Epitope mapping of the Brucella melitensis BP26 immunogenic protein: Usefulness for diagnosis of sheep brucellosis

Clinical and Diagnostic Laboratory Immunology

Volumn 10, Issue 4, 2003, Pages 647-651

  Seco Mediavilla, Patricia ^a   Verger, Jean Michel ^c   Grayon, Maggy ^c   Cloeckaert, Axel ^c   Marín, Clara M ^b   Zygmunt, Michel S ^c   Fernández Lago, Luis ^a   Vizcaíno, Nieves ^a  

^a UNIVERSITY OF SALAMANCA   (Spain)

^b Instituto Agroalimentario de Aragón IA2   (Spain)

^c CEMAGREF   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIGEN; BP26 PROTEIN; HYBRID PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRUCELLA ABORTUS; BRUCELLA MELITENSIS; BRUCELLA OVIS; BRUCELLA SUIS; BRUCELLOSIS; CONTROLLED STUDY; EPITOPE MAPPING; GENE SEQUENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RECOMBINANT DNA TECHNOLOGY; SHEEP DISEASE; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; BRUCELLA MELITENSIS; BRUCELLA OVIS; BRUCELLA SUIS; BRUCELLOSIS; EPITOPE MAPPING; GENES, BACTERIAL; IMMUNODOMINANT EPITOPES; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SHEEP; SHEEP DISEASES; SPECIES SPECIFICITY;

EID: 0038105465     PISSN: 1071412X     EISSN: None     Source Type: Journal    
DOI: 10.1128/CDLI.10.4.647-651.2003     Document Type: Article

References (14)

1. Boschiroli, M. L., S. L. Cravero, A. I. Arese, E. Campos, and O. L. Rossetti. 1997. Protection against infection in mice vaccinated with a Brucella abortus mutant. Infect. Immun. 65:798-800.
2. Cloeckaert, A., S. Baucheron, N. Vizcaíno, and M. S. Zygmunt. 2001. Use of recombinant BP26 protein in serological diagnosis of Brucella melitensis infection in sheep. Clin. Diagn. Lab. Immunol. 8:772-775.
3. Cloeckaert, A., H. Salih-Alj Debbarh, N. Vizcaíno, E. Saman, G. Dubray, and M. S. Zygmunt. 1996. Cloning, nucleotide sequence, and expression of the Brucella melitensis bp26 gene coding for a protein immunogenic in infected sheep. FEMS Microbiol. Lett. 140:139-144.
4. Cloeckaert, A., H. Salih-Alj Debbarh, M. S. Zygmunt, and G. Dubray. 1996. Production and characterisation of monoclonal antibodies to Brucella melitensis cytosoluble proteins that are able to differentiate antibody responses of infected sheep from Rev. 1 vaccinated sheep. J. Med. Microbiol. 45:206-213.
5. Cloeckaert, A., J. M. Verger, M. Grayon, M. S. Zygmunt, and O. Grépinet. 1996. Nucleotide sequence and expression of the gene encoding the major 25-kilodalton outer membrane protein of Brucella ovis: evidence for antigenic shift, compared with other Brucella species, due to a deletion in the gene. Infect. Immun. 64:2047-2055.
6. Lindler, L. E., T. L. Hadfield, B. D. Tall, N. J. Snellings, F. A. Rubin, L. L. Van de Verg, D. Hoover, and R. L. Warren. 1996. Cloning of a Brucella melitensis group 3 antigen gene encoding Omp28, a protein recognized by the humoral immune response during human brucellosis. Infect. Immun. 64:2490-2499.
7. Rossetti, O. L., A. I. Arese, M. L. Boschiroli, and S. L. Cravero. 1996. Cloning of Brucella abortus gene and characterization of expressed 26-kilodalton periplasmic protein: potential use for diagnosis. J. Clin. Microbiol. 34:165-169.
8. Salih-Alj Debbarh, H., A. Cloeckaert, G. Bézard, G. Dubray, and M. S. Zygmunt. 1996. Enzyme-linked immunosorbent assay with partially purified cytosoluble 28-kilodalton protein for serological differentiation between Brucella melitensis-infected and B. melitensis Rev. 1-vaccinated sheep. Clin. Diagn. Lab. Immunol. 3:305-308.
9. Salih-Alj Debbarh, H., M. S. Zygmunt, G. Dubray, and A. Cloeckaert. 1996. Competitive enzyme-linked immunosorbent assay using monoclonal antibodies to the Brucella melitensis BP26 protein to evaluate antibody responses in infected and B. melitensis Rev.1 vaccinated sheep. Vet. Microbiol. 53:325-337.
10. Sangari, F. J., J. M. García-Lobo, and J. Agüero. 1994. The Brucella abortus vaccine strain carries a deletion in the erythritol catabolic genes. FEMS Microbiol. Lett. 121:337-342.
11. Thomson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignments through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
12. Vizcaíno, N., A. Cloeckaert, M. S. Zygmunt, and L. Fernández-Lago. 1999. Molecular characterization of a Brucella species large DNA fragment deleted in Brucella abortus strains: evidence for a locus involved in the synthesis of a polysaccharide. Infect. Immun. 67:2700-2712.
13. Vizcaíno, N., R. Kittelberger, A. Cloeckaert, C. M. Marín, and L. Fernández-Lago. 2001. Minor nucleotide substitutions in the omp31 gene of Brucella ovis result in antigenic differences in the major outer membrane protein that it encodes compared to those of the other Brucella species. Infect. Immun. 69:7020-7028.
14. Zygmunt, M. S., S. Baucheron, N. Vizcaíno, R. A. Bowden, and A. Cloeckaert. 2002. Single-step purification and evaluation of recombinant BP26 protein for serological diagnosis of Brucella ovis infection in rams. Vet. Microbiol. 87:213-220.