoph ^a   Wajant, Harald ^a   Effenberger, Franz ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Active site tunnel mutant; Crystal structure; Substrate specificity

Indexed keywords

ALANINE; HYDROXYNITRILE LYASE; NITRILE; TRYPTOPHAN;

AMINO ACID SUBSTITUTION; ARTICLE; CASSAVA; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME SPECIFICITY; KINETICS; NONHUMAN; PLANT; PRIORITY JOURNAL; X RAY ANALYSIS;

ALDEHYDE-LYASES; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; HYDROGEN; KINETICS; MANIHOT; MODELS, CHEMICAL; MODELS, MOLECULAR; MUTATION; PROTEIN BINDING; TRYPTOPHAN;

BACTERIA (MICROORGANISMS); MANIHOT; MANIHOT ESCULENTA;

EID: 0036136695     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.ps.33702     Document Type: Article

References (20)

1. Free R value: A novel statistical quantity for assessing the accuracy of crystal structures
2. The CCP4 suite: Programs for protein crystallography
3. The first recombinant hydroxynitrile lyase and its application in the synthesis of (S)-cyanohydrins
4. Study of the (S)-hydroxynitrile lyase from Hevea brasiliensis: Mechanistic implications
5. PROCHECK: A program to check the stereochemical quality of protein structures
6. Crystallization and preliminary X-ray diffraction analysis of hydroxynitrile lyase from cassava (Manihot esculenta)
7. Crystal structure of hydroxynitrile lyase from Manihot esculenta in complex with substrates acetone and chloroacetone: Implications for the mechanism of cyanogenesis
8. Mechanistic aspects of cyanogenesis from active-site mutant Ser80Ala of hydroxynitrile lyase from Manihot esculenta in complex with acetone cyanohydrin
9. XtalView/Xfit-A versatile program for manipulating coordinates and electron density
10. The metabolism of aromatic compounds in higher plants. VIII. The requirement of hydroxynitrile lyase for flavine
11. A colorimetric assay for α-hydroxynitrile lyase
12. Mechanism of cyanogenesis: The crystal structure of hydroxynitrile lyase from Hevea brasiliensis
13. Hydroxynitrile lyases of higher plants
14. Identification of potential active-site residues in the hydroxynitrile lyase from Manihot esculenta by site-directed mutagenesis
15. Enantioselective synthesis of aliphatic (S)-cyanohydrins in organic solvents using hydroxynitrile lyase from Manihot esculenta
16. Three-dimensional structures of enzyme-substrate complexes of the hydroxynitrile lyase from Hevea brasiliensis