Nondividing cells: A safer bet for integrating vectors?

Molecular Therapy

Volumn 19, Issue 4, 2011, Pages 640-641

  Brady, Troy ^a   Bushman, Frederic D ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HIGH MOBILITY GROUP A2 PROTEIN; LENS EPITHELIUM DERIVED GROWTH FACTOR; LENTIVIRUS VECTOR; PARVOVIRUS VECTOR; RETROVIRUS VECTOR;

BETA THALASSEMIA; CELL CYCLE ARREST; CELL DIVISION; CELL TRANSFORMATION; GENE INSERTION; GENE THERAPY; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; NERVE CELL; NONHUMAN; NOTE; PROTEIN BINDING; RETINA CELL; VIRAL GENE DELIVERY SYSTEM; VIRUS DNA CELL DNA INTERACTION;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; ANIMALS; CELL LINE; DNA, SATELLITE; FEMALE; GENETIC VECTORS; LENTIVIRUS; MICE; MICE, INBRED BALB C; MITOSIS; POLYMERASE CHAIN REACTION; RATS; TERMINAL REPEAT SEQUENCES; TRANSCRIPTION FACTORS; VIRUS INTEGRATION;

EID: 79953293958     PISSN: 15250016     EISSN: 15250024     Source Type: Journal    
DOI: 10.1038/mt.2011.40     Document Type: Note

References (16)

1. Bartholomae, CC, Arens, A, Balaggan, KS, Yáñez-Muñoz, RJ, Montini E, Howe, SJ et al. (2011). Lentiviral vector integration profiles differ in rodent postmitotic tissues. Mol Ther 19: 703-10.
2. Ciuffi, A, Llano, M, Poeschla, E, Hoffmann, C, Leipzig, J, Shinn, P et al. (2005). A role for LEDGF/ p75 in targeting HIV DNA integration. Nat Med 11: 1287-1289. (Pubitemid 41752906)
3. Miller, DG, Trobridge, GD, Petek, LM, Jacobs, MA, Kaul, R and Russell, DW (2005). Large-scale analysis of adeno-associated virus vector integration sites in normal human cells. J Virol 79: 11434-11442. (Pubitemid 41170684)
4. Cavazzana-Calvo, M, Payen, E, Negre, O, Wang, G, Hehir, K, Fusil, F et al. (2010). Transfusion independence and HMGA2 activation after gene therapy of human beta-thalassaemia. Nature 467: 318-322.
5. Cartier, N, Hacein-Bey-Abina, S, Bartholomae, C, Veres, G, Schmidt, M, Kutschera, I et al. (2009). Hematopoietic stem cell gene therapy with a lentiviral vector in X-linked adrenoleukodystrophy. Science 326: 818-823.
6. Shun, MC, Raghavendra, NK, Vandegraaff, N, Daigle, JE, Hughes, S, Kellam, P et al. (2007). LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration. Genes Dev 21: 1767-1778. (Pubitemid 47076476)
7. Marshall, H, Ronen, K, Berry, C, Llano, M, Sutherland, H, Saenz, D et al. (2007). Role of PSIP1/LEDGF/p75 in lentiviral infectivity and integration targeting. PLoS One 2: e1340.
8. Llano, M, Saenz, DT, Meehan, A, Wongthida, P, Peretz, M, Walker, WH et al. (2006). An essential role for LEDGF/p75 in HIV integration. Science 314: 461-464. (Pubitemid 44628966)
9. Cherepanov, P, Devroe, E, Silver, PA and Engelman, A (2004). Identification of an evolutionarily conserved domain of LEDGF/p75 that bind HIV-1 integrase. J Biol Chem 279: 48883-48892.
10. Poeschla, EM (2008). Integrase, LEDGF/p75 and HIV replication. Cell Mol Life Sci 65: 1403-1424.
11. Ciuffi, A, Mitchell, RS, Hoffmann, C, Leipzig, J, Shinn, P, Ecker, JR et al. (2006). Integration site selection by HIV-based vectors in dividing and growth-arrested IMR-90 lung fibroblasts. Mol Ther 13: 366-373. (Pubitemid 43056902)
12. Barr, SD, Ciuffi, A, Leipzig, J, Shinn, P, Ecker, JR and Bushman, FD (2006). HIV integration site selection: targeting in macrophages and the effects of different routes of viral entry. Mol Ther 14: 218-225. (Pubitemid 43942348)
13. Christ, F, Voet, A, Marchand, A, Nicolet, S, Desimmie, BA, Marchand, D et al. (2010). Rational design of small-molecule inhibitors of the LEDGF/p75-integrase interaction and HIV replication. Nat Chem Biol 6: 442-448.
14. Gijsbers, R, Ronen, K, Vets, S, Malani, N, De Rijck, J, McNeely, M et al. (2010). LEDGF hybrids efficiently retarget lentiviral integration into heterochromatin. Mol Ther 18: 552-560.
15. Ferris, AL, Wu, X, Hughes, CM, Stewart, C, Smith, SJ, Milne, TA et al. (2010). Lens epithelium-derived growth factor fusion proteins redirect HIV-1 DNA integration. Proc Natl Acad Sci USA 107: 3135-3140.
16. Silvers, RM, Smith, JA, Schowalter, M, Litwin, S, Liang, Z, Geary, K et al. (2010). Modification of integration site preferences of an HIV-1-based vector by expression of a novel synthetic protein. Hum Gene Ther 21: 337-349.