XcmI-containing vector for direct cloning of PCR products

BioTechniques

Volumn 22, Issue 5, 1997, Pages 812-814

  Borovkov, Alex Y ^a   Rivkin, Mark I ^a,b  

^a NORTH DAKOTA STATE UNIVERSITY   (United States)

^b ENERGY SYSTEMS INSTITUTE   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CLONING VECTOR; GENE SEQUENCE; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

EID: 0030996054     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97225bm04     Document Type: Article

References (4)

1. Bauer, J., D. Deely, J. Braman, J. Viola and M. Weiner. 1992. pCR-Script SK(+) cloning system: a simple and fast method for PCR cloning. Strateg. Mol. Biol. 5:62-64.
2. Clark, J.M. 1988. Novel-non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases. Nucleic Acids Res. 16:9677-9686.
3. Costa, G.L. and M.P. Weiner. 1994. Polishing with T4 or Pfu polymerase increases the efficiency of cloning of PCR fragments. Nucleic Acids Res. 22:2423.
4. Mead, D.A., N.K. Pey, C. Herrnstadt, R.A. Marcil and L.M. Smith. 1991. A universal method for the direct cloning of PCR amplified nucleic acid. Bio/Technology 9:657-663.