Whole-blood flow-cytometric analysis of antigen-specific CD4 T-cell cytokine profiles distinguishes active tuberculosis from non-active states

PLoS ONE

Volumn 6, Issue 3, 2011, Pages

  Sester, Urban ^a   Fousse, Mathias ^a   Dirks, Jan ^a   Mack, Ulrich ^a,e   Prasse, Antje ^b   Singh, Mahavir ^c   Lalvani, Ajit ^d   Sester, Martina ^a  

^a SAARLAND UNIVERSITY   (Germany)

^b UNIVERSITY OF FREIBURG   (Germany)

^c LIONEX DIAGNOSTICS AND THERAPEUTICS GMBH   (Germany)

^d NATIONAL HEART AND LUNG INSTITUTE   (United Kingdom)

^e LOVISENBERG DIAKONALE HOSPITAL   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

CD4 ANTIGEN; CD69 ANTIGEN; CULTURE FILTRATE PROTEIN 10; EARLY SECRETORY ANTIGENIC TARGET 6; GAMMA INTERFERON; INTERLEUKIN 2; INTERLEUKIN 25; TUBERCULIN; BACTERIAL ANTIGEN; BACTERIAL PROTEIN; CFP 10 PROTEIN, MYCOBACTERIUM TUBERCULOSIS; CFP-10 PROTEIN, MYCOBACTERIUM TUBERCULOSIS; ESAT 6 PROTEIN, MYCOBACTERIUM TUBERCULOSIS; ESAT-6 PROTEIN, MYCOBACTERIUM TUBERCULOSIS;

ADULT; ARTICLE; BLOOD; BLOOD SAMPLING; CD4+ T LYMPHOCYTE; CLINICAL FEATURE; CONTROLLED STUDY; CYTOKINE RELEASE; FEMALE; FLOW CYTOMETRY; HEALTH STATUS; HUMAN; IMMUNE RESPONSE; MAJOR CLINICAL STUDY; MALE; RECEIVER OPERATING CHARACTERISTIC; SENSITIVITY AND SPECIFICITY; TUBERCULOSIS; DEMOGRAPHY; DIFFERENTIAL DIAGNOSIS; IMMUNOLOGY; MIDDLE AGED;

MYCOBACTERIUM TUBERCULOSIS;

ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; CD4-POSITIVE T-LYMPHOCYTES; DEMOGRAPHY; DIAGNOSIS, DIFFERENTIAL; FEMALE; FLOW CYTOMETRY; HUMANS; INTERFERON-GAMMA; INTERLEUKIN-2; MALE; MIDDLE AGED; TUBERCULOSIS;

EID: 79952677009     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0017813     Document Type: Article

References (26)

1. Lalvani A, (2007) Diagnosing tuberculosis infection in the 21st century: new tools to tackle an old enemy. Chest 131: 1898-1906.
2. Pathan AA, Wilkinson KA, Klenerman P, McShane H, Davidson RN, et al. (2001) Direct Ex Vivo Analysis of Antigen-Specific IFN-gamma-Secreting CD4 T Cells in Mycobacterium tuberculosis-Infected Individuals: Associations with Clinical Disease State and Effect of Treatment. J Immunol 167: 5217-5225.
3. Hinks TS, Dosanjh DP, Innes JA, Pasvol G, Hackforth S, et al. (2009) Frequencies of region of difference 1 antigen-specific but not purified protein derivative-specific gamma interferon-secreting T cells correlate with the presence of tuberculosis disease but do not distinguish recent from remote latent infections. Infect Immun 77: 5486-5495.
4. Sester M, Sotgiu G, Lange C, Giehl C, Girardi E, et al. (2011) Interferon-γ release assays for the diagnosis of active tuberculosis: a systematic review and meta-analysis. Eur Respir J 37: 100-111.
5. Sester U, Presser D, Dirks J, Gärtner BC, Köhler H, et al. (2008) PD-1 Expression and IL-2 Loss of Cytomegalovirus- Specific T Cells Correlates with Viremia and Reversible Functional Anergy. Am J Transplant 8: 1486-1497.
6. Pantaleo G, Harari A, (2006) Functional signatures in antiviral T-cell immunity for monitoring virus-associated diseases. Nat Rev Immunol 6: 417-423.
7. Millington KA, Innes JA, Hackforth S, Hinks TS, Deeks JJ, et al. (2007) Dynamic relationship between IFN-gamma and IL-2 profile of Mycobacterium tuberculosis-specific T cells and antigen load. J Immunol 178: 5217-5226.
8. Mack U, Migliori GB, Sester M, Rieder HL, Ehlers S, et al. (2009) LTBI: latent tuberculosis infection or lasting immune responses to M. tuberculosis? A TBNET consensus statement. Eur Respir J 33: 956-973.
9. Sester M, Sester U, Clauer P, Heine G, Mack U, et al. (2004) Tuberculin skin testing underestimates a high prevalence of latent tuberculosis infection in hemodialysis patients. Kidney Int 65: 1826-1834.
10. Sester U, Junker H, Hodapp T, Schütz A, Thiele B, et al. (2006) Improved efficiency in detecting cellular immunity towards M. tuberculosis in patients receiving immunosuppressive drug therapy. Nephrol Dial Transplant 21: 3258-3268.
11. Dinser R, Fousse M, Sester U, Albrecht K, Singh M, et al. (2008) Evaluation of latent tuberculosis infection in patients with inflammatory arthropathies before treatment with TNF-alpha blocking drugs using a novel flow-cytometric interferon-gamma release assay. Rheumatology (Oxford) 47: 212-218.
12. Sester U, Wilkens H, van Bentum K, Singh M, Sybrecht GW, et al. (2009) Impaired detection of Mycobacterium tuberculosis immunity in patients using high levels of immunosuppressive drugs. Eur Respir J 34: 702-710.
13. Waldrop SL, Davis KA, Maino VC, Picker LJ, (1998) Normal human CD4+ memory T cells display broad heterogeneity in their activation threshold for cytokine synthesis. J Immunol 161: 5284-5295.
14. Sester M, Sester U, Gärtner B, Heine G, Girndt M, et al. (2001) Levels of virus-specific CD4 T cells correlate with cytomegalovirus control and predict virus-induced disease after renal transplantation. Transplantation 71: 1287-1294.
15. Dosanjh DP, Hinks TS, Innes JA, Deeks JJ, Pasvol G, et al. (2008) Improved diagnostic evaluation of suspected tuberculosis. Ann Intern Med 148: 325-336.
16. Biselli R, Mariotti S, Sargentini V, Sauzullo I, Lastilla M, et al. (2010) Detection of interleukin-2 in addition to interferon-gamma discriminates active tuberculosis patients, latently infected individuals, and controls. Clin Microbiol Infect 16: 1282-1284.
17. Sargentini V, Mariotti S, Carrara S, Gagliardi MC, Teloni R, et al. (2009) Cytometric detection of antigen-specific IFN-gamma/IL-2 secreting cells in the diagnosis of tuberculosis. BMC Infect Dis 9: 99.
18. Djoba Siawaya JF, Chegou NN, van den Heuvel MM, Diacon AH, Beyers N, et al. (2009) Differential cytokine/chemokines and KL-6 profiles in patients with different forms of tuberculosis. Cytokine 47: 132-136.
19. Caccamo N, Guggino G, Joosten SA, Gelsomino G, Di Carlo P, et al. (2010) Multifunctional CD4(+) T cells correlate with active Mycobacterium tuberculosis infection. Eur J Immunol 40: 2211-2220.
20. Sutherland JS, Adetifa IM, Hill PC, Adegbola RA, Ota MO, (2009) Pattern and diversity of cytokine production differentiates between Mycobacterium tuberculosis infection and disease. Eur J Immunol 39: 723-729.
21. Casey R, Blumenkrantz D, Millington K, Montamat-Sicotte D, Kon OM, et al. (2010) Enumeration of functional T-cell subsets by fluorescence-immunospot defines signatures of pathogen burden in tuberculosis. PLoS One 5: e15619.
22. Wilkinson KA, Wilkinson RJ, Pathan A, Ewer K, Prakash M, et al. (2005) Ex vivo characterization of early secretory antigenic target 6-specific T cells at sites of active disease in pleural tuberculosis. Clin Infect Dis 40: 184-187.
23. Jafari C, Thijsen S, Sotgiu G, Goletti D, Benitez JA, et al. (2009) Bronchoalveolar lavage enzyme-linked immunospot for a rapid diagnosis of tuberculosis: a Tuberculosis Network European Trialsgroup study. Am J Respir Crit Care Med 180: 666-673.
24. Thomas MM, Hinks TS, Raghuraman S, Ramalingam N, Ernst M, et al. (2008) Rapid diagnosis of Mycobacterium tuberculosis meningitis by enumeration of cerebrospinal fluid antigen-specific T-cells. Int J Tuberc Lung Dis 12: 651-657.
25. Jafari C, Ernst M, Strassburg A, Greinert U, Kalsdorf B, et al. (2008) Local immunodiagnosis of pulmonary tuberculosis by enzyme-linked immunospot. Eur Respir J 31: 261-265.
26. Strassburg A, Jafari C, Ernst M, Lotz W, Lange C, (2008) Rapid diagnosis of pulmonary TB by BAL enzyme-linked immunospot assay in an immunocompromised host. Eur Respir J 31: 1132-1135.