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Crystal Growth and Design
Volumn 11, Issue 4, 2011, Pages 1182-1192
Membrane protein crystallization in lipidic mesophases. Hosting lipid effects on the crystallization and structure of a transmembrane peptide
Author keywords

[No Author keywords available]
Indexed keywords

BI-LAYER; CURRENT STRUCTURE; DOUBLE HELIX; HIGH QUALITY; L-AMINO ACIDS; LIPID ACYL-CHAINS; MEMBRANE PROTEINS; MESOPHASES; MONOVALENT CATIONS; ONE-FORM; SHAPE AND SIZE; STRUCTURE DETERMINATION; TRANSMEMBRANES;
EID: 79953842881     PISSN: 15287483     EISSN: 15287505     Source Type: Journal    
DOI: 10.1021/cg101384p     Document Type: Article
Times cited : (19)
References (54)
  • 53
    • 79953898443 scopus 로고    scopus 로고
    • note
    • The in meso crystallogenesis reported on herein makes use of MAGs containing cis monounsaturated fatty acids. A shorthand system for describing the chemical constitution of these lipids is referred to as the N.T MAG notation. (52) This is based on a simplistic view of the MAG molecule as an object consisting of a head, a neck, and a tail with the latter two joined by a trunk. Here the head of the MAG is the glycerol headgroup. It is in an ester linkage to the neck corresponding to that part of the acyl chain extending from its carboxyl carbon to the first carbon of the olefin. The trunk is the cis double bond. The tail extends from the second carbon of the olefin to the chains methyl terminus. In the N.T MAG notation, N and T correspond to the number of carbon atoms in the neck and tail, respectively. The total number of carbon atoms in the chain is the sum of N and T. Thus, 11.7 MAG represents monovaccenin, a MAG with a fatty acyl chain 18 carbon atoms long where the cis double bond resides between carbon atoms 11 and 12. It is an olefinic isomer of 9.9 MAG commonly known as monoolein.
  • 54
    • 79953858817 scopus 로고    scopus 로고
    • In a separate study using circular dichroism, we obtained evidence that gramicidin exists in the HHSH form when reconstituted into the bilayer of the cubic phase. (23) However, in that study a different reconstitution protocol was used which involved dissolving the peptide directly in the molten lipid. Thus, the peptide was not exposed to solvent as was the case in the current study. As noted, exposure to solvent may have an effect on the conformation adopted by gramicidin, and so a direct comparison between the two studies is not appropriate.
    • In a separate study using circular dichroism, we obtained evidence that gramicidin exists in the HHSH form when reconstituted into the bilayer of the cubic phase. (23) However, in that study a different reconstitution protocol was used which involved dissolving the peptide directly in the molten lipid. Thus, the peptide was not exposed to solvent as was the case in the current study. As noted, exposure to solvent may have an effect on the conformation adopted by gramicidin, and so a direct comparison between the two studies is not appropriate.

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.