Immunodetection of retinoblastoma-related protein and its phosphorylated form in interphase and mitotic alfalfa cells

Journal of Experimental Botany

Volumn 62, Issue 6, 2011, Pages 2155-2168

  Ábrahám, Edit ^a   Miskolczi, Pál ^a,b   Ayaydin, Ferhan ^a   Yu, Ping ^a   Kotogány, Edit ^a   Bakó, László ^a,b   Ötvös, Krisztina ^a   Horváth, Gábor V ^a   Dudits, Dénes ^a  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^b UMEÅ UNIVERSITY   (Sweden)

Author keywords

Auxin; cell cycle; cell synchronization; colchicine; cyclin dependent kinases; immunolocalization; phosphorylation; prophase; retinoblastoma related protein; roscovitine

Indexed keywords

COLCHICINE; CYCLIN DEPENDENT KINASE; PHYTOHORMONE; PURINE DERIVATIVE; ROSCOVITINE; TUBULIN MODULATOR; VEGETABLE PROTEIN;

ALFALFA; ARTICLE; CELL CULTURE; DRUG ANTAGONISM; IMMUNOHISTOCHEMISTRY; INTERPHASE; METABOLISM; MITOSIS; PHOSPHORYLATION;

CELLS, CULTURED; COLCHICINE; CYCLIN-DEPENDENT KINASES; IMMUNOHISTOCHEMISTRY; INTERPHASE; MEDICAGO SATIVA; MITOSIS; PHOSPHORYLATION; PLANT GROWTH REGULATORS; PLANT PROTEINS; PURINES; TUBULIN MODULATORS;

ARABIDOPSIS; MEDICAGO SATIVA;

EID: 79952822619     PISSN: 00220957     EISSN: 14602431     Source Type: Journal    
DOI: 10.1093/jxb/erq413     Document Type: Article

References (52)

1. Ach RA, Durfee T, Miller AB, Taranto P, Hanley-Bowdoin L, Zambrski P, Gruissem W. 1997. RRB1 and RRB2 encode maize retinoblastoma-related proteins that interact with a plant D-type cyclin and geminivirus replication protein. Molecular and Cellular Biology17, 5077-5086. (Pubitemid 27357606)
2. Berckmans B, De Veylder L. 2009. Transcriptional control of the cell cycle. Current Opinion in Plant Biology 12, 599-605.
3. Bhattacharyya B, Panda D, Gupta S, Banerjee M. 2008. Anti-mitotic activity of colchicine and the structural basis for its interaction with tubulin. Medicinal Research Reviews 28, 155-183. (Pubitemid 350274963)
4. Blom N, Sicheritz-Ponten T, Gupta R, Gammeltoft S, Brunak S. 2004. Prediction of post-translational glycosylation and phosphorylation of proteins from the amino acid sequence. Proteomics 4, 1633-1649. (Pubitemid 38738322)
5. 2-dependent protein kinase from alfalfa (Medicago varia): partial purification and autophosphorylation. Plant Science 58, 135-144.
6. Boniotti MB, Gutierrez C 2001. A cell-cycle-regulated kinase activity phosphorylates plant retinoblastoma protein and contains, in Arabidopsis, a CDKA/cyclin D complex. The Plant Journal 28, 341-350. (Pubitemid 33066398)
7. Borghi L, Gutzat R, Fütterer J, Laizet Y, Hennig L, Gruissem W. 2010. Arabidopsis RETINOBLASTOMA-RELATED is required for stem cell maintenance, cell differentiation, and lateral organ production. The Plant Cell 22, 1792-1811.
8. Boruc J, Mylle E, Duda M, De Clercq R, Rombauts S, Geelen D, Hilson P, Inzé D, Van Damme D, Russinova E. 2010. Systematic localization of the Arabidopsis core cell cycle proteins reveals novel cell division complexes. Plant Physiology 152, 553-565.
9. Chen Z, Hafidh S, Poh SH, Twell D, Berger F. 2009. Proliferation and cell fate establishment during Arabidopsis male gametogenesis depends on the retinoblastoma protein. Proceedings of the National Academy of Sciences, USA 106, 7257-7262.
10. Costa LM, Gutierrez-Marcos JF. 2008. Retinoblastoma makes its mark on imprinting in plants. PLoS Biology 6,1631-1633.
11. de Almeida J, Engler J, De Veylder L, et al. 2009. Systematic analysis of cell-cycle gene expression during Arabidopsis development. The Plant Journal 59, 645-660.
12. de Jager SM, Maughan S, Dewitte W, Scofield S, Murray JA. 2005. The developmental context of cell-cycle control in plants. Seminars in Cell and Developmental Biology 16, 385-396.
13. Dudits D, Cserháti M, Miskolczi P, Horváth VG. 2007. The growing family of plant cyclin-dependent kinases with multiple functions in cellular and developmental regulation. In: Inzé D, ed. Cell cycle control andplant development. Oxfore: Blackwell Publishing, 1-30.
14. Durfee T, Feiler HS, Gruissem W. 2000. Retinoblastoma-related proteins in plants: homologues or orthologues of their metazoan counterparts? Plant Molecular Biology 43, 635-642.
15. Farkas T, Hansen K, Holm K, Lukas J, Bartek J. 2002. Distinct phosphorylation events regulate p130-and p107-mediated repression of E2F-4. Journal of Biological Chemistry 277, 26741-26752. (Pubitemid 34951678)
16. Galbraith DW, Harkins KR, Maddox JM, Ayres NM, Sharma DP, Firoozabady E. 1993. Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220, 1049-1051.
17. Giacinti C, Giordano A. 2006. RB and cell cycle progression; Oncogene 25, 5220-5227. (Pubitemid 44299080)
18. Grafi G, Burnett RJ, Helentjaris T, Larkins BA, DeCaprio JA, Sellers WR, Kaelin WG Jr. 1996. A maize cDNA encoding a member of the retinoblastoma protein family: involvement in endoreduplication. Proceedings of the National Academy of Sciences, USA 93, 8962-8967. (Pubitemid 26282000)
19. Gutierrez C 1998. The retinoblastoma pathway in plant cell cycle and development. Current Opinion in Plant Biology 1, 492-497. (Pubitemid 128654002)
20. Hall TA. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 95-98.
21. Hernando E, Nahle Z, Juan G, et al. 2004. Rb inactivation promotes genomic instability by uncoupling cell cycle progression from mitotic control. Nature 430, 797-802. (Pubitemid 39071352)
22. Hirano H, Harashima H, Shinmyo A, Sekine M. 2008. Arabidopsis retinoblastoma-related protein1 is involved in G1 phase cell cycle arrest caused by sucrose starvation. Plant Molecular Biology 66, 259-275.
23. Horváth BM, Magyar Z, Zhang Y, Hamburger AW, Bakó L, Visser RG, Bachem CW, Bögre L. 2006. EBP1 regulates organ size through cell growth and proliferation in plants. EMBO Journal 25, 4909-4920. (Pubitemid 44607035)
24. Huntley R, Healy S, Freeman D, et al. 1998. The maize retinoblastoma protein homologue ZmRb-1 is regulated during leaf development and displays conserved interactions with G1/S regulators and plant cyclin D (CycD) proteins. Plant Molecular Biology 37, 155-169. (Pubitemid 28219677)
25. Johnston AJ, Kirioukhova O, Barrell PJ, Rutten T, Moore JM, Baskar R, Grossniklaus U, Gruissem W. 2010. Dosage-sensitive function of RETINOBLASTOMA RELATED and convergent epigenetic control are required during the Arabidopsis life cycle. PLoS Genetics 6, e1000988.
26. Karantza V, Maroo A, Fay D, Sedivy JM. 1993. Overproduction of Rb protein after the G1/S boundary causes G2 arrest. Molecular Cell Biology 13, 6640-6652. (Pubitemid 23321118)
27. Kawamura K, Kato K, Shinmyo A, Sekine M. 2006. Tobacco retinoblastoma-related protein is phosphorylated by different types of cyclin-dependent kinases during the cell cycle. Plant Biotechnology 23, 467-473. (Pubitemid 46021029)
28. Korenjak M, Brehm A. 2005. E2F-Rb complexes regulating transcription of genes important for differentiation and development. Current Opinion in Genetics and Development 15, 520-527. (Pubitemid 41262408)
29. Lendvai A, Pettkó-Szandtner A, Csordás-Tóth E, Miskolczi P, Horváth GV, Györgyey J, Dudits D. 2007. Dicot and monocot plants differ in retinoblastoma-related protein subfamilies. Journal of Experimental Botany 58, 1663-75. (Pubitemid 47356006)
30. Longworth MS, Herr A, Ji JY, Dyson NJ. 2008. RBF1 promotes chromatin condensation through a conserved interaction with the Condensin II protein dCAP-D3. Genes and Development 22, 1011-1024. (Pubitemid 351544240)
31. Magyar Z, Bakó L, Bögre L, Dedeoglu D, Kapros T, Dudits D. 1993. Active cdc2 genes and cell cycle phase-specific cdc2-related kinase complexes in hormone-stimulated alfalfa cells. The Plant Journal 4, 151-161.
32. Magyar Z, Mészáros T, Miskolczi P, et al. 1997. Cell cycle phase specificity of putative cyclin-dependent kinase variants in synchronized alfalfa cells. The Plant Cell 9, 223-235. (Pubitemid 27161895)
33. Mészáros T, Miskolczi P, Ayaydin F, Pettkó-Szandtner A, Peres A, Magyar Z, Horváth GV, Bakó L, Fehér A, Dudits D. 2000. Multiple cyclin-dependent kinase complexes and phosphatases control G2/M progression in alfalfa cells. Plant Molecular Biology 43, 595-605.
34. Miskolczi P, Lendvai A, Horváth GV, Pettkó-Szandtner A, Dudits D. 2007. Conserved functions of retinoblastoma proteins: from purple retina to green plant cells. Plant Science 172, 671-683.
35. Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiology 15, 473-497.
36. Nair JS, Ho AL, Tse AN, Coward J, Cheema H, Ambrosini G, Keen N, Schwartz GK. 2009. Aurora B kinase regulates the postmitotic endoreduplication checkpoint via phosphorylation of the retinoblastoma protein at serine 780. Molecular Biology of the Cell 20, 2218-2228.
37. Nakagami H, Kawamura K, Sugisaka K, Sekine M, Shinmyo A. 2002. Phosphorylation of retinoblastoma-related protein by the cyclin D/cyclin-dependent kinase complex is activated at the G1/S-phase transition in tobacco. The Plant Cell 14, 1847-1857. (Pubitemid 35002938)
38. Nakagami H, Sekine M, Murakami H, Shinmyo A. 1999. Tobacco retinoblastoma-related protein phosphorylated by a distinct cyclin-dependent kinase complex with Cdc2/cyclin D in vitro. The Plant Journal 18, 243-252.
39. Nieuwland J, Menges M, Murray JAH. 2007. The plant cyclins. In: Inzé D, ed. Cell cycle control and plant development. Oxford: Blackwell Publishing, 31-62.
40. Nieuwland J, Scofield S, Murray JA. 2009. Control of division and differentiation of plant stem cells and their derivatives. Seminars in Cell and Developmental Biology 20, 1134-1142.
41. Park JA, Ahn JW, Kim YK, Kim J, Kim SJ, Kim JK, Kim WT, Pai HS. 2005. Retinoblastoma protein regulates cell proliferation, differentiation, and endoreduplication in plants. The Plant Journal 42, 153-163. (Pubitemid 40589379)
42. Paz Sanchez M, Caro E, Desvoyes B, Ramirez-Parra E, Gutierrez C 2008. Chromatin dynamics during the plant cell cycle. Seminars in Cell and Developmental Biology 19, 537-546.
43. Planchais S, Glab N, Tréhin C, Perennes C, Bureau JM, Meijer L, Bergounioux C 1997. Roscovitine, a novel cyclin-dependent kinase inhibitor, characterizes restriction point and G2/M transition in tobacco BY-2 cell suspension. The Plant Journal 12, 191-202. (Pubitemid 27312775)
44. Poznic M. 2009. Retinoblastoma protein: a central processing unit. Journal of Biosciences 34, 305-312.
45. Sabelli PA, Larkins BA. 2009. The contribution of cell cycle regulation to endosperm development. Sexual Plant Reproduction 22, 207-219.
46. Sablowski R. 2007. The dynamic plant stem cell niches. Current Opinion in Plant Biology 10, 639-644. (Pubitemid 350030603)
47. Shimizu-Sato S, Ike Y, Mori H. 2008. PsRBR1 encodes a pea retinoblastoma-related protein that is phosphorylated in axillary buds during dormancy-to-growth transition. Plant Molecular Biology 66, 125-135. (Pubitemid 50005024)
48. Soni R, Carmichael JP, Shah ZH, Murray JAH. 1995. Afamily of cyclin D homologs from plants differentially controlled by growth regulators and containing the conserved retinoblastoma protein interaction motif. The Plant Cell 7, 85-103.
49. Sotillo R, Hernando E, Diaz-Rodriguez E, Teruya-Feldstein J, Cordon-Cardo C, Lowe SW, Benezra R. 2007. Mad2 overexpression promotes aneuploidy and tumorigenesis in mice. Cancer Cell 11, 9-23. (Pubitemid 46054519)
50. Wyrzykowska J, Schorderet M, Pien S, Gruissem W, Fleming AJ. 2006. Induction of differentiation in the shoot apical meristem by transient overexpression of a retinoblastoma-related protein. Plant Physiology 141, 1338-1348. (Pubitemid 44223286)
51. Xie Q, Sanz-Burgos AP, Hannon GJ, Gutierrez C 1996. Plant cells contain a novel member of the retinoblastoma family of growth regulatory proteins. EMBO Journal 15, 4900-4908. (Pubitemid 26315800)
52. Zheng L, ChenY Riley DJ, Chen PL, Lee WH. 2000. Retinoblastoma protein enhances the fidelity of chromosome segregation mediated by hsHec1p. Molecular and Cellular Biology 20, 3529-3537. (Pubitemid 30243898)