Comparison of standard culture method and real-time PCR assay for detection of staphylococcus aureus in processed and unprocessed foods

Korean Journal for Food Science of Animal Resources

Volumn 30, Issue 3, 2010, Pages 410-418

  Lee, Jae Hoon ^a   Song, Kwang Young ^a   Hyeon, Ji Yeon ^a   Hwang, In Gyun ^b   Kwak, Hyo Sun ^b   Han, Jeong A ^b   Chung, Yun Hee ^c   Seo, Kun Ho ^a  

^a Konkuk University   (South Korea)

^b Korea Food and Drug Administration   (South Korea)

^c Korea Consumer Agency   (South Korea)

Author keywords

Foods; Real time PCR; Standard culture methods

Indexed keywords

STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

EID: 79954617839     PISSN: 12258563     EISSN: None     Source Type: Journal    
DOI: 10.5851/kosfa.2010.30.3.410     Document Type: Article

References (15)

1. Alarcon, B., Vicedo, B., and Aznar, R. (2006) PCR-based procedure for detection and quantification of Staphylococcus aureus and their application in foods. J. Appl Microbiol. 100, 352-364.
2. Ellender, R. D., Huang, L., Sharp, S. L., and Telleton, R. P. (1995) Isolation, enumeration, and identification of Grampositive cocci from frozen crabmeat. J. Food Prot. 58, 853-857.
3. Goto, M., Takahashi, H., Segawa, Y., Hayashidani, H., Takatori, K., and Hara-Kudo, Y. (2007) Real-time PCR method for quantification of Staphylococcus aureus in milk. J. Food Prot. 70, 90-96. (Pubitemid 46191869)
4. Han, S. R., Hyeon, J. Y., Kim, H. Y., Park, J. S., Heo, S., Shin, H. S., and Seo, K. H. (2008) Evaluation of conventional culture methods and validation of immunoassays for rapid detection of Listeria monocytogenes in dairy and processed foods. Korean J. Food Sci. Ani. Resour. 286, 16-622.
5. Hyeon, J. Y., Hwang, I. G., Kwak, H. S., Park, J. S., Heo, S., Choi, I. S., Park, C. K., and Seo, K. H. (2009) Evaluation of an automated ELISA(VIDAS®) and real-time PCR by comparing with a conventional culture methods for the detection of Salmonella spp. in steamed pork and raw broccoli sprouts. Korean J. Food Sci. Ani. Resour. 29, 506-512
6. Ingham, S. C., Becker, K. L., and Fanslau, M. A. (2003) Comparison of the Baird-Parker agar and 3M Petrifilm Staph Express Count plate methods for enumeration of Staphylo-coccus aureus in naturally and artificially contaminated foods. J. Food Prot. 66, 2151-2155. (Pubitemid 37399789)
7. Kim, M. H., Kim, W. J., Shin, W. S., Shon, D. H., and Cha, S. K. (2003) Feasibility study on the use of liposomes for detecting food-borne pathogenic bacteria. Korean J. Food Sci. Ani. Resour. 23, 278-283.
8. Marin, M. E., de la Rosa, M. C., and Cornejo, I. (1992) Enterotoxigenicity of Staphylococcus strains isolated from Spanish dry-cured hams. Appl. Environ. Microbiol. 58, 1067-1069.
9. Moon, J. Y., Lee, E. J., and Kim, Y. B. (2004) Rapid detection of methicilin resistant Staphylococcus aureus by multiplex PCR. J. Bacteriol. Virol. 34, 91-100. (Pubitemid 41224637)
10. Much, P., Pichler, J., Kasper, S. S., and Allerberger, F. (2009) Foodborne outbreaks, Austria 2007. Wien Klin Wochenschr. 121, 77-85
11. Palomares, C., Torres, M. J., Torres, A., Aznar, J., and Palomares, J. C. (2003) Rapid detection and identification of Staphylococcus aureus from blood culture specimens using real-time fluorescence PCR. Diagn. Microbiol. Infect. Dis. 45, 183-189. (Pubitemid 36349658)
12. Rall, V. L. M., Vieira, F. P., Rall, R., Vieitis, R. L., Fernandes, Jr., Candeias, J. M. G., Cardoso, K. F. G., and Araujo, Jr. J. P. (2008) PCR detection of Staphylococcal enterotoxin genes in Staphylococcus aureus strains isolated from raw and pasteurized milk. Vet. Microbiol. 132, 408-413.
13. Riyaz-Ul-Hassan, S., Verma, V., and Qazi, G. N. (2008) Evaluation of three different molecular markers for the detection of Staphylococcus aureus by polymerase chain reaction. Food Microbiol. 25, 452-459.
14. Suarez, M. J., Arias, M. L., and del Mar Gamboa, M. (2008) Staphylococcus aureus enterotoxin A detection using the polymerase chain reaction (PCR) and its correlation with coagulase and thermonuclease tests. Arch. Latinoam. Nutr. 58, 59-63.
15. Yoon, J. H., Lee, S. J., and Hong, K. W. (2008) A multiplex PCR assay for the direct detection of Salmonella spp., Yersinia enterocolitica, and Staphylococcus aureus in food. Food Eng. Prog. 12, 24-31.