MOVAS-1 cell line: A new in vitro model of vascular calcification

International Journal of Molecular Medicine

Volumn 27, Issue 5, 2011, Pages 663-668

  Mackenzie, N C W ^a   Zhu, D ^a   Longley, L ^a   Patterson, C S ^a   Kommareddy, S ^a   MacRae, V E ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Mouse; MOVAS 1; Vascular calcification; Vascular smooth muscle cells

Indexed keywords

ALIZARIN; ALKALINE PHOSPHATASE; ASCORBIC ACID; ETIDRONIC ACID; GLYCEROL 2 PHOSPHATE; LEVAMISOLE; MESSENGER RNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BLOOD VESSEL CALCIFICATION; CONTROLLED STUDY; ENZYME ACTIVITY; GENE EXPRESSION; IN VITRO STUDY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PUBLICATION; SMOOTH MUSCLE FIBER;

ALKALINE PHOSPHATASE; ANIMALS; ANTHRAQUINONES; BONE DENSITY CONSERVATION AGENTS; CALCINOSIS; CALCIUM; CELL LINE; COLORING AGENTS; ETIDRONIC ACID; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GLYCEROPHOSPHATES; LEVAMISOLE; MICE; MICE, INBRED C57BL; MUSCLE, SMOOTH, VASCULAR; OSTEOCALCIN; PHOSPHATES; TRANSCRIPTION FACTOR PIT-1; TRANSCRIPTION, GENETIC; VASCULAR DISEASES;

EID: 79952968831     PISSN: 11073756     EISSN: 1791244X     Source Type: Journal    
DOI: 10.3892/ijmm.2011.631     Document Type: Article

References (46)

1. Shroff RC and Shanahan CM: The vascular biology of calcification. Semin Dial 20: 103-109, 2007.
2. Demer LL and Tintut Y: Vascular calcification: pathobiology of a multifaceted disease. Circulation 117: 2938-2948, 2008. (Pubitemid 351787058)
3. Schenker MP, Dorbala S, Hong ECT, Rybicki FJ, Hachamovitch R, Kwong RY and Di Carli MF: Interrelation of coronary calcification, myocardial ischemia, and outcomes in patients with intermediate likelihood of coronary artery disease: a combined positron emission tomography/computed tomography study. Circulation 117: 1693-1700, 2008. (Pubitemid 351482594)
4. Steitz SA, Speer MY, Curinga G, Yang HY, Haynes P, Aebersold R, Schinke T, Karsenty G and Giachelli CM: Smooth muscle cell phenotypic transition associated with calcification: upregulation of Cbfa1 and downregulation of smooth muscle lineage markers. Circ Res 89: 1147-1154, 2001. (Pubitemid 34627872)
5. Abedin M, Tintut Y and Demer LL: Vascular calcification: mechanisms and clinical ramifications. Arterioscler Thromb Vasc Biol 24: 1161-1170, 2004. (Pubitemid 38880003)
6. Yang H, Curinga G and Giachelli CM: Elevated extracellular calcium levels induce smooth muscle cell matrix mineralization in vitro. Kidney Int 66: 2293-2299, 2004. (Pubitemid 41115292)
7. i depletion promotes spontaneous aortic calcification in NPP1-/- mice. Arterioscler Thromb Vasc Biol 25: 686-691, 2005.
8. Speer MY, Chien YC, Quan M, Yang HY, Vali H, McKee MD and Giachelli CM: Smooth muscle cells deficient in osteopontin have enhanced susceptibility to calcification in vitro. Cardiovasc Res 66: 324-333, 2005. (Pubitemid 40471047)
9. Towler DA, Bidder M, Latifi T, Coleman T and Semenkovich CF: Diet-induced diabetes activates an osteogenic gene regulatory program in the aortas of low density lipoprotein receptor-deficient mice. J Biol Chem 273: 30427-30434, 1998. (Pubitemid 28545516)
10. Tintut Y, Alfonso Z, Saini T, Radcliff K, Watson K, Boström K and Demer LL: Multilineage potential of cells from the artery wall. Circulation 108: 2505-2510, 2003. (Pubitemid 37444413)
11. Reynolds JL, Joannides AJ, Skepper JN, McNair R, Schurgers LJ, Proudfoot D, Jahnen-Dechent W, Weissberg PL and Shanahan CM: Human vascular smooth muscle cells undergo vesicle-mediated calcification in response to changes in extracellular calcium and phosphate concentrations: a potential mechanism for accelerated vascular calcification in ESRD. J Am Soc Nephrol 15: 2857-2867, 2004. (Pubitemid 41103395)
12. Alexander MY, Wilkinson FL, Kirton JP, Rock CF, Collett GD, Jeziorska M, Smyth JV, Heagerty AM and Canfield AE: Identification and characterization of vascular calcification-associated factor, a novel gene upregulated during vascular calcification in vitro and in vivo. Arterioscler Thromb Vasc Biol 25: 1851-1857, 2005.
13. Shao JS, Cheng SL, Pingsterhaus JM, Charlton-Kachigian N, Loewy AP and Towler DA: Msx2 promotes cardiovascular calcification by activating paracrine Wnt signals. J Clin Invest 115: 1210-1220, 2005. (Pubitemid 40629039)
14. Murshed M, Harmey D, Millán JL, McKee MD and Karsenty G: Unique coexpression in osteoblasts of broadly expressed genes accounts for the spatial restriction of ECM mineralization to bone. Genes Dev 19: 1093-1104, 2005. (Pubitemid 40632454)
15. Aikawa E, Nahrendorf M, Figueiredo JL, Swirski FK, Shtatland T, Kohler RH, Jaffer FA, Aikawa M and Weissleder R: Osteogenesis associates with inflammation in early-stage atherosclerosis evaluated by molecular imaging in vivo. Circulation 116: 2030-2034, 2008.
16. Shroff RC, McNair R, Figg N, Skepper JN, Schurgers L, Gupta A, Hiorns M, Donald AE, Deanfield J, Rees L and Shanahan CM: Dialysis accelerates medial vascular calcification in part by triggering smooth muscle cell apoptosis. Circulation 118: 1748-1757, 2008.
17. Li X, Yang HY and Giachelli CM: Role of the sodium-dependent phosphate cotransporter, PiT-1, in vascular smooth muscle cell calcification. Circ Res 98: 905-912, 2006.
18. Narisawa S, Harmey D, Yadav MC, O'Neill WC, Hoylaerts MF and Millán JL: Novel inhibitors of alkaline phosphatase suppress vascular smooth muscle cell calcification. J Bone Miner Res 22: 1700-1710, 2007. (Pubitemid 351235136)
19. Lomashvili KA, Garg P, Narisawa S, Millan JL and O'Neill WC: Upregulation of alkaline phosphatase and pyrophosphate hydrolysis: potential mechanism for uremic vascular calcification. Kidney Int 73: 1024-1030, 2008. (Pubitemid 351543906)
20. Byon CH, Javed A, Dai Q, Kappes JC, Clemens TL, Darley-Usmar VM, McDonald JM and Chen Y: Oxidative stress induces vascular calcification through modulation of the osteogenic transcription factor Runx2 by AKT signaling. J Biol Chem 283: 15319-15327, 2008.
21. Huang MS, Sage AP, Lu J, Demer LL and Tintut Y: Phosphate and pyrophosphate mediate PKA-induced vascular cell calcification. Biochem Biophys Res Commun 374: 553-558, 2008.
22. Schurgers LJ, Spronk HM, Skepper JN, Hackeng TM, Shanahan CM, Vermeer C, Weissberg PL and Proudfoot D: Post-translational modifications regulate matrix Gla protein function: importance for inhibition of vascular smooth muscle cell calcification. J Thromb Haemost 5: 2503-2511, 2007. (Pubitemid 350154362)
23. Ewence AE, Bootman M, Roderick HL, Skepper JN, McCarthy G, Epple M, Neumann M, Shanahan CM and Proudfoot D: Calcium phosphate crystals induce cell death in human vascular smooth muscle cells: a potential mechanism in atherosclerotic plaque destabilization. Circ Res 103: E28-E34, 2008.
24. Shioi J, Pangalos MN, Ripellino JA, Vassilacopoulou D, Mytilineou C, Margolis RU and Robakis NK: The Alzheimer amyloid precursor proteoglycan (appican) is present in brain and is produced by astrocytes but not by neurons in primary neural cultures. J Biol Chem 270: 11839-11844, 1995.
25. Collett GD, Sage AP, Kirton JP, Alexander MY, Gilmore AP and Canfield AE: Axl/phosphatidylinositol 3-kinase signaling inhibits mineral deposition by vascular smooth muscle cells. Circ Res 100: 502-509, 2007. (Pubitemid 46673259)
26. Alam MU, Kirton JP, Wilkinson FL, Towers E, Sinha S, Rouhi M, Vizard TN, Sage AP, Martin D, Ward DT, Alexander MY, Riccardi D and Canfield AE: Calcification is associated with loss of functional calcium-sensing receptor in vascular smooth muscle cells. Cardiovasc Res 81: 260-268, 2009.
27. Luo G, Ducy P, McKee MD, Pinero GJ, Loyer E, Behringer RR and Karsenty G: Spontaneous calcification of arteries and cartilage in mice lacking matrix GLA protein. Nature 386: 78-81, 1997. (Pubitemid 27130923)
28. 2+ ATPase-4 in the vascular smooth muscle cell cycle. Am J Physiol Cell Physiol 285: C88-C95, 2003.
29. Chalmers JA, Martino TA, Tata N, Ralph MR, Sole MJ and Belsham DD: Vascular circadian rhythms in a mouse vascular smooth muscle cell line (MOVAS-1). Am J Physiol Regul Integr Comp Physiol 295: R1529-R1538, 2008.
30. Chalmers JA, Lin SY, Martino TA, Arab S, Liu P, Husain M, Sole MJ and Belsham DD: Diurnal profiling of neuroendocrine genes in murine heart, and shift in proopiomelanocortin gene expression with pressure-overload cardiac hypertrophy. J Mol Endocrinol 41: 117-124, 2008.
31. Choi KW, Park HJ, Jung DH, Kim TW, Park YM, Kim BO, Sohn EH, Moon EY, Um SH, Rhee DK and Pyo S: Inhibition of TNF-α-induced adhesion molecule expression by diosgenin in mouse vascular smooth muscle cells via downregulation of the MAPK, Akt and NF-κB signaling pathways. Vascul Pharmacol 53: 273-280, 2010.
32. Johnson KA, Polewski M and Terkeltaub RA: Transglutaminase 2 is central to induction of the arterial calcification program by smooth muscle cells. Circ Res 102: 529-537, 2008. (Pubitemid 351416752)
33. Lomri A, Marie PJ, Tran PV and Hott M: Characterization of endosteal osteoblastic cells isolated from mouse caudal vertebrae. Bone 9: 165-175, 1988.
34. MacRae VE, Davey MG, McTeir L, Narisawa S, Yadav MC, Millan JL and Farquharson C: Inhibition of PHOSPHO1 activity results in impaired skeletal mineralization during limb development of the chick. Bone 46: 1146-1155, 2010.
35. Farquharson C, Lester D, Seawright E, Jefferies D and Houston B: Microtubules are potential regulators of growth-plate chondrocyte differentiation and hypertrophy. Bone 25: 405-412, 1999. (Pubitemid 29424233)
36. Farquharson C, Berry JL, Mawer EB, Seawright E and Whitehead CC: Regulators of chondrocyte differentiation in tibial dyschondroplasia: an in vivo and in vitro study. Bone 17: 279-286, 1995.
37. MacRae VE, Farquharson C and Ahmed SF: The restricted potential for recovery of growth plate chondrogenesis and longitudinal bone growth following exposure to pro-inflammatory cytokines. J Endocrinol 189: 319-328, 2006.
38. MacRae VE, Horvat S, Pells SC, Dale H, Collinson RS, Pitsillides AA, Ahmed SF and Farquharson C: Increased bone mass, altered trabecular architecture and modified growth plate organization in the growing skeleton of SOCS2 deficient mice. J Cell Physiol 218: 276-284, 2009.
39. Van Belle H: Alkaline phosphatase. I. Kinetics and inhibition by levamisole of purified isoenzymes from humans. Clin Chem 22: 972-976, 1976.
40. 32Pi uptake and mineralization by matrix vesicle-enriched fractions and by hydroxyapatite. Bone 6: 307-312, 1985.
41. Ju H, Gros R, You X, Tsang S, Husain M and Rabinovitch M: Conditional and targeted overexpression of vascular chymase causes hypertension in transgenic mice. Proc Natl Acad Sci USA 98: 7469-7474, 2001. (Pubitemid 32567972)
42. You XM, Mungrue IN, Kalair W, Afroze T, Ravi B, Sadi AM, Gros R and Husain M: Conditional expression of a dominant-negative c-Myb in vascular smooth muscle cells inhibits arterial remodeling after injury. Circ Res 92: 314-321, 2003. (Pubitemid 36258554)
43. Gadeau AP, Chaulet H, Daret D, Kockx M, Daniel-Lamazière JM and Desgranges C: Time course of osteopontin, osteocalcin, and osteonectin accumulation and calcification after acute vessel wall injury. J Histochem Cytochem 49: 79-86, 2001. (Pubitemid 32012843)
44. Addison WN, Azari F, Sørensen ES, Kaartinen MT and McKee MD: Pyrophosphate inhibits mineralization of osteoblast cultures by binding to mineral, up-regulating osteopontin, and inhibiting alkaline phosphatase activity. J Biol Chem 282: 15872-15883, 2007. (Pubitemid 47093287)
45. Boskey AL, Doty SB, Kudryashov V, Mayer-Kuckuk P, Roy R and Binderman I: Modulation of extracellular matrix protein phosphorylation alters mineralization in differentiating chick limb-bud mesenchymal cell micromass cultures. Bone 42: 1061-1071, 2008.
46. Shroff RC, McNair R, Skepper JN, Figg N, Schurgers LJ, Deanfield J, Rees L and Shanahan CM: Chronic mineral dysregulation promotes vascular smooth muscle cell adaptation and extracellular matrix calcification. J Am Soc Nephrol 21: 103-112, 2010.