Micropatterns of cell adhesive proteins with poly(ethylene oxide)-block-Poly(4-vinylpyridine) diblock copolymer

Biotechnology and Bioengineering

Volumn 108, Issue 4, 2011, Pages 983-987

  Racine, J ^a   Cheradame, H ^b   Chu, Y S ^c   Thiery, J P ^c   Rodriguez, I ^a  

^a INSTITUTE OF MATERIALS RESEARCH AND ENGINEERING   (Singapore)

^b UNIVERSITÉ D EVRY VAL D ESSONNE   (France)

^c INSTITUTE OF MOLECULAR AND CELL BIOLOGY   (Singapore)

Author keywords

Cell adhesion; Cell micropatterning; PEO b P4VP; Poly(ethylene oxide) poly(4 vinylpyridine)

Indexed keywords

ADHESIVE PROTEINS; ANTIFOULING PROPERTY; APOPTOSIS; CELL BIOLOGY; CELL CHIPS; CELL DIFFERENTIATION; CELL MICROPATTERNING; CELL SHAPES; CELL-ADHESIVE PROTEINS; CROSSLINKS; DIBLOCK COPOLYMER; ETHYLENE OXIDES; MICRO PATTERN; MICROPATTERNED; MICROPATTERNS; PEO-B-P4VP; PHOTOLITHOGRAPHIC MASKS; PHOTOSENSITIVE MATERIALS; POLY(4-VINYL PYRIDINE); POLY(ETHYLENE OXIDE)-POLY(4-VINYLPYRIDINE); SHORT WAVELENGTHS; UV LIGHT;

ADHESION; BLOCK COPOLYMERS; CELL ADHESION; CELL DEATH; ETHYLENE; GROWTH KINETICS; POLYETHYLENE OXIDES; PROTEINS;

CELL IMMOBILIZATION;

4 VINYLPYRIDINE; ANTIFOULING AGENT; CELL ADHESIVE PROTEIN; CELL PROTEIN; COPOLYMER; MACROGOL; PHOTOSENSITIZING AGENT; UNCLASSIFIED DRUG;

CELL ACTIVITY; CELL MICROPATTERNING; CROSS LINKING; DISSOCIATION; FLUORESCENCE MICROSCOPY; IMMOBILIZED CELL; LETTER; PHOTOLITHOTROPH; ULTRAVIOLET RADIATION;

ANIMALS; CELL ADHESION; CELL LINE; FIBRONECTINS; HUMANS; POLYETHYLENE GLYCOLS; SURFACE PROPERTIES; VINYL COMPOUNDS;

EID: 79952601073     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22997     Document Type: Letter

References (16)

1. Ashton RS, Peltier J, Fasano CA, O'Neill A, Leonard J, Temple S, Schaffer DV, Kane RS. 2007. High-throughput screening of gene function in stem cells using clonal microarrays. Stem Cells 25:2928-2935.
2. Azioune A, Storch M, Bornens M, Théry M, Piel M. 2009. Simple and rapid process for single cell micro-patterning. Lab Chip 9:1640-1642.
3. Chen CS, Mrksich M, Huang S, Whitesides GM, Ingber DE. 1997. Geometric control of cell life and death. Science 276:1425-1428.
4. Chu YS, Thomas WA, Eder O, Pincet F, Perez E, Thiery JP, Dufour S. 2004. Force measurements in E-cadherin-mediated cell doublets reveal rapid adhesion strengthened by actin cytoskeleton remodeling through Rac and Cdc42. J Cell Biol 167:1183-1194.
5. Falconnet D, Koenig A, Assi F, Textor M. 2004. A combined photolithographic and molecular-assembly approach to produce functional micropatterns for applications in the biosciences. Adv Funct Mater 14:749-756.
6. Falconnet D, Csucs G, Grandin HM, Textor M. 2006. Surface engineering approaches to micropattern surfaces for cell-based assays. Biomaterials 27:3044-3063.
7. Fink J, Théry M, Azioune A, Dupont R, Chatelain F, Bornens M, Piel M. 2007. Comparative study and improvement of current cell micro-patterning techniques. Lab Chip 7:672-680.
8. Geiger B, Spatz JP, Bershadsky AD. 2009. Environmental sensing through focal adhesions. Nat Rev Mol Cell Biol 10:21-33.
9. Grozea CM, Gunari N, Finlay JA, Grozea D, Callow ME, Callow JA, Lu ZH, Walker GC. 2009. Water-stable diblock polystyrene-block-poly(2-vinyl pyridine) and diblock polystyrene-block-poly(methyl methacrylate) cylindrical patterned surfaces inhibit settlement of zoospores of the green alga Ulva. Biomacromolecules 10:1004-1012.
10. Kane RS, Takayama S, Ostuni E, Ingber DE, Whitesides GM. 1999. Patterning proteins and cells using soft lithography. Biomaterials 20:2363-2376.
11. Kilian KA, Bugarija B, Lahn BT, Mrksich M. 2010. Geometric cues for directing the differentiation of mesenchymal stem cells. PNAS 107:4872-4877.
12. Liu H, Shi RH, Wan WM, Yang RM, Wang YM. 2008. A well-defined diblock copolymer of poly(ethylene oxide)-block-poly(4-vinylpyridine) for separation of basic proteins by capillary zone electrophoresis. Electrophoresis 29:2812-2819.
13. Théry M, Racine V, Pépin A, Piel1 M, Chen Y, Sibarita JB, Bornens M. 2005. The extracellular matrix guides the orientation of the cell division. Nat Cell Biol 7:947-953.
14. Théry M, Racine V, Piel M, Pépin A, Dimitrov A, Chen Y, Sibarita JB, Bornens M. 2006. Directing cell migration with asymmetric micropatterns. PNAS 103:19771-19776.
15. Weghuber J, Sunzenauer S, Brameshuber M, Plochberger B, Hesch C, Schutz GJ. 2010. In-vivo detection of protein-protein interactions on micro-patterned surfaces. J Vis Exp 37(pii): 1969. DOI: 10.3791/1969.
16. Zhao WA, Teo GSL, Kumar N, Karp JM. 2010. Chemistry and material science at the cell surface. Mater Today 13:14-1421.