Comparison of the standard culture method and real-time PCR for the detection of vibrio parahaemolyticus in seafoods and vegetables

Korean Journal of Food Science and Technology

Volumn 42, Issue 3, 2010, Pages 355-360

  Chon, Jung Whan ^a   Hyeon, Ji Yeon ^a   Hwang, In Gyun ^b   Kwak, Hyo Sun ^b   Han, Jeong A ^b   Chung, Yun Hee ^c   Song, Kwang Young ^a   Seo, Kun Ho ^a  

^a Konkuk University   (South Korea)

^b Korea Food and Drug Administration   (South Korea)

^c Test and Research Center   (South Korea)

Author keywords

Comparison; Culture method; Real time PCR; Vibrio parahaemolyticus

Indexed keywords

RAPHANUS SATIVUS; VIBRIO PARAHAEMOLYTICUS;

EID: 79954572962     PISSN: 03676293     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Joseph SW, Cowell RR, Kaper JB. Vibrio parahaemolyticus and related halophilic vibrios. Crit. Rev. Microbiol. 10: 77-123 (1983)
2. Su YC, Liu C. Vibrio parahaemolyticus: A concern of seafood safety. Food Microbiol. 24: 549-558 (2007) (Pubitemid 46517975)
3. Morris JG. Cholera and other types of vibriosis: A story of human pandemics and oysters on the half shell. Clin. Infect. Dis. 37: 272-280 (2003)
4. DePaola A, Hopkin LH, Peeler JT, Wentz B, McPhearson RM. Incidence of Vibrio parahaemolyticus in US coastal waters and oysters. Appl. Environ. Microb. 56: 2299-2302(1990) (Pubitemid 20235666)
5. Jung SH, Hur MJ, Ju JH, Kim KA, Oh SS, Go JM, Kim YH, Im JS. Microbiological evaluation of raw vegetables. J. Food Hyg. Safety, 21: 250-257 (2006)
6. Kim MH, Shin WS. Microbiological quality of raw and cooked foods in middle and high school food service establishments. J. Korean Soc. Food Sci. Nutr. 37: 1343-1356 (2008)
7. Korea Food and Drug Administration. Food & Drug Statistical Yearbook for 2009. Available from http://www.kfda.go.kr/index.kfda?mid=96&pageNo= 1&seq=7181&cmd=v, Accessed Nov. 10, 2009.
8. Korea Food and Drug Administration. Food code. Available from http://safefood.kfda.go.kr/RS/food-menu.jsp, Accessed Nov. 21,2009.
9. Ward LN, Bej AK. Detection of Vibrio parahaemolyticus in shellfish by use of multiples real-time PCR with TaqMan® fluorescent probes. Appl. Environ Microb. 72: 2031-2042 (2006)
10. Yang C, Jiang Y, Huang K, Zhu C, Yin Y. Application of realtime PCR for quantitative detection of Campylobacter jejuni in poultry, milk, and environmental water. FEMS Immunol Med Mic. 38: 265-271 (2003) (Pubitemid 37289751)
11. Nordstrom JL, Vickery MC, Blackstone GM, Murray SL, DePaola A. Development of a multiplex real-time PCR assay with an internal amplification control for the detection of total and pathogenic Vibrio parahaemolyticus bacteria in oysters. Appl. Environ Microb. 73: 5840-5847 (2007) (Pubitemid 47462996)
12. Honda T, Iida T. The pathogenicity of Vibrio parahaemolyticus and the role of the thermostable direct hemolysin and related hemolysins. Rev. Med. Microbiol. 4: 106-113 (1993) (Pubitemid 23131684)
13. Hondo S, Goto I, Minematsu I, Ikeda N, Asano N, Ishibashi M, Kinoshita Y, Nishibuchi N, Honda T, Miwatani T. Gastroenteritis due to Kanagawa negative Vibrio parahaemolyticus. Lancet 1: 331-332 (1987) (Pubitemid 17009821)
14. Dileep V, Kumar HS, Kumar Y, Nishibuchi M, Karunasagar I, Karunasagar I. Application of polymerase chain reaction for the detection of Vibrio parahaemolyticus associated with tropical seafoods and coastal environment. Lett. Appl. Microbiol. 36: 423-427 (2003) (Pubitemid 36649088)
15. Kim YB, Okuda J, Matsumoto C, Takahashi N, Hashimoto S, Nishibuchi M. Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the tox R gene. J. Clin. Microbiol. 37: 1173-1177(1999) (Pubitemid 29140634)
16. Thompson CA, Vanderzant C. Serological and hemolytic characteristics of Vibrio parahaemolyticus from marine sources. J. Food Sci. 41: 150-151(1976)
17. Kawatsu K, Ishibashi M, Tsukamoto T. Development and evaluation of a rapid, simple, and sensitive immunochromatographic assay to detect thermostable direct hemolysin produced by Vibrio parahaemolyticus in enrichment cultures of stool specimens. J. Clin. Microbiol. 44: 1821-1827 (2006) (Pubitemid 43726274)
18. Blackstone GM, Nordstrom JL, Vickery MC, Bowen MD, Meyer RF, DePaola A. Detection of pathogenic Vibrio parahaemolyticus in oyster enrichment by real-time PCR. J. Microbiol. Methods 53: 149-155 (2003) (Pubitemid 36332349)
19. Hyeon JY, Hwang IG, Kawk HS, Park JS, Heo S, Choi IS, Park CK, Seo KH. Evaluation of an automated ELISA and real-time PCR by comparing with a conventional culture method for the detection of Salmonella spp. in steamed pork and raw broccoli sprouts. Korean J. Food Sci. Anim. Resour. 29: 506-512 (2009)
20. Seo KH, Brackett RE. Rapid, specific detection of Enterobacter sakazakii in infant formula using a real-time PCR assay. J. Food Protect. 68: 59-63 (2005) (Pubitemid 40113154)
21. Vold L, Holck A, Wasteson Y, Nissen H. High levels of background flora inhibits growth of Escherichia coli O157:H7 in ground beef. Int. J. Food Microbiol, 56: 219-225 (2000) (Pubitemid 30251855)
22. Malorny B, Paccassoni E, Fach P, Bunge C, Martin A, Helmuth R. Diagnostic real-time PCR for detection of Salmonella in food. Appl. Environ Microb. 70: 7064-7052 (2004)
23. Bolinches J, Romalde JL, Toranzo AE. Evaluation of selective media for isolation and enumeration of vibrios from estrarine waters. J. Microbiol. Meth. 8: 151-160 (1988)
24. Alam MJ, Tomochika K, Miyoshi S, Shinoda S. Analysis of seawaters for the recovery of culturable Vibrio parahaemolyticus and some other vibrios. Microbiol. Immunol. 45: 393-397 (2001) (Pubitemid 32496809)
25. Bottone EJ, Robin T. Vibrio parahaemolyticus: Suspicion of presence based on aberrant biochemical and morphologicsl features. J. Clin. Microbiol. 8: 760-763 (1978) (Pubitemid 9073802)