Novel insect cell line capable of complex N-glycosylation and sialylation of recombinant proteins

Biotechnology Progress

Volumn 19, Issue 1, 2003, Pages 185-192

  Palomares, Laura A ^c   Joosten, Christoph E ^a   Hughes, Patrick R ^b   Granados, Robert R ^b   Shuler, Michael L ^a  

^a Department of Obstetrics Gynecology   (United States)

^b United States Department of Agriculture Agricultural Research Service and Boyce Thompson Institute for Plant Research   (United States)

^c INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; CONCENTRATION (PROCESS); PRODUCTIVITY; PROTEINS;

GLYCOSYLATION; RECOMBINANT PROTEINS;

BIOTECHNOLOGY;

ANIMALS; BUTTERFLIES; CELL DIVISION; CELL LINE; CELL SURVIVAL; GLYCOSYLATION; INSECTS; ISOENZYMES; MOTHS; RECOMBINANT PROTEINS; SPECIES SPECIFICITY;

ANIMALIA; DANAUS PLEXIPPUS; INSECTA; MAMMALIA; MYTHIMNA UNIPUNCTA; PLEXIPPUS; UNIDENTIFIED BACULOVIRUS; VECTORS;

EID: 0347297507     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp025598o     Document Type: Conference Paper

References (39)

1. Altmann, F.; Staudacher, E.; Wilson, I. B. H.; März, L. Insect cells as hosts for the expression of recombinant glycoproteins. Glycoconjugate J. 1999, 16, 109-123.
2. Palomares, L. A.; Estrada-Mondaca, S.; Ramírez, O. T. Principles and applications of the insect cell-baculovirus expression vector system. In Cell Culture Technology for Pharmaceutical and Cellular Therapies; Ozturk, S., Hu, W. S., Eds.; Marcel Dekker: New York, in press.
3. Breitbach, K.; Jarvis, D. L. Improved glycosylation of a foreign protein by Tn5B1-4 cells engineered to express mammalian glycosyltransferases. Biotechnol. Bioeng. 2001, 74, 230-239.
4. Ailor, E.; Takahashi, N.; Tsukamoto, Y.; Masuda, K.; Rahman, B. A.; Jarvis, D. L.; Lee, Y. C.; Betenbaugh, M. J. N-Glycan patterns of human transferrin produced in Trichoplusia ni insect cells: Effects of mammalian galactosyl-tranferase. Glycobiology 2000, 10, 837-847.
5. Altmann, F.; Schwihla, H.; Staudacher, E.; Glössl, J.; März, L. Insect cells contain an unusual, membrane bound β-N-acetylglucosaminidase probably involved in the processing of protein N-glycans. J. Biol. Chem. 1995, 270, 17344-17349.
6. Tomiya, N.; Ailor, E.; Lawrence, S. M.; Betenbaugh, M. J.; Lee, Y. C. Determination of nucleotides and sugar nucleotides involved in protein glycosylation by high-performance ion exchange chromatography: Sugar nucleotide contents in cultured insect cells and mammalian cells. Anal. Biochem. 2001, 293, 129-137.
7. Jenkins, N.; Parekh, R. B.; James, D. C. Getting the glycosylation right: Implications for the biotechnology industry. Nature Biotechnol. 1996, 14, 975-981.
8. Palomares, L. A.; Ramírez, O. T. Insect cell culture: Recent advances, bioengineering challenges and implications in protein production. In Advances in Bioprocess Engineering; Ramírez, O. T., Galindo, E., Eds.; Kluwer Academic Publishers: Dordrecht, The Netherlands, 1998; Vol. II, pp 25-52.
9. Granados, R. R.; Guoxun, L.; Derksen, A. C. G.; Mackenna, K. A. A new cell line from Trichoplusia ni (BTI-Tn-5B1-4) susceptible to Trichoplusia ni single enveloped nuclear polyhedrosis virus. J. Invert. Pathol. 1994, 64, 642-648.
10. Wang, P.; Toung, R.; Granados, R. R. The establishment of new cell lines from Pseudaletia unipuncta with differential responses to baculovirus infection. In Vitro Cell. Dev. Biol.: Anim. 1999, 35, 333-338.
11. Davis, T. R.; Trotter, K. M.; Granados, R. R.; Wood, H. A. Baculovirus expression of alkaline phosphatase as a reporter gene for evaluation of production, glycosylation and secretion. Bio/Technology 1992, 10, 1148-1150.
12. Hughes, P. R.; Radke, C. D.; Renwick, A. A. A simple, low-input method for continuous laboratory rearing of the monarch butterfly (Lepidoptera, Danaidae) for research. Am. Entomol. 1993, 39, 109-111.
13. Miltenburger, H. G.; Naser, W. L.; Harvey, J. P.; Huber, J.; Huger, A. M. The cellular substrate: A very important requirement for baculovirus in vitro replication. Z. Naturforsch. 1984, 39C, 993-1002.
14. Dee, K. U.; Shuler, M. L. Optimization of an assay for baculovirus titer and design of regimens for synchronous infection of insect cells. Biotechnol. Prog. 1997, 13, 14-24.
15. Kulakosky, P. C.; Shuler, M. L.; Wood, H. A. N-Glycosylation of a baculovirus-expressed recombinant glycoprotein in three insect cell lines. In Vitro Cell. Dev. Biol.: Anim. 1998, 34, 101-108.
16. Joshi, L.; Davis, T. R.; Mattu, T. S.; Rudd, P. M.; Dwek, R. A.; Shuler, M. L.; Wood, H. A. Influence of baculovirus-host cell interactions on complex N-linked glycosylation of a recombinant human protein. Biotechnol. Prog. 2000, 12, 650-656.
17. Guile, G. R.; Rudd, P.; Wing, D. R.; Dwek, R. A. HPLC strategies for profiling and sequencing oligosaccharides. In A Laboratory Guide to Glycoconjugate Analysis; Jackson, P., Clark, D. S., Eds.; Birkhäuser Verlag: Basel, Switzerland, 1997; pp 199-234.
18. Joshi, L.; Shuler, M. L.; Wood H. A. Production of a sialylated N-linked glycoprotein in insect cells. Biotechnol. Prog. 2001, 5, 822-827.
19. Gramer, M. J.; Gooche, C. F. Glycosidase activities in chinese hamster ovary cell lysate and cell culture supernatant. Biotechnol. Prog. 1993, 9, 366-373.
20. Taticek, R. A.; Shuler, M. L. Effect of elevated oxygen and glutamine levels on foreign protein production at high cell densities using the insect cell-baculovirus expression system. Biotechnol. Bioeng. 1997, 54, 142-152.
21. Palomares, L. A.; Ramírez, O. T. The effect of dissolved oxygen tension and the utility of oxygen uptake rate in insect cell culture. Cytotechnology 1996, 22, 225-237.
22. Taticek, R. A.; Choi, C.; Phan, S.-E.; Palomares, L. A.; Shuler, M. L. Comparison of growth and recombinant protein expression in two different insect cell lines in attached and suspension culture. Biotechnol. Prog. 2001, 17, 676-684.
23. Chung, I. S.; Taticek, R. A.; M. L. Shuler. Production of human alkaline phosphatase, a secreted, glycosylated protein, from a baculovirus expression system and the attachment-dependent cell line Trichoplusia ni BTI-Tn5B1-4 using a split-flow, air-lift bioreactor. Biotechnol. Prog. 1993, 9, 675-678.
24. Davis, T. R.; Wickham, T. J.; Mckenna, K. A.; Granados, R. R.; Shuler, M. L.; Wood, H. A. Comparative recombinant protein production of eight insect cell lines. In Vitro Cell. Dev. Biol.: Anim. 1993, 29A, 388-390.
25. Kulakosky, P. C.; Hughes, P. R.; Wood, H. A. N-linked glycosylation of a baculovirus expressed recombinant glycoprotein in insect larvae and tissue culture cells. Glycobiology 1998, 8, 741-745.
26. Varki, A. Factors controlling the glycosylation potential of the Golgi apparatus. Trends Cell Biol. 1998, 8, 34-40.
27. Çayli, A.; Hirschmann, F.; Wurth, M.; Hauser, H.; Wagner, R. Cell lines with reduced UDP-N-acetylhexosamine pool in the presence of ammonium. Biotechnol. Bioeng. 1999, 65, 192-200.
28. Watanabe, S.; Kokuho, T.; Tajahashi, H.; Takahashi, M.; Kubota, T.; Inumaru, S. Sialylation of N-glycans on the recombinant proteins expressed by a baculovirus-insect cell system under β-N-acetylglucosaminidase inhibition. J. Biol. Chem. 2002, 277, 5090-5093.
29. Hsu, T. A.; Takahashi, N.; Tsukamoto, Y.; Kato, K.; Shimada, I.; Masuda, K.; Whiteley, E. M.; Fan, J. Q.; Lee, Y. C.; Betenbaugh, M. J. Differential N-glycan patterns of secreted and intracellular IgG produced in Trichoplusia ni cells. J. Biol. Chem. 1997, 272, 9062-9070.
30. Ogonah, O. W.; Freedman, R. B.; Jenkins, N.; Patel, K.; Rooney, B. C. Isolation and characterization of an insect cell line able to perform complex N-linked glycosylation on recombinant proteins. Bio/technology 1996, 14, 197-202.
31. Davidson, D. J.; Castellino, F. Structures of the asparagines-289-linked oligosaccharides assembled on recombinant human plasminogen expressed in a Mammestra brassicae cell line (IZD-MBO503). Biochemistry 1991, 30, 6689-6696.
32. Davidson, D. J.; Castellino, F. J. Asparagine-linked oligosaccharide processing in lepidopteran insect cells. Temporal dependence of the nature of oligosaccharides assembled on Asparagine-289 of recombinant human plasminogen produced in baculovirus vector infected Spodoptera frugiperda (IPLB-SF-21AE) cells. Biochemistry 1991, 30, 6167-6174.
33. Pajot-Augy, E.; Bozon, V.; Remy, J. J.; Couture, I.; Salesse, R. Critical relationship between glycosylation of recombinant lutropin receptor ectodomain and its secretion from baculovirus infected insect cells. Eur. J. Biochem. 1999, 260, 635-648.
34. Malykh, Y.; Krisch, B.; Gerardy-Schahn, R.; Lapina, E. B.; Shaw, L.; Scauer, R. The presence of N-acetylneuraminic acid in Malpighian tubules of larvae of the cicada Philaenus spumarius. Glycoconj. J. 1999, 16, 731-739.
35. Kim, K.; Lawrence, S. M.; Park, J.; Pitts, L.; Vann, W. F.; Betenbaugh, M. J.; Palter, K. B. Expression of a functional Drosophila melanogaster N-acetylneuraminic acid (Neu5Ac) phosphate synthase gene: Evidence for endogenous sialic acid biosynthetic ability in insects. Glycobiology 2002, 12, 73-83.
36. Donaldson, M.; Wood, H. A.; Kulakosky, P.; Shuler, M. L. Use of mannosamine for inducing the addition of outer arm N-acetylglucosamine onto N-linked oligosaccharides of recombinant proteins in insect cells. Biotechnol. Prog. 1999, 15, 168-173.
37. Lopez, M.; Tetaert, D.; Julaint, S.; Gazon, M.; Cerutti, M.; Verbert, A.; Delannoy, P. O-Glycosylation potential of lepidopteran insect cell lines. Biochim. Biophys. Acta 1999, 1427, 49-61.
38. Donaldson, M, Wood, H. A.; Kulakosky, P. C.; Shuler, M. L. Glycosylation of a recombinant protein in the Tn5B1-4 insect cell line: Influence of ammonia, time of harvest, temperature, and dissolved oxygen. Biotechnol. Bioeng. 1999, 63, 255-262.
39. van Die, A.; van Tetering, H.; van den Eijden, D. H.; Joziasse, D. H. Glycosylation in lepidopteran insect cells: identification of a β-1-4-N-acetylgalactosaminyltransferase involved in the synthesis of complex type oligosaccharide chains. Glycobiology 1996, 6, 157-164.