Cloning and characterization of a thermostable detergent-compatible recombinant keratinase from Bacillus pumilus KS12

Biotechnology and Applied Biochemistry

Volumn 58, Issue 2, 2011, Pages 109-118

  Rajput, Rinky ^a   Sharma, Richa ^a   Gupta, Rani ^a  

^a UNIVERSITY OF DELHI   (India)

Author keywords

Bacillus pumilus; Escherichia coli; keratinase; serine; thiol activated

Indexed keywords

ACTIVATED ENZYMES; AMIDOLYTIC ACTIVITIES; BACILLUS PUMILUS; CHELATING AGENT; DEGRADING BACTERIA; DIETHYLAMINOETHYL; DITHIOTHREITOL; ELECTROSPRAY IONIZATION MASS SPECTROMETRY; ETHYLENE DIAMINE TETRA-ACETIC ACID; FUNCTIONAL EXPRESSION; KERATINASE; LARGE ARRAYS; MERCAPTOETHANOL; O-PHENANTHROLINE; OXIDATION STABILITY; PH RANGE; PHENYLMETHYLSULFONYL FLUORIDE; PROTEIN SUBSTRATE; RELATIVE ACTIVITIES; SERINE; THIOL ACTIVATED; TRITON X-100;

BACILLI; BACTERIOLOGY; CHELATION; CLONING; DETERGENTS; ELECTROSPRAY IONIZATION; ENZYMES; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; HYDROLYSIS; MASS SPECTROMETRY;

AMINO ACIDS;

1,10 PHENANTHROLINE; ALANINE; BENZYLSULFONYL FLUORIDE; BETA CRYSTALLIN; CASEIN; CYSTEINE; DETERGENT; DIETHYLAMINOETHYL CELLULOSE; DITHIOTHREITOL; EDETIC ACID; GLYCINE; INSULIN; KERATINASE; LEUCINE; MERCAPTOETHANOL; PHENYLALANINE; RECOMBINANT ENZYME; RECOMBINANT KERATINASE; SAPONIN; SEPHAROSE; SERINE DEHYDRATASE; THIOL; TRITON X 100; TYROSINE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS PUMILUS; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME DEGRADATION; ENZYME INHIBITION; ENZYME METABOLISM; ENZYME PURIFICATION; ENZYME RELEASE; ENZYME STABILITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; INCUBATION TEMPERATURE; MOLECULAR CLONING; NONHUMAN; PH; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN HYDROLYSIS; THERMOSTABILITY;

BACILLUS PUMILUS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 79955072136     PISSN: 08854513     EISSN: 14708744     Source Type: Journal    
DOI: 10.1002/bab.16     Document Type: Article

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