Expression, purification, and improved antigenic specificity of a truncated recombinant bp26 protein of Brucella melitensis M5-90: A potential antigen for differential serodiagnosis of brucellosis in sheep and goats

Biotechnology and Applied Biochemistry

Volumn 58, Issue 1, 2011, Pages 32-38

  Liu, Wen Xing ^a,b   Hu, Sen ^b   Qiao, Zu Jian ^b   Chen, Wei Ye ^b   Liu, Lin Tao ^b   Wang, Fang Kun ^b   Hua, Rong Hong ^b   Bu, ZHi Gao ^b   Li, Xiang Rui ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

^b AGRO ENVIRONMENTAL PROTECTION INSTITUTE   (China)

Author keywords

Antigenic specificity; Brucellosis; Differential serodiagnosis; Purification; Sheep and goats; Truncated recombinant bp26 protein (r bp26)

Indexed keywords

ANTIGENIC SPECIFICITY; BRUCELLA; BRUCELLOSIS; DIFFERENTIAL SERODIAGNOSIS; ENZYME LINKED IMMUNOSORBENT ASSAY; HOST CELLS; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IMMUNOGENICITY; LIVE ATTENUATED VACCINES; RECOMBINANT PLASMID; RECOMBINANT PROTEIN; SHEEP AND GOATS; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; VACCINE STRAINS; WESTERN BLOTS;

AFFINITY CHROMATOGRAPHY; ANIMALS; ANTIBODIES; CHEMICAL DETECTION; ELECTROPHORESIS; ESCHERICHIA COLI; GENES; PURIFICATION; SIZE EXCLUSION CHROMATOGRAPHY; SODIUM; SODIUM SULFATE; VACCINES; WOOL;

ANTIGENS;

ANTIGEN; DODECYL SULFATE SODIUM; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ANIMAL EXPERIMENT; ANTIBODY DETECTION; ANTIGEN SPECIFICITY; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BRUCELLA MELITENSIS; BRUCELLOSIS; CLONING; DRUG EFFICACY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GEL ELECTROPHORESIS; GEL PERMEATION CHROMATOGRAPHY; GENE; GOAT; HOST CELL; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IMMUNOGENICITY; MALE; MOLECULAR WEIGHT; MUTANT; NONHUMAN; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RECOMBINANT PLASMID; SERODIAGNOSIS; SEROLOGY; SHEEP; WESTERN BLOTTING;

ANIMALS; BRUCELLA MELITENSIS; BRUCELLA VACCINE; BRUCELLOSIS; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES; GENE EXPRESSION REGULATION, BACTERIAL; GOAT DISEASES; GOATS; MEMBRANE PROTEINS; RECOMBINANT PROTEINS; SEROLOGIC TESTS; SHEEP; SHEEP DISEASES;

ANIMALIA; BRUCELLA; BRUCELLA MELITENSIS; CAPRA HIRCUS; ESCHERICHIA COLI; OVIS ARIES;

EID: 79953252190     PISSN: 08854513     EISSN: 14708744     Source Type: Journal    
DOI: 10.1002/bab.11     Document Type: Article

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