In-vivo detection of protein-protein interactions on micro-patterned surfaces

Journal of Visualized Experiments

Volumn , Issue 37, 2010, Pages

  Weghuber, Julian ^a   Sunzenauer, Stefan ^a   Brameshuber, Mario ^a   Plochberger, Birgit ^a   Hesch, Clemens ^a   Schutz, Gerhard J ^a  

^a JOHANNES KEPLER UNIVERSITY LINZ   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80055106830     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1969     Document Type: Article

References (5)

1. Brameshuber, M., Schwarzenbacher, M., Kaltenbrunner, M., Hesch, C., Paster, W., Weghuber, J., Heise, B., Sonnleitner, A., Stockinger, H. and Schuetz, G.J., 2009. Reply to Uncoupling diffusion and binding in FRAP experiments. Nat. Methods. 6, 183-184.
2. Kim, P.W., Sun, Z.J, Blacklow, S.C., Wagner, G., Eck, M.J. A Zinc Clasp Structure Tethers Lck to T Cell Coreceptors CD4 and CD8. Science 301, 1725-1728.
3. Ramm, P., 2005. Image-based screening: a technology in transition. Curr. Opin. Biotechnol. 16, 41-48.
4. Schwarzenbacher, M., Kaltenbrunner, M., Brameshuber, M., Hesch, C., Paster, W., Weghuber, J., Heise, B., Sonnleitner, A., Stockinger, H. and Schutz, G.J., 2008. Micropatterning for quantitative analysis of protein-protein interactions in living cells. Nat. Methods. 5, 1053-1060.
5. Weghuber, J., Brameshuber, M., Sunzenauer, S., Lehner, M., Paar, C., Haselbruebler, T., Schwarzenbacher, M., Kaltenbrunner, M., Hesch, C., Paster, W., Heise, B., Sonnleitner, A., Stockinger, H., and Schutz, G.J. Detection of protein-protein interactions in the live cell plasma membrane by quantifying prey redistribution upon bait micropatterning. Methods in Enzymology, in press.