Glioma cells showing IDH1 mutation cannot be propagated in standard cell culture conditions

British Journal of Cancer

Volumn 104, Issue 6, 2011, Pages 968-970

  Piaskowski, S ^a   Bienkowski, M ^a   Stoczynska Fidelus, E ^a   Stawski, R ^a   Sieruta, M ^a   Szybka, M ^a   Papierz, W ^a   Wolanczyk, M ^a   Jaskolski, D J ^a   Liberski, P P ^a   Rieske, P ^a  

^a MEDICAL UNIVERSITY OF LODZ   (Poland)

Author keywords

astrocytic tumours; cell line; EGFR; glioblastoma; IDH1; R132H

Indexed keywords

ARTICLE; ASTROCYTOMA; CANCER CELL CULTURE; CONTROLLED STUDY; GENE; GENE AMPLIFICATION; GENE MUTATION; GENE SEQUENCE; GLIOBLASTOMA; GLIOMA CELL; HETEROZYGOSITY LOSS; HUMAN; HUMAN CELL; HUMAN TISSUE; IDH1 GENE; PRIORITY JOURNAL;

BIOPSY; BRAIN NEOPLASMS; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; DNA MUTATIONAL ANALYSIS; FREEZING; GENES, ERBB-1; GLIOMA; HUMANS; ISOCITRATE DEHYDROGENASE; LOSS OF HETEROZYGOSITY; MUTATION; TISSUE PRESERVATION; TUMOR CELLS, CULTURED;

EID: 79952697258     PISSN: 00070920     EISSN: 15321827     Source Type: Journal    
DOI: 10.1038/bjc.2011.27     Document Type: Article

References (9)

1. Bleeker FE, Lamba S, Leenstra S, Troost D, Hulsebos T, Vandertop WP, Frattini M, Molinari F, Knowles M, Cerrato A, Rodolfo M, Scarpa A, Felicioni L, Buttitta F, Malatesta S, Marchetti A, Bardelli A (2009) IDH1 mutations at residue p.R132 (IDH1(R132)) occur frequently in high-grade gliomas but not in other solid tumours. Hum Mutat 30: 7-11
2. Ducray F, Marie Y, Sanson M (2009) IDH1 and IDH2 mutations in gliomas. N Engl J Med 360: 2248-2249
3. Hartmann C, Meyer J, Balss J, Capper D, Mueller W, Christians A, Felsberg J, Wolter M, Mawrin C, Wick W, Weller M, Herold-Mende C, Unterberg A, Jeuken JW, Wesseling P, Reifenberger G, von Deimling A (2009) Type and frequency of IDH1 and IDH2 mutations are related to astrocytic and oligodendroglial differentiation and age: a study of 1010 diffuse gliomas. Acta Neuropathol 118: 469-474
4. Kloosterhof NK, Bralten LB, Dubbink HJ, French PJ, van den Bent MJ (2011) Isocitrate dehydrogenase-1 mutations: a fundamentally new understanding of diffuse glioma? Lancet Oncol 12: 83-91
5. Pandita A, Aldape KD, Zadeh G, Guha A, James CD (2004) Contrasting in vivo and in vitro fates of glioblastoma cell subpopulations with amplified EGFR. Genes Chromosomes Cancer 39: 29-36 (Pubitemid 37475250)
6. Sanson M, Marie Y, Paris S, Idbaih A, Laffaire J, Ducray F, Hallani SE, Boisselier B, Mokhtari K, Hoang-Xuan K, Delattre JY (2009) Isocitrate dehydrogenase 1 codon 132 mutation is an important prognostic biomarker in gliomas. J Clin Oncol 27: 4150-4154
7. Watanabe T, Nobusawa S, Kleihues P, Ohgaki H (2009) IDH1 mutations are early events in the development of astrocytomas and oligodendro-gliomas. Am J Pathol 174: 1149-1153
8. Witusik-Perkowska M, Rieske P, Hulas-Bigoszewska K, Zakrzewska M, Stawski R, Kulczycka-Wojdala D, Bienkowski M, Stoczynska-Fidelus E, Gresner SM, Piaskowski S, Jaskolski DJ, Papierz W, Zakrzewski K, Kolasa M, Ironside JW, Liberski PP (2010) Glio-blastoma-derived spheroid cultures as an experimental model for analysis of EGFR anomalies. J Neurooncol 2010 (E-pub ahead of print 29 August 2010; doi:10.1007/s11060-010-0352-0)
9. Zhao S, Lin Y, Xu W, Jiang W, Zha Z, Wang P, Yu W, Li Z, Gong L, Peng Y, Ding J, Lei Q, Guan KL, Xiong Y (2009) Glioma-derived mutations in IDH1 dominantly inhibit IDH1 catalytic activity and induce HIF-1alpha. Science 324: 261-265