Expression of β-1,4-galactosyltransferase and suppression of β-N-acetylglucosaminidase to aid synthesis of complex N-glycans in insect Drosophila S2 cells

Journal of Biotechnology

Volumn 153, Issue 3-4, 2011, Pages 145-152

  Kim, Yeon Kyu ^a   Kim, Kyoung Ro ^a   Kang, Dong Gyun ^a   Jang, So Young ^b   Kim, Young Hwan ^b   Cha, Hyung Joon ^a  

^a Pohang University of Science and Technology (POSTECH)   (South Korea)

^b Korea Basic Science Institute (KBSI)   (South Korea)

Author keywords

1,4 Galactosyltransferase; N Acetylglucosaminidase; Drosophila S2 cells; Human erythropoietin; N Glycosylation

Indexed keywords

DROSOPHILA S2 CELLS; GLYCOSYLTRANSFERASES; HUMAN ERYTHROPOIETIN; N-ACETYLGLUCOSAMINE; N-ACETYLGLUCOSAMINIDASE; N-GLYCAN; N-GLYCAN STRUCTURES; N-GLYCANS; N-GLYCOSYLATION; SIALYLATION; SYNERGISTIC EFFECT;

ESTERIFICATION;

GLYCOSYLATION;

BETA N ACETYLGLUCOSAMINYLGLYCOPEPTIDE BETA 1,4 GALACTOSYLTRANSFERASE; ERYTHROPOIETIN; GLYCOPEPTIDASE; GLYCOPROTEIN; N ACETYL BETA GLUCOSAMINIDASE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DROSOPHILA; ENZYME REPRESSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; SIALYLATION;

ACETYLGLUCOSAMINIDASE; ANIMALS; BLOTTING, WESTERN; CELL LINE; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETINAE; CRICETULUS; DROSOPHILA MELANOGASTER; ERYTHROPOIETIN; HUMANS; MICROSCOPY, FLUORESCENCE; N-ACETYLLACTOSAMINE SYNTHASE; POLYSACCHARIDES; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; RNA INTERFERENCE;

HEXAPODA;

EID: 79955773528     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.03.021     Document Type: Article

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