Transcriptome, proteome, and metabolite analyses of a lactate dehydrogenase-negative mutant of Enterococcus faecalis V583

Applied and Environmental Microbiology

Volumn 77, Issue 7, 2011, Pages 2406-2413

  Mehmeti, Ibrahim ^a   Jönsson, Maria ^a   Fergestad, Ellen M ^b   Mathiesen, Geir ^c   Nes, Ingolf F ^a   Holo, Helge ^a,d  

^a Lab of Microbial Gene Technology   (Norway)

^b NOFIMA   (Norway)

^c NORWEGIAN UNIVERSITY OF LIFE SCIENCES   (Norway)

^d TINE SA   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

ACETOIN; CATABOLIC GENES; DIFFERENTIALLY EXPRESSED GENE; ENERGY METABOLISM; ENTEROCOCCUS FAECALIS; GEL ANALYSIS; GENES ENCODE; LACTATE DEHYDROGENASE; METABOLITE ANALYSIS; MICROARRAY ANALYSIS; NEGATIVE MUTANTS; PROTEOMES; PYRUVATES; REGULATORY NETWORK; STAPHYLOCOCCUS AUREUS; TRANSCRIPTOMES; WILD TYPES;

BINDING SITES; BIOCHEMISTRY; ENZYMES; ETHANOL; GENES; GLUCOSE; METABOLISM; PHYSIOLOGY;

TRANSCRIPTION;

ACETOIN; FORMIC ACID; FORMIC ACID DERIVATIVE; GLUCOSE; LACTATE DEHYDROGENASE; NICOTINAMIDE ADENINE DINUCLEOTIDE; PROTEOME;

BACTERIUM; ENZYME ACTIVITY; ETHANOL; METABOLISM; METABOLITE; PROTEOMICS;

ARTICLE; CHEMISTRY; ENTEROCOCCUS FAECALIS; ENZYMOLOGY; FERMENTATION; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; METABOLOME; MICROARRAY ANALYSIS; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ACETOIN; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ENTEROCOCCUS FAECALIS; FERMENTATION; FORMIC ACIDS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, BACTERIAL; GLUCOSE; L-LACTATE DEHYDROGENASE; METABOLOME; MICROARRAY ANALYSIS; NAD; PROTEOME;

ENTEROCOCCUS FAECALIS; STAPHYLOCOCCUS AUREUS;

EID: 79953273591     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02485-10     Document Type: Article

References (34)

1. Bøhle, L. A., et al. 2010. Identification of proteins related to the stress response in Enterococcus faecalis V583 caused by bovine bile. Proteome Sci. 8:37.
2. Brekasis, D., and M. Paget. 2003. A novel sensor of NADH/NAD+ redox poise in Streptomyces coelicolor A3(2). EMBO J. 22:4856-4865.
3. Dressaire, C., et al. 2009. Transcriptome and proteome exploration to model translation efficiency and protein stability in Lactococcus lactis. PLoS Comput. Biol. 5:e1000606.
4. Even, S., C. Garrigues, P. Loubiere, N. D. Lindley, and M. Cocaign-Bousquet. 1999. Pyruvate metabolism in Lactococcus lactis is dependent upon glyceraldehyde-3-phosphate dehydrogenase activity. Metab. Eng. 1:198-205.
5. Even, S., N. D. Lindley, and M. Cocaign-Bousquet. 2003. Transcriptional, translational and metabolic regulation of glycolysis in Lactococcus lactis subsp. cremoris MG 1363 grown in continuous acidic cultures. Microbiology 149:1935-1944.
6. Færgestad, E. M., et al. 2007. Pixel-based analysis of multiple images for the identification of changes: a novel approach applied to unravel proteome patterns of 2-D electrophoresis gel images. Proteomics 7:3450-3461.
7. Fuchs, S., J. Pané-Farré, C. Kohler, M. Hecker, and S. Engelmann. 2007. Anaerobic gene expression in Staphylococcus aureus. J. Bacteriol. 189:4275-4289.
8. Giard, J. C., et al. 2006. Characterization of Ers, a PrfA-like regulator of Enterococcus faecalis. FEMS Immunol. Med. Microbiol. 46:410-418.
9. Guillot, A., C. Gitton, P. Anglade, and M. Y. Mistou. 2003. Proteomic analysis of Lactococcus lactis, a lactic acid bacterium. Proteomics 3:337-354.
10. Gyan, S., Y. Shiohira, I. Sato, M. Takeuchi, and T. Sato. 2006. Regulatory loop between redox sensing of the NADH/NAD(+) ratio by Rex (YdiH) and oxidation of NADH by NADH dehydrogenase Ndh in Bacillus subtilis. J. Bacteriol. 188:7062-7071.
11. Jensen, N. B. S., C. R. Melchiorsen, K. V. Jokumsen, and J. Villadsen. 2001. Metabolic behavior of Lactococcus lactis MG1363 in microaerobic continuous cultivation at a low dilution rate. Appl. Environ. Microbiol. 67:2677-2682.
12. Jönsson, M., Z. Saleihan, I. F. Nes, and H. Holo. 2009. Construction and characterization of three lactate dehydrogenase-negative Enterococcus faecalis V583 mutants. Appl. Environ. Microbiol. 75:4901-4903.
13. Larsen, R., S. A. van Hijum, J. Martinussen, O. P. Kuipers, and J. Kok. 2008. Transcriptome analysis of the Lactococcus lactis ArgR and AhrC regulons. Appl. Environ. Microbiol. 74:4768-4771.
14. Marsili, R. T. 1981. Monitoring bacterial metabolites in cultured buttermilk by high performance liquid chromatography and headspace gas chromatography. J. Chromatogr. Sci. 19:451.
15. Melchiorsen, C. R., K. V. Jokumsen, J. Villadsen, H. Israelsen, and J. Arnau. 2002. The level of pyruvate-formate lyase controls the shift from homolactic to mixed-acid product formation in Lactococcus lactis. Appl. Microbiol. Biotechnol. 58:338-344.
16. Mickelson, M. N. 1964. Chemically defined medium for growth of Streptococcus pyogenes. J. Bacteriol. 88:158-164.
17. Narvhus, J. A., K. Thorvaldsen, and R. K. Abrahamsen. 1990. Quantitative determination of volatile compounds produced by Lactococcus ssp. using direct automatic headspace gas chromatography, p. 522. In Dairying in a changing world: XXIII International Dairy Congress. IDF, Brussels, Belgium.
18. Opsata, M., I. F. Nes, and H. Holo. 2010. Class IIa bacteriocin resistance in Enterococcus faecalis V583: the mannose PTS operon mediates global transcriptional responses. BMC Microbiol. 10:224.
19. Pagels, M., et al. 2010. Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus. Mol. Microbiol. 76:1142-1161.
20. Palmfeldt, J., M. Paese, B. Hahn-Hägerdal, and E. W. J. van Niel. 2004. The pool of ADP and ATP regulates anaerobic product formation in resting cells of Lactococcus lactis. Appl. Environ. Microbiol. 70:5477-5484.
21. Paulsen, I. T., et al. 2003. Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis. Science 299:2071-2074.
22. Platteeuw, C., J. Hugenholtz, M. Starrenburg, I. van Alen-Boerrigter, and W. M. de Vos. 1995. Metabolic engineering of Lactococcus lactis: influence of the overproduction of alpha-acetolactate synthase in strains deficient in lactate dehydrogenase as a function of culture conditions. Appl. Environ. Microbiol. 61:3967-3971.
23. Ramirez-Arcos, S., M. Liao, S. Marthaler, M. Rigden, and J.-A. R. Dillon. 2005. Enterococcus faecalis divIVA: an essential gene involved in cell division, cell growth and chromosome segregation. Microbiology 151:1381-1393.
24. Rappsilber, J., Y. Ishihama, and M. Mann. 2002. Stop and go extraction tips for matrix-assisted laser desorption/ionization, nanoelectrospray, and LC/MS sample pretreatment in proteomics. Anal. Chem. 75:663-670.
25. Schau, M., Y. Chen, and F. M. Hulett. 2004. Bacillus subtilis YdiH is a direct negative regulator of the cydABCD operon. J. Bacteriol. 186:4585-4595.
26. Shepard, B. D., and M. S. Gilmore. 2002. Differential expression of virulence-related genes in Enterococcus faecalis in response to biological cues in serum and urine. Infect. Immun. 70:4344-4352.
27. Smyth, G. K. 2004. Linear models and empirical Bayes methods for assessing differential expression in microarray experiments. Stat. Appl. Genet. Mol. Biol. 3:Article 3.
28. Smyth, G. K., J. Michaud, and H. S. Scott. 2005. Use of within-array replicate spots for assessing differential expression in microarray experiments. Bioinformatics 21:2067-2075.
29. Smyth, G. K., and T. Speed. 2003. Normalization of cDNA microarray data. Methods 31:265-273.
30. Snoep, J. L., M. R. de Graef, M. J. T. de Mattos, and O. M. Neijssel. 1994. Effect of culture conditions on the NADH/NAD ratio and total amounts of NAD(H) in chemostat cultures of Enterococcus faecalis NCTC 775. FEMS Microbiol. Lett. 116:263-268.
31. Snoep, J. L., M. J. Teixeira de Mattos, P. W. Postma, and O. M. Neijssel. 1990. Involvement of pyruvate dehydrogenase in product formation in pyruvate-limited anaerobic chemostat cultures of Enterococcus faecalis NCTC 775. Arch. Microbiol. 154:50-55.
32. Solheim, M., A. Aakra, L. G. Snipen, D. A. Brede, and I. F. Nes. 2009. Comparative genomics of Enterococcus faecalis from healthy Norwegian infants. BMC Genomics 10:194.
33. Solheim, M., A. Aakra, H. Vebø, L. Snipen, and I. F. Nes. 2007. Transcriptional responses of Enterococcus faecalis V583 to bovine bile and sodium dodecyl sulfate. Appl. Environ. Microbiol. 73:5767-5774.
34. Ward, D. E., et al. 2000. Branched-chain alpha-keto acid catabolism via the gene products of the bkd operon in Enterococcus faecalis: a new, secreted metabolite serving as a temporary redox sink. J. Bacteriol. 182: 3239-3246.