Improved mycobacterial protein production using a Mycobacterium smegmatis groEL1ΔC expression strain

BMC Biotechnology

Volumn 11, Issue , 2011, Pages

  Noens, Elke E ^a   Williams, Chris ^a   Anandhakrishnan, Madhankumar ^a   Poulsen, Christian ^b   Ehebauer, Matthias T ^a   Wilmanns, Matthias ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY BEADS; C-TERMINUS; CODING SEQUENCES; EXPRESSION SYSTEM; FUNCTIONAL PROTEINS; MYCOBACTERIAL; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS; NON-PATHOGENIC BACTERIUM; POLYHISTIDINE; PROTEIN PRODUCTION; PROTEIN PURIFICATION; PROTEIN SAMPLES; PURIFICATION EFFICIENCY; RECOMBINANT PROTEIN; TARGET PROTEINS;

AMINO ACIDS; BACTERIA; PURIFICATION;

PROTEINS;

HEAT SHOCK PROTEIN 60; HISTIDINE; POLYHISTIDINE; PROTEIN GROEL1; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; BACTERIAL DNA; BACTERIAL PROTEIN; CHAPERONE; CHAPERONIN;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; CARBOXY TERMINAL SEQUENCE; MYCOBACTERIUM SMEGMATIS; NONHUMAN; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; BIOFILM; CIRCULAR DICHROISM; GENETIC ENGINEERING; GENETIC TRANSFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; METHODOLOGY; MUTATION; MYCOBACTERIUM TUBERCULOSIS; PHYSIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; SOUTHERN BLOTTING;

BACTERIA (MICROORGANISMS); MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS;

BACTERIAL PROTEINS; BIOFILMS; BLOTTING, SOUTHERN; CHAPERONIN 60; CIRCULAR DICHROISM; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENETIC ENGINEERING; MOLECULAR CHAPERONES; MUTATION; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS; TRANSFORMATION, GENETIC;

EID: 79953080435     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-11-27     Document Type: Article

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