Refractive index sensing of green fluorescent proteins in living cells using fluorescence lifetime imaging microscopy

Biophysical Journal

Volumn 94, Issue 8, 2008, Pages

  Van Manen, Henk Jan ^a   Verkuijlen, Paul ^b   Wittendorp, Paul ^a   Subramaniam, Vinod ^a   Van Den Berg, Timo K ^b   Roos, Dirk ^b   Otto, Cees ^a  

^a UNIVERSITY OF TWENTE   (Netherlands)

^b UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN;

CELL FUNCTION; COMPUTER ASSISTED DIAGNOSIS; FLUORESCENCE MICROSCOPY; LETTER; METHODOLOGY; PHYSIOLOGY; REFRACTOMETRY;

CELL PHYSIOLOGY; GREEN FLUORESCENT PROTEINS; IMAGE INTERPRETATION, COMPUTER-ASSISTED; MICROSCOPY, FLUORESCENCE; REFRACTOMETRY;

EID: 43149091227     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1529/biophysj.107.127837     Document Type: Article

References (19)

1. Festy, F., S. M. Ameer-Beg, T.Ng, and K. Suhling. 2007. Imaging proteins in vivo using fluorescence lifetime microscopy. Mol. Biosyst. 3:381-391.
2. Van Munster, E. B., and T. W. J. Gadella. 2005. Fluorescence lifetime imaging microscopy. Adv. Biochem. Eng. Biotechnol. 95:143-175.
3. Suhling, K., D. M. Davis, and D. Phillips. 2002. The influence of solvent viscosity on the fluorescence decay and time-resolved anisotropy of green fluorescent protein. J. Fluoresc. 12:91-95.
4. Suhling, K., J. Siegel, D. Phillips, P. M. W. French, S. Lévêque-Fort, S. E. D. Webb, and D. M. Davis. 2002. Imaging the environment of green fluorescent protein. Biophys. J. 83:3589-3595.
5. Borst, J. W., M.A. Hink, A. van Hoek, and A. J. W. G.Visser. 2005. Effects of refractive index and viscosity on fluorescence and anisotropy decays of enhanced cyan and yellow fluorescent proteins. J. Fluoresc. 15:153-160.
6. Strickler, S. J., and R. A. Berg. 1962. Relationship between absorption intensity and fluorescence lifetime of molecules. J. Chem. Phys. 37:814-882.
7. Campbell, R. E., O. Tour, A. E. Palmer, P. A. Steinbach, G. S. Baird, D. A. Zacharias, and R. Y. Tsien. 2002. A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. USA. 99:7877-7882.
8. Cross, A. R., and A. W. Segal. 2004. The NADPH oxidase of professional phagocytes - prototype of the NOX electron transport chain systems. Biochim. Biophys. Acta. 1657:1-22.
9. phox during phagocytosis. J. Biol. Chem. 279:9097-9102.
10. Van Manen, H.-J., R. Van Bruggen, D. Roos, and C. Otto. 2006. Single-cell optical imaging of the phagocyte NADPH oxidase. Antioxid. Redox Signal. 8:1509-1522.
11. Bokoch, G. M., and T. Zhao. 2006. Regulation of the phagocyte NADPH oxidase by Rac GTPase. Antioxid. Redox Signal. 8:1533-1548.
12. phox is synthesized as a 65-kDa precursor (p65) in the endoplasmic reticulum. J. Biol. Chem. 274:4364-4369.
13. 558 using site-directed mutagenesis. J. Biol. Chem. 273:6575-6581.
14. phox/Nox2 in COS-7 cells: cellular localization of the protein and the detection of outward proton currents. Biochem. J. 385:649-657.
15. Hanley, Q. S., V. Subramaniam, D. J. Arndt-Jovin, and T. M. Jovin. 2001. Fluorescence lifetime imaging: multi-point calibration, minimum resolvable differences, and artifact suppression. Cytometry. 43:248-260.
16. Curl, C. L., C. J. Bellair, T. Harris, B. E. Allman, P. J. Harris, A. G. Stewart, A. Roberts, K. A. Nugent, and L. M. D. Delbridge. 2005. Refractive index measurements in viable cells using quantitative phaseamplitude microscopy and confocal microscopy. Cytometry A. 65:88-92.
17. Choi, W., C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld. 2007. Tomographic phase microscopy. Nat. Methods. 4:717-719.
18. Beuthan, J., O. Minet, J. Helfmann, M. Herrig, and G. Müller. 1996. The spatial variation of the refractive index in biological cells. Phys. Med. Biol. 41:369-382.
19. Treanor, B., P. M. P. Lanigan, S. Kumar, C. Dunsby, I. Munro, E. Auksorius, F. J. Culley, M. A. Purbhoo, D. Phillips, M. A. A. Neil, D. N. Burshtyn, P. M. W. French, and D. M. Davis. 2006. Microclusters of inhibitory killer immunoglobulin-like receptor signaling at natural killer cell immunological synapses. J. Cell Biol. 174:153-161.