Analysis of an artificially selected GS-NS0 variant with increased resistance to apoptosis

Biotechnology and Bioengineering

Volumn 108, Issue 4, 2011, Pages 880-892

  Browne, Susan M ^a   Al Rubeai, Mohamed ^a  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

Author keywords

Apoptosis; Artificial selection; Autophagy; Cell culture; NS0; Transcriptomic

Indexed keywords

APOPTOSIS; ARTIFICIAL SELECTION; AUTOPHAGY; NS0; TRANSCRIPTOMIC;

ANTIBODIES; BATCH CELL CULTURE; CELL DEATH; CLONE CELLS; FEATURE EXTRACTION; GENE EXPRESSION; PRODUCTIVITY; SIGNALING;

CELL CULTURE;

ACTIVATING TRANSCRIPTION FACTOR; ACTIVATING TRANSCRIPTION FACTOR 5; BCL2 ASSOCIATED ATHANOGENE 3; BCL2 ASSOCIATED ATHANOGENE 3 PROTEIN; CASPASE 12; CASPASE 3; CASPASE 7; CASPASE RECRUITMENT DOMAIN PROTEIN 10; CASPASE RECRUITMENT DOMAIN PROTEIN 15; CD70 ANTIGEN; DEATH RECEPTOR; FAS LIGAND; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INTERLEUKIN 10; LACTIC ACID; LIM HOMEOBOX PROTEIN 4; LYMPHOTOXIN BETA RECEPTOR; MONOCLONAL ANTIBODY; NON METASTATIC CELL 5 PROTEIN; NUCLEOTIDE BINDING OLIGOMERIZATION DOMAIN CONTAINING 1 PROTEIN; PROTEIN; PROTEIN BCL 2; PROTEIN P105; PROTEIN P53; RECOMBINANT ANTIBODY; TSC22 DOMAIN FAMILY 3 PROTEIN; TUMOR NECROSIS FACTOR; TUMOR NECROSIS FACTOR ALPHA; TUMOR NECROSIS FACTOR RECEPTOR; TUMOR NECROSIS FACTOR RECEPTOR ASSOCIATED FACTOR 2; UNCLASSIFIED DRUG;

ANTIBODY PRODUCTION; APOPTOSIS; ARTICLE; ARTIFICIAL SELECTION; AUTOPHAGY; CANCER CELL CULTURE; CELL DEATH; CELL LINE NS06A1; CELL METABOLISM; CELL STRESS; CELL SURVIVAL; CELL VIABILITY; CHEMICAL STRESS; CONTROLLED STUDY; ENDOPLASMIC RETICULUM STRESS; ENVIRONMENTAL STRESS; GENE EXPRESSION; MYELOMA CELL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REPEAT PROCEDURE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; TRANSCRIPTOMICS; UNFOLDED PROTEIN RESPONSE;

ANIMALS; ANTIBODIES, MONOCLONAL; APOPTOSIS; AUTOPHAGY; CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; FAS LIGAND PROTEIN; GENE EXPRESSION; GENE EXPRESSION PROFILING; HUMANS; MICE; RECOMBINANT PROTEINS;

EID: 79952580624     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22994     Document Type: Article

References (58)

1. Abdelrahim M, Liu SX, Safe S. 2005. Induction of endoplasmic reticulum-induced stress genes in Panc-1 pancreatic cancer cells is dependent on Sp proteins. J Biol Chem 280:16508-16513.
2. Altamirano C, Illanes A, Becerra S, Cairo JJ, Godia F. 2006. Considerations on the lactate consumption by CHO cells in the presence of galactose. J Biotechnol 125:547-556.
3. Barnes LM, Moy N, Dickson AJ. 2006. Phenotypic variation during cloning procedures: Analysis of the growth behavior of clonal cell lines. Biotechnol Bioeng 94:530-537.
4. Bergamaschi D, Samuels Y, Jin BQ, Duraisingham S, Crook T, Lu X. 2004. ASPP1 and ASPP2: Common activators of p53 family members. Mol Cell Biol 24:1341-1350.
5. Berrebi D, Bruscoli S, Cohen N, Foussat A, Migliorati G, Bouchet-Delbos L, Maillot MC, Portier A, Couderc J, Galanaud P, Peuchmaur M, Riccardi C, Emilie D. 2003. Synthesis of glucocorticoid-induced leucine zipper (GILZ) by macrophages: An anti-inflammatory and immunosuppressive mechanism shared by glucocorticoids and IL-10. Blood 101:729-738.
6. Bertrand MJM, Doiron K, Labbe K, Korneluk RG, Barker PA, Saleh M. 2009. Cellular inhibitors of apoptosis cIAP1 and cIAP2 are required for innate immunity signaling by the pattern recognition receptors NOD1 and NOD2. Immunity 30:789-801.
7. Birch JR, Boraston RC, Metcalfe H, Brown ME, Bebbington CR, Field RP. 1994. Selecting and designing cell-lines for improved physiological-characteristics. Cytotechnology 15:11-16.
8. Burky JE, Wesson MC, Young A, Farnsworth S, Dionne B, Zhu Y, Hartman TE, Qu LM, Zhou WC, Saner PW. 2007. Protein-free fed-batch culture of non-GS NS0 cell lines for production of recombinant antibodies. Biotechnol Bioeng 96:281-293.
9. Chang DW, Xing Z, Pan Y, Algeciras-Schimnich A, Barnhart BC, Yaish-Ohad S, Peter ME, Yang XL. 2002. c-FLIPL is a dual function regulator for caspase-8 activation and CD95-mediated apoptosis. EMBO J 21:3704-3714.
10. Charbonneau JR, Gauthier ER. 2000. Prolongation of murine hybridoma cell survival in stationary batch culture by Bcl-xL expression. Cytotechnology 34:131-139.
11. Correia JD, Miranda Y, Leonard N, Hsu J, Ulevitch RJ. 2007. Regulation of Nod1-mediated signaling pathways. Cell Death Differ 14:830-839.
12. D'Adamio F, Zollo O, Moraca R, Ayroldi E, Bruscoli S, Bartoli A, Cannarile L, Migliorati G, Riccardi C. 1997. A new dexamethasone-induced gene of the leucine zipper family protects T lymphocytes from TCR/CD3-activated cell death. Immunity 7:803-812.
13. Delfino DV, Agostini M, Spinicelli S, Vacca C, Riccardi C. 2006. Inhibited cell death, NF-kappa B activity and increased IL-10 in TCR-triggered thymocytes of transgenic mice overexpressing the glucocorticoid-induced protein GILZ. Int Immunopharmacol 6:1126-1134.
14. Delfino DV, Agostini M, Spinicelli S, Vito P, Riccardi C. 2004. Decrease of Bcl-xL and augmentation of thymocyte apoptosis in GILZ overexpressing transgenic mice. Blood 104:4134-4141.
15. Dhawan P, Su YJ, Thu YM, Yu YC, Baugher P, Ellis DL, Sobolik-Delmaire T, Kelley M, Cheung TC, Ware CF, Richmond A. 2008. The lymphotoxin-beta receptor is an upstream activator of NF-kappa B-mediated transcription in melanoma cells. J Biol Chem 283:15399-15408.
16. Dhingra S, Sharma AK, Arora RC, Slezak J, Singal PK. 2009. IL-10 attenuates TNF-alpha-induced NF kappa B pathway activation and cardiomyocyte apoptosis. Cardiovasc Res 82:59-66.
17. Dorai H, Kyung YS, Ellis D, Kinney C, Lin CB, Jan D, Moore G, Betenbaugh MJ. 2009. Expression of anti-apoptosis genes alters lactate metabolism of Chinese hamster ovary cells in culture. Biotechnol Bioeng 103:592-608.
18. Festjens N, Vanden Berghe T, Cornelis S, Vandenabeele P. 2007. RIP1, a kinase on the crossroads of a cell's decision to live or die. Cell Death Differ 14:400-410.
19. Gravestein LA, Amsen D, Boes M, Calvo CR, Kruisbeek AM, Borst J. 1998. The TNF receptor family member CD27 signals to Jun N-terminal kinase via Traf-2. Eur J Immunol 28:2208-2216.
20. Han YK, Kim YG, Kim JY, Lee GM. 2010. Hyperosmotic stress induces autophagy and apoptosis in recombinant chinese hamster ovary cell culture. Biotechnol Bioeng 105:1187-1192.
21. Hartman TE, Sar N, Genereux K, Barritt DS, He YM, Burky JE, Wesson MC, Tso JY, Tsurushita N, Zhou WC, Sauer PW. 2007. Derivation and characterization of cholesterol-independent non-GS NS0 cell lines for production of recombinant antibodies. Biotechnol Bioeng 96:294-306.
22. Hwang SO, Lee GM. 2008. Nutrient deprivation induces autophagy as well as apoptosis in Chinese hamster ovary cell culture. Biotechnol Bioeng 99:678-685.
23. Hwang KC, Ok DW, Hong JC, Kim MO, Kim JH. 2003. Cloning, sequencing, and characterization of the murine nm23-M5 gene during mouse spermatogenesis and spermiogenesis. Biochem Biophys Res Commun 306:198-207.
24. Kaneko M, Niinuma Y, Nomura Y. 2003. Activation signal of nuclear factor-kappa B in response to endoplasmic reticulum stress is transduced via IRE1 and tumor necrosis factor receptor-associated factor 2. Biol Pharm Bull 26:931-935.
25. Katz C, Benyamini H, Rotem S, Lebendiker M, Danieli T, Iosub A, Refaely H, Dines M, Bronner V, Bravman T, Shalev DE, Rudiger S, Friedler A. 2008. Molecular basis of the interaction between the antiapoptotic Bcl-2 family proteins and the proapoptotic protein ASPP2. Proc Natl Acad Sci USA 105:12277-12282.
26. Kim NS, Kim SJ, Lee GM. 1998. Clonal variability within dihydrofolate reductase-mediated gene amplified Chinese hamster ovary cells: Stability in the absence of selective pressure. Biotechnol Bioeng 60:679-688.
27. Kubli DA, Ycaza JE, Gustafsson AB. 2007. Bnip3 mediates mitochondrial dysfunction and cell death through Bax and Bak. Biochem J 405:407-415.
28. Lee JH, Takahashi T, Yasuhara N, Inazawa J, Kamada S, Tsujimoto Y. 1999. Bis, a Bcl-2-binding protein that synergizes with Bcl-2 in preventing cell death. Oncogene 18:6183-6190.
29. Lim SF, Chuan KH, Liu S, Loh SOH, Chung BYF, Ong CC, Song Z. 2006. RNAi suppression of Bax and Bak enhances viability in fed-batch cultures of CHO cells. Metab Eng 8:509-522.
30. Liu SQ, Luo HS, Liu JL, McNeilly AS, Cui S. 2008. Specificity protein 1 (Sp1) plays role in regulating LIM homeodomain transcription factor Lhx4 gene expression. Biochem Biophys Res Commun 366:36-41.
31. Lugovskoy AA, Zhou P, Chou JJ, McCarty JS, Li P, Wagner G. 1999. Solution structure of the CIDE-N domain of CIDE-B and a model for CIDE-N/CIDE-N interactions in the DNA fragmentation pathway of apoptosis. Cell 99:747-755.
32. Ma NN, Ellet J, Okediadi C, Hermes P, McCormick E, Casnocha S. 2009. A single nutrient feed supports both chemically defined NS0 and CHO Fed-Batch processes: Improved productivity and lactate metabolism. Biotechnol Prog 25:1353-1363.
33. Marder P, Maciak RS, Fouts RL, Baker RS, Starling JJ. 1990. Selective cloning of hybridoma cells for enhanced immunoglobulin production using flow cytometric cell sorting and automated laser nephelometry. Cytometry 11:498-505.
34. Mauro C, Crescenzi E, De Mattia R, Pacifico F, Mellone S, Salzano S, de Luca C, D'Adamio L, Palumbo G, Formisano S, Vito P, Leonardi A. 2006. Central role of the scaffold protein tumor necrosis factor receptor-associated factor 2 in regulating endoplasmic reticulum stress-induced apoptosis. J Biol Chem 281:2631-2638.
35. McKenna SL, Cotter TG. 2000. Inhibition of caspase activity delays apoptosis in a transfected NS/0 myeloma cell line. Biotechnol Bioeng 67:165-176.
36. Ohya T, Hayashi T, Kiyama E, Nishii H, Miki H, Kobayashi K, Honda K, Omasa T, Ohtake H. 2008. Improved production of recombinant human antithrombin III in Chinese hamster ovary cells by ATF4 overexpression. Biotechnol Bioeng 100:317-324.
37. Prentice HL, Ehrenfels BN, Sisk WP. 2007. Improving performance of mammalian cells in fed-batch processes through "bioreactor evolution". Biotechnol Prog 23:458-464.
38. Rao RV, Hermel E, Castro-Obregon S, del Rio G, Ellerby LM, Ellerby HM, Bredesen DE. 2001. Coupling endoplasmic reticulum stress to the cell death program - Mechanism of caspase activation. J Biol Chem 276:33869-33874.
39. Samuels-Lev Y, O'Connor DJ, Bergamaschi D, Trigiante G, Hsieh JK, Zhong S, Campargue I, Naumovski L, Crook T, Lu X. 2001. ASPP proteins specifically stimulate the apoptotic function of p53. Mol Cell 8:781-794.
40. Sauerwald TM, Betenbaugh MJ, Oyler GA. 2002. Inhibiting apoptosis in mammalian cell culture using the caspase inhibitor XIAP and deletion mutants. Biotechnol Bioeng 77:704-716.
41. Schumpp B, Schlaeger EJ. 1992. Growth study of lactate and ammonia double-resistant clones of Hl-60 cells. Cytotechnology 8:39-44.
42. Shen D, Kiehl TR, Khattak SF, Li ZJ, He A, Kayne PS, Patel V, Neuhaus IM, Sharfstein ST. 2010. Transcriptomic responses to sodium chloride-induced osmotic stress: A study of industrial fed-batch CHO cultures. Biotechnol Prog (Epub ahead of print).
43. Simpson NH, Singh RP, Emery AN, Al-Rubeai M. 1999. Bcl-2 over-expression reduces growth rate and prolongs G(1) phase in continuous chemostat cultures of hybridoma cells. Biotechnol Bioeng 64:174-186.
44. Singh RP, Al-Rubeai M, Gregory CD, Emery AN. 1994. Cell death in bioreactors - a role for apoptosis. Biotechnol Bioeng 44:720-726.
45. Singh RP, Emery AN, AlRubeai M. 1996. Enhancement of survivability of mammalian cells by overexpression of the apoptosis-suppressor gene bcl-2. Biotechnol Bioeng 52:166-175.
46. Sung YH, Lee JS, Park SH, Koo J, Lee GM. 2007. Influence of co-down-regulation of caspase-3 and caspase-7 by siRNAs on sodium butyrate-induced apoptotic cell death of Chinese hamster ovary cells producing thrombopoietin. Metab Eng 9:452-464.
47. Sunstrom NAS, Gay RD, Wong DC, Kitchen NA, DeBoer L, Gray PP. 2000. Insulin-like growth factor-I and transferrin mediate growth and survival of Chinese hamster ovary cells. Biotechnol Prog 16:698-702.
48. Swiderek H, Al Rubeai M. 2007. Functional genome-wide analysis of antibody producing NS0 cell line cultivated at different temperatures. Biotechnol Bioeng 98:616-630.
49. Tey BT, Singh RP, Piredda L, Piacentini M, Al Rubeai M. 2000. Bcl-2 mediated suppression of apoptosis in myeloma NS0 cultures. J Biotechnol 79:147-159.
50. Tran NL, McDonough WS, Savitch BA, Sawyer TF, Winkles JA, Berens ME. 2005. The tumor necrosis factor-like weak inducer of apoptosis (TWEAK)-fibroblast growth factor-inducible 14 (Fn14) signaling system regulates glioma cell survival via NF kappa B pathway activation and BCL-XL/BCL-W expression. J Biol Chem 280:3483-3492.
51. Trummer E, Ernst W, Hesse F, Schriebl K, Lattenmayer C, Kunert R, Vorauer-Uhl K, Katinger H, Muller D. 2008. Transcriptional profiling of phenotypically different Epo-Fc expressing CHO clones by cross-species microarray analysis. Biotechnol J 3:924-927.
52. Walsh G, Jefferis R. 2006. Post-translational modifications in the context of therapeutic proteins. Nat Biotechnol 24:1241-1252.
53. Wong DCF, Wong KTK, Lee YY, Morin PN, Heng CK, Yap MGS. 2006. Transcriptional profiling of apoptotic pathways in batch and fed-batch CHO cell cultures. Biotechnol Bioeng 94:373-382.
54. Yoneda T, Imaizumi K, Oono K, Yui D, Gomi F, Katayama T, Tohyama M. 2001. Activation of caspase-12, an endoplastic reticulum (ER) resident caspase, through tumor necrosis factor receptor-associated factor 2-dependent mechanism in response to the ER stress. J Biol Chem 276:13935-13940.
55. Zanghi JA, Renner WA, Bailey JE, Fussenegger M. 2000. The growth factor inhibitor suramin reduces apoptosis and cell aggregation in protein-free CHO cell batch cultures. Biotechnol Prog 16:319-325.
56. Zhou WC, Chen CC, Buckland B, Aunins J. 1997. Fed-batch culture of recombinant NS0 myeloma cells with high monoclonal antibody production. Biotechnol Bioeng 55:783-792.
57. Zhou DH, Palam LR, Jiang L, Narasimhan J, Staschke KA, Wek RC. 2008. Phosphorylation of eIF2 directs ATF5 translational control in response to diverse stress conditions. J Biol Chem 283:7064-7073.
58. Zustiak MP, Pollack JK, Marten MR, Betenbaugh MJ. 2008. Feast or famine: Autophagy control and engineering in eukaryotic cell culture. Curr Opin Biotechnol 19:518-526.