Engineering of geranylgeranyl pyrophosphate synthase levels and physiological conditions for enhanced carotenoid and astaxanthin synthesis in Xanthophyllomyces dendrorhous

Biotechnology Letters

Volumn 33, Issue 4, 2011, Pages 755-761

  Breitenbach, Jürgen ^a   Visser, Hans ^b,c   Verdoes, Jan C ^b   van Ooyen, Albert J J ^b   Sandmann, Gerhard ^a  

^a GOETHE UNIVERSITY   (Germany)

^b WAGENINGEN UNIVERSITY   (Netherlands)

^c Dyadic Netherlands   (Netherlands)

Author keywords

3 hydroxy 4 keto torulene; Carotenoid biosynthesis; Geranylgeranyl pyrophosphate synthase; Light enhancement

Indexed keywords

ADDITIVE EFFECTS; AIR SUPPLY; ASTAXANTHIN; BIOLOGICAL PRODUCTION; CAROTENOID BIOSYNTHESIS; CAROTENOID SYNTHESIS; DIM LIGHT; GENE PRODUCTS; LIGHT ENHANCEMENT; PHYSIOLOGICAL CONDITION; SYNTHASES; TERPENOIDS; TORULENE; TRANSFORMANTS; WILD TYPES; XANTHOPHYLLOMYCES DENDRORHOUS;

BIOCHEMISTRY; BIOSYNTHESIS; GENES; PHYSIOLOGY;

PIGMENTS;

ASTAXANTHIN; CAROTENOID; PHYTOENE SYNTHASE; XANTHOPHYLL;

ARTICLE; BASIDIOMYCETES; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR CLONING; PROTEIN ENGINEERING; SIGNAL TRANSDUCTION; UPREGULATION;

BASIDIOMYCOTA; CAROTENOIDS; CLONING, MOLECULAR; GERANYLGERANYL-DIPHOSPHATE GERANYLGERANYLTRANSFERASE; PROTEIN ENGINEERING; SIGNAL TRANSDUCTION; UP-REGULATION; XANTHOPHYLLS;

FUNGI; XANTHOPHYLLOMYCES DENDRORHOUS;

EID: 79952763038     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0495-2     Document Type: Article

References (19)

1. Alcaino J, Barahona S, Carmona M, Lozano C, Marcoleta A, Niklitschek M, Sepulveda D, Baeza M, Cifuentes V (2008) Cloning of the cytochrome P450 reductase (crtR) gene and its involvement in the astaxanthin biosynthesis of Xanthophyllomyces dendrorhous. BMC Microbiol 8: 169.
2. An GH, Schuman DB, Johnson EA (1989) Isolation of Phaffia rhodozyma mutants with increased astaxanthin content. Appl Environ Microbiol 55: 116-124.
3. Andrewes AG, Starr MP (1976) (3R, 3′R)-Astaxanthin from the yeast Phaffia rhodozyma. Phytochemistry 15: 1009-1011.
4. Bjerkeng B (2008) Carotenoids in aquaculture: fish and crustaceans. In: Britton G, Liaaen-Jensen S, Pfander H (eds) Carotenoids, vol 4. Birkhäuser Verlag, Basel, CH, pp 237-254.
5. Borovkov AY, Rivkin MI (1997) XcmI-Containing vector for direct cloning of PCR products. BioTech 22: 812-814.
6. Calo P, de Miguel T, Velazquez JB, Villa TG (1995) Mevalonic acid increases trans-astaxanthin and carotenoid biosynthesis in Phaffia rhodozyma. Biotechnol Lett 17: 575-578.
7. de la Fuente JL, Rodríguez-Sáiz M, Schleissner C, Díez B, Peiro E, Barredo JL (2010) High-titer production of astaxanthin by the semi-industrial fermentation of Xanthophyllomyces dendrorhous. J Biotechnol 148: 144-146.
8. Hanna Z, Fregau C, Prefontaine G, Brousseau R (1984) Construction of a family of universal expression plasmid vectors. Gene 30: 247-250.
9. Hussein G, Sankawa U, Goto H, Matsumoto K, Watanabe H (2006) Astaxanthin, a carotenoid with potential in human health and nutrition. J Nat Prod 69: 443-449.
10. Ojima K, Breitenbach J, Visser H, Setoguchi Y, Tabata K, Hoshino T, van den Berg J, Sandmann G (2006) Cloning of the astaxanthin synthase gene from Xanthophyllomyces dendrorhous (Phaffia rhodozyma) and its assignment as a beta-carotene 3-hydroxylase/4-ketolase. Mol Gen Genom 275: 148-158.
11. Sandmann G, Misawa N, Wiedemann W, Vittorioso P, Carattoli A, Morelli G, Macino G (1993) Functional identification of al-3 from Neurospora crassa as the gene for geranylgeranyl pyrophosphate synthase by complementation with crt genes, in vitro characterization of the gene product, and mutant analysis. J Photochem Photobiol B: Biol 18: 245-251.
12. Schroeder WA, Johnson EA (1995) Singlet oxygen and peroxyl radicals regulate carotenoid biosynthesis in Phaffia rhodozyma. J Biol Chem 270: 18374-18379.
13. van Ooijen AJJ, Verdoes JC, Wery J (2007) Recombinant material for carotenoid production. US patent no. 7, 205, 123 B2.
14. Vazquez M, Santos V (1998) 3-Hydroxy-3′, 4′-didehydro-beta-ψ-caroten-4-one (HDCO) from Xanthophyllomyces dendrorhous (Phaffia rhodozyma) cultivated on xylose media. Biotechnol Lett 20: 181-182.
15. Verdoes JC, Krubasik KP, Sandmann G, van Ooyen AJJ (1999a) Isolation and functional characterisation of a novel type of carotenoid biosynthetic gene from Xanthophyllomyces dendrorhous. Mol Gen Genet 262: 453-461.
16. Verdoes JC, Misawa N, van Ooyen AJJ (1999b) Cloning and characterization of the astaxanthin biosynthetic gene encoding phytoene desaturase of Xanthophyllomyces dendrorhous. Biotechnol Bioengin 63: 750-755.
17. Verdoes JC, Sandmann G, Visser H, Diaz M, van Mossel M, van Ooyen AJJ (2003) Metabolic engineering of the carotenoid biosynthetic pathway in the yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma). Appl Environ Microbiol 69: 3728-3738.
18. Verwaal R, Wang J, Meijnen JP, Visser H, Sandmann G, van den Berg JA, van Ooyen AJJ (2007) High level production of beta-carotene in Saccharomyces cerevisiae by successive transformation with carotenogenic genes from Xanthophyllomyces dendrorhous. Appl Environ Microbiol 73: 4342-4350.
19. Visser H, Sandmann G, Verdoes JC (2005) Xantophylls in fungi: metabolic engineering of the astaxanthin biosynthetic pathway in Xantophyllomyces dendrorhous. In: Barredo J (ed) Methods in biotechnology: microbial processes and products. Humana Press Inc., Totowa, NJ, USA, pp 257-272.