Simultaneous assessment of cytochrome P450 activity in cultured human hepatocytes for compound-mediated induction of CYP3A4, CYP2B6, and CYP1A2

Journal of Pharmacological and Toxicological Methods

Volumn 63, Issue 3, 2011, Pages 223-226

  Rhodes, Susan P ^a   Otten, Jennifer N ^a   Hingorani, Gary P ^a   Hartley, Dylan P ^a,b   Franklin, Ronald B ^a  

^a ARRAY BIOPHARMA INC   (United States)

^b GENENTECH INC   (United States)

Author keywords

Cytochrome P450 induction; Liquid chromatography tandem mass spectrometry; Primary human hepatocytes; Substrate cocktail

Indexed keywords

3 METHYLCHOLANTHRENE; ALPHA HYDROXYMIDAZOLAM; AMFEBUTAMONE; AROMATIC HYDROCARBON RECEPTOR; ARTEMISIN; CONSTITUTIVE ANDROSTANE RECEPTOR; CYTOCHROME P450; CYTOCHROME P450 1A2; CYTOCHROME P450 2B6; CYTOCHROME P450 3A4; MIDAZOLAM; OMEPRAZOLE; PARACETAMOL; PHENACETIN; PHENOBARBITAL; PREGNANE X RECEPTOR; RADAFAXINE; RIFAMPICIN;

ARTICLE; CELL VIABILITY; CONTROLLED STUDY; DRUG SELECTIVITY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INDUCTION; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; LIQUID CHROMATOGRAPHY; LIVER CELL CULTURE; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; SENSITIVITY ANALYSIS; TANDEM MASS SPECTROMETRY; XENOBIOTIC STRESS;

ARYL HYDROCARBON HYDROXYLASES; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CELLS, CULTURED; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYTOCHROME P-450 CYP1A2; CYTOCHROME P-450 CYP3A; DRUG DISCOVERY; ENZYME INDUCTION; HEPATOCYTES; HUMANS; LIGANDS; OXIDOREDUCTASES, N-DEMETHYLATING; RECEPTORS, ARYL HYDROCARBON; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECEPTORS, STEROID; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SUBSTRATE SPECIFICITY; TANDEM MASS SPECTROMETRY; XENOBIOTICS;

EID: 79954612110     PISSN: 10568719     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vascn.2010.11.002     Document Type: Article

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