Construction of recombinant lentivirus carrying mouse estrogen receptor α and identification in infected neurons

Academic Journal of Second Military Medical University

Volumn 32, Issue 2, 2011, Pages 160-166

  Hu, Xiao ^a   Wang, Jian Yi ^b   Yuan, Jun ^b   Wan, Xing ^b   Huang, Wei Kun ^b   Qin, Xin Yue ^a  

^a CHONGQING MEDICAL UNIVERSITY   (China)

^b People's Hospital of Guizhou Province   (China)

Author keywords

Estrogen receptor alpha; Neurons; Recombinant lentivirus

Indexed keywords

ESTROGEN RECEPTOR ALPHA; GREEN FLUORESCENT PROTEIN; LENTIVIRUS VECTOR; MESSENGER RNA;

AGAR GEL ELECTROPHORESIS; APOPTOSIS; ARTICLE; BRAIN INFECTION; BRAIN NERVE CELL; DNA SEQUENCE; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; IN VITRO STUDY; NERVE CELL; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

EID: 79952638018     PISSN: 0258879X     EISSN: None     Source Type: Journal    
DOI: 10.3724/SP.J.1008.2011.00160     Document Type: Article

References (29)

1. Vegeto E, Belcredito S, Ghisletti S, Meda C, Etteri S, Maggi A. The endogenous estrogen status regulates microglia reactivity in animal models of neuroinflammation[J]. Endocrinology, 2006,147:2263-2272.
2. Niino M, Hirotani M, Fukazawa T, Kikuchi S, Sasaki H. Estrogens as potential therapeutic agents in multiple sclerosis[J]. Cent Nerv Syst Agents Med Chem, 2009,9:87-94.
3. Heikkinen T, Kalesnykas G, Rissanen A, Tapiola T, Iivonen S, Wang J, et al. Estrogen treatment improves spatial learning in APP + PS1 mice but does not affect beta amyloid accumulation and plaque formation[J]. Exp Neurol, 2004,187:105-117. (Pubitemid 38470017)
4. + T cells and macrophages into the CNS of mice developing experimental encephalomyelitis[J]. Clin Immunol, 2002,102:275-282. (Pubitemid 34259261)
5. Sribnick E A, Wingrave J M, Matzelle D D, Ray S K, Banik N L. Estrogen treatment of spinal cord injury attenuates calpain activation and apoptosis[J]. J neurosci Res, 2006,84:1064-1075.
6. Leranth C, Roth R H, Elsworth J D, Naftolin F, Horvath T L, Redmond D Jr. Estrogen is essential for maintaining nigrostriatal dopamine neurons in primates: implications for Parkinson's disease and memory[J]. J Neurosci, 2000,20:8604-8609.
7. Sierra A, Azcoitia I, Garcia-Segura L. Endogenous estrogen formation is neuroprotective in model of cerebellar ataxia[J]. Endocrine, 2003,21:43-51. (Pubitemid 36677342)
8. Granholm A C, Ford K A, Hyde L A, Bimonte H A, Hunter C L, Nelson M, et al. Estrogen restores cognition and cholinergic phenotype in animal model of Down syndrome[J]. Physiol Behav, 2002,77(2-3):371-385. (Pubitemid 35373893)
9. Pereira M Jr, Soares J M Jr, Valente S G, Oliveira P B, Cavalheiro E A, Amado D, et al. Estrogen effects on pilocarpine-induced temporal lobe epilepsy in rats[J]. Maturitas, 2009,62:190-196.
10. Suzuki S, Brown C M, Dela Cruz C D, Yang E, Bridwell D A, Wise P M. Timing of estrogen therapy after ovariectomy dictates the efficacy of its neuroprotective and antiinflammatory actions[J]. Proc Natl Acad Sci USA, 2007,104:6013-6018. (Pubitemid 47175650)
11. Nilsson S, Mäkelä S, Treuter E, Tujague M, Thomsen J, Andersson G, et al. Mechanisms of estrogen action[J]. Physiol Rev, 2001,81:1535-1565.
12. Hall J M, Couse J F, Korach K S. The multifaceted mechanisms of estradiol and estrogen receptor signaling[J]. J Biol Chem, 2001,276:36869-36872.
13. Leung Y K, Mak P, Hassan S, Ho S M. Estrogen receptor (ER)-beta isoforms:a key to understanding ER-beta signaling [J]. Proc Natl Acad Sci USA, 2006,103:13162-13167. (Pubitemid 44338933)
14. Shang Y, Brown M. Molecular determinants for the tissue specificity of SERMs[J]. Science, 2002,295:2465-2468. (Pubitemid 34270263)
15. Merchenthaler I, Lane M V, Numan S, Dellovade T L. Distribution of estrogen receptor alpha and beta in the mouse central nervous system: in vivo autoradiographic and immunocytochemical analyses[J]. J Comp Neurol, 2004,473:270-291. (Pubitemid 38569232)
16. Morales L B, Loo K K, Liu H B, Peterson C, Tiwari Woodruff S, Voskuhl R R. Treatment with an estrogen receptor alpha ligand is neuroprotective in experimental autoimmune encephalomyelitis[J]. J Neurosci, 2006,26:6823-6833. (Pubitemid 44315243)
17. Kusumawati A, Commes Liautard J P, Widada J S. Transfection of myelomonocytic cell lines: cellular response to a lipid-based reagent and electroporation[J]. Anal Biochem, 1999,1:219-221.
18. Pereboeva L, Komarova S, Mikheeva G, Curiel D T. Approaches to utilize mesenchymal progenitor cells as cellular vehicles [J]. Stem Cells, 2003,21:389-404. (Pubitemid 36793483)
19. Trobridge G, Russell D W. Cell cycle requirements for transduction by foamy virus vectors compared to those of oncovirus and lentivirus vectors[J]. J Virol, 2004,78:2327-2335. (Pubitemid 38228926)
20. Rubinson D A, Dillon C P, Kwiatkowski A V, Sievers C, Yang L, Kopinja J, et al. A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference[J]. Nat Genet, 2003,33:401-406.
21. Wang L F, Goodnead L, Prat C, Mitrophanous K A, Kingsman S M, Mazarakis N D. Lentivirus-mediated gene transfer to the central nervous system: therapeutic and research applications [J]. Hum Gene Ther, 2006,17:1-9. (Pubitemid 43102718)
22. Yip P K, Wong L F, Pattinson D, Battaglia A, Grist J, Bradbury E J, et al. Lentiviral vector expressing retinoic acid receptor beta2 promotes recovery of function after corticospinal tract injury in the adult rat spinal cord[J]. Hum Mol Genet, 2006,15:3107-3118. (Pubitemid 44605493)
23. Philippe S, Sarkis C, Barkats M, Mammeri H, Ladroue C, Petit C, et al. Lentiviral vectors with a defective integrase allow efficient and sustained transgene expression in vitro and in vivo [J]. Proc Natl Acad Sci, 2006,103:17684-17689. (Pubitemid 44852220)
24. Vigna E, Naldini L. Lentiviral vectors: excellent tools for experimental gene transfer and promising candidates for gene therapy [J]. Gene Med, 2000,2:308-316.
25. Mural R J, Adams M D, Myers E W, Smith H O, Miklos G L, Wides R, et al. A comparison of whole-genome shotgun-derived mouse chromosome 16 and the human genome[J]. Science, 2002,296:1661-1671. (Pubitemid 34579160)
26. Zhang Y Z, Wang H S, Pan H, Bao X H, Li M R, Jin J, et al Gene delivery into primary cerebral cortical neurons by lentiviral vector[J]. Cell Biol, 2006,30:777-783. (Pubitemid 44573857)
27. Blomer U, Naldini L, Kafri T, Trono D, Verma I M, Gage F H. Highly efficient and sustained gene transfer in adult neurons with a lentivirus vector[J]. J Virol, 1997,71:6641-6649. (Pubitemid 27355322)
28. Hu X, Lou L, Yuan J, Wan X, Wang J , Qin X. Use of adenovirus vector expressing the mouse full estrogen receptor α gene to infect mouse primary neurons[J]. Neural Regan Res, 2010,5: 1138-1143.