Images of photoreceptors in living primate eyes using adaptive optics two-photon ophthalmoscopy

Biomedical Optics Express

Volumn 2, Issue 1, 2011, Pages 139-148

  Hunter, Jennifer J ^a,c   Masella, Benjamin ^b,c   Dubra, Alfredo ^a,c   Sharma, Robin ^b,c   Yin, Lu ^c   Merigan, William H ^a,c   Palczewska, Grazyna ^d   Palczewski, Krzysztof ^d,e   Williams, David R ^a,b,c  

^a UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

^b UNIVERSITY OF ROCHESTER   (United States)

^c UNIVERSITY OF ROCHESTER   (United States)

^d Polgenix Inc   (United States)

^e CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EYE MOVEMENTS; FLUORESCENCE; PHOTONS;

EX-VIVO; FUNCTIONAL IMAGING; IN-VIVO; INTRINSIC FLUORESCENCE; LIGHT LEVEL; LIGHT STIMULATION; NUMERICAL APERTURE; OPTICS OF THE EYE; SCANNING LASER OPHTHALMOSCOPE; TWO PHOTON; TWO PHOTON IMAGING; TWO-PHOTON EXCITED FLUORESCENCE;

FLUOROPHORES;

EID: 79952595306     PISSN: None     EISSN: 21567085     Source Type: Journal    
DOI: 10.1364/BOE.2.000139     Document Type: Article

References (31)

1. T. D. Lamb, and E. N. Pugh, Jr., "Dark adaptation and the retinoid cycle of vision, " Prog. Retin. Eye Res. 23(3), 307-380 (2004).
2. G. H. Travis, M. Golczak, A. R. Moise, and K. Palczewski, "Diseases caused by defects in the visual cycle: retinoids as potential therapeutic agents, " Annu. Rev. Pharmacol. Toxicol. 47(1), 469-512 (2007).
3. D. C. Gray, W. Merigan, J. I. Wolfing, B. P. Gee, J. Porter, A. Dubra, T. H. Twietmeyer, K. Ahamd, R. Tumbar, F. Reinholz, and D. R. Williams, "In vivo fluorescence imaging of primate retinal ganglion cells and retinal pigment epithelial cells, " Opt. Express 14(16), 7144-7158 (2006).
4. J. I. Morgan, J. J. Hunter, B. Masella, R. Wolfe, D. C. Gray, W. H. Merigan, F. C. Delori, and D. R. Williams, "Light-induced retinal changes observed with high-resolution autofluorescence imaging of the retinal pigment epithelium, " Invest. Ophthalmol. Vis. Sci. 49(8), 3715-3729 (2008).
5. J. I. Morgan, A. Dubra, R. Wolfe, W. H. Merigan, and D. R. Williams, "In vivo autofluorescence imaging of the human and macaque retinal pigment epithelial cell mosaic, " Invest. Ophthalmol. Vis. Sci. 50(3), 1350-1359 (2008).
6. C. Chen, E. Tsina, M. C. Cornwall, R. K. Crouch, S. Vijayaraghavan, and Y. Koutalos, "Reduction of all-trans retinal to all-trans retinol in the outer segments of frog and mouse rod photoreceptors, " Biophys. J. 88(3), 2278-2287 (2005).
7. J. Dillon, L. Zheng, J. C. Merriam, and E. R. Gaillard, "Transmission spectra of light to the mammalian retina, " Photochem. Photobiol. 71(2), 225-229 (2000).
8. A. Dubra and Z. Harvey, "Registration of 2D images from fast scanning ophthalmic instruments" presented at WBIR 2010-Workshop on Biomedical Image Registration, July 11-13 2010, Lübeck, Germany.
9. G. Wyszecki, and W. S. Stiles, Color Science: Concepts and Methods, Quantitative Data and Formulae (John Wiley & Sons, Inc., 1982).
10. Y. Imanishi, M. L. Batten, D. W. Piston, W. Baehr, and K. Palczewski, "Noninvasive two-photon imaging reveals retinyl ester storage structures in the eye, " J. Cell Biol. 164(3), 373-383 (2004).
11. M. Han, A. Bindewald-Wittich, F. G. Holz, G. Giese, M. H. Niemz, S. Snyder, H. Sun, J. Yu, M. Agopov, O. La Schiazza, and J. F. Bille, "Two-photon excited autofluorescence imaging of human retinal pigment epithelial cells, " J. Biomed. Opt. 11(1), 010501 (2006).
12. A. Bindewald-Wittich, M. Han, S. Schmitz-Valckenberg, S. R. Snyder, G. Giese, J. F. Bille, and F. G. Holz, "Two-photon-excited fluorescence imaging of human RPE cells with a femtosecond Ti:sapphire laser, " Invest. Ophthalmol. Vis. Sci. 47(10), 4553-4557 (2006).
13. M. Han, G. Giese, S. Schmitz-Valckenberg, A. Bindewald-Wittich, F. G. Holz, J. Yu, J. F. Bille, and M. H. Niemz, "Age-related structural abnormalities in the human retina-choroid complex revealed by two-photon excited autofluorescence imaging, " J. Biomed. Opt. 12(2), 024012 (2007).
14. O. La Schiazza, and J. F. Bille, "High-speed two-photon excited autofluorescence imaging of ex vivo human retinal pigment epithelial cells toward age-related macular degeneration diagnostic, " J. Biomed. Opt. 13(6), 064008 (2008).
15. E. J. Gualda, J. M. Bueno, and P. Artal, "Wavefront optimized nonlinear microscopy of ex vivo human retinas, " J. Biomed. Opt. 15(2), 026007 (2010).
16. L. Zhao, J. Qu, and H. Niu, "Identification of endogenous fluorophores in the photoreceptors using autofluorescence spectroscopy, " in Optics in Health Care and Biomedical Optics III, X. Li, Q. Luo, and Y. Gu, eds. (SPIE, 2007).
17. R. C. Sears, and M. W. Kaplan, "Axial diffusion of retinol in isolated frog rod outer segments following substantial bleaches of visual pigment, " Vision Res. 29(11), 1485-1492 (1989).
18. J. S. Wang, and V. J. Kefalov, "An alternative pathway mediates the mouse and human cone visual cycle, " Curr. Biol. 19(19), 1665-1669 (2009).
19. W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, "Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation, " Proc. Natl. Acad. Sci. U.S.A. 100(12), 7075-7080 (2003).
20. S. Huang, A. A. Heikal, and W. W. Webb, "Two-photon fluorescence spectroscopy and microscopy of NAD(P)H and flavoprotein, " Biophys. J. 82(5), 2811-2825 (2002).
21. F. C. Delori, C. K. Dorey, G. Staurenghi, O. Arend, D. G. Goger, and J. J. Weiter, "In vivo fluorescence of the ocular fundus exhibits retinal pigment epithelium lipofuscin characteristics, " Invest. Ophthalmol. Vis. Sci. 36(3), 718-729 (1995).
22. American National Standards Institute, American National Standard for Safe Use of Lasers (American National Standards Institute, Laser Institute of America, 2007).
23. F. C. Delori, R. H. Webb, D. H. Sliney; American National Standards Institute, "Maximum permissible exposures for ocular safety (ANSI 2000), with emphasis on ophthalmic devices, " J. Opt. Soc. Am. A 24(5), 1250-1265 (2007).
24. W. T. Ham, Jr., H. A. Mueller, J. J. Ruffolo, Jr., and A. M. Clarke, "Sensitivity of the retina to radiation damage as a function of wavelength, " Photochem. Photobiol. 29(4), 735-743 (1979).
25. D. Ts'o, J. Schallek, Y. Kwon, R. Kardon, M. Abramoff, and P. Soliz, "Noninvasive functional imaging of the retina reveals outer retinal and hemodynamic intrinsic optical signal origins, " Jpn. J. Ophthalmol. 53(4), 334-344 (2009).
26. K. Tsunoda, G. Hanazono, K. Inomata, Y. Kazato, W. Suzuki, and M. Tanifuji, "Origins of retinal intrinsic signals: a series of experiments on retinas of macaque monkeys, " Jpn. J. Ophthalmol. 53(4), 297-314 (2009).
27. V. J. Srinivasan, Y. Chen, J. S. Duker, and J. G. Fujimoto, "In vivo functional imaging of intrinsic scattering changes in the human retina with high-speed ultrahigh resolution OCT, " Opt. Express 17(5), 3861-3877 (2009).
28. K. Grieve, and A. Roorda, "Intrinsic signals from human cone photoreceptors, " Invest. Ophthalmol. Vis. Sci. 49(2), 713-719 (2008).
29. R. S. Jonnal, J. Rha, Y. Zhang, B. Cense, W. Gao, and D. T. Miller, "In vivo functional imaging of human cone photoreceptors, " Opt. Express 15(24), 16141-16160 (2007).
30. P. Y. Man, D. M. Turnbull, and P. F. Chinnery, "Leber hereditary optic neuropathy, " J. Med. Genet. 39(3), 162-169 (2002).
31. I. J. Holt, A. E. Harding, R. K. Petty, and J. A. Morgan-Hughes, "A new mitochondrial disease associated with mitochondrial DNA heteroplasmy, " Am. J. Hum. Genet. 46(3), 428-433 (1990).