The putative gymnosperm plant defensin polypeptide (SPI1) accumulates after seed germination, is not readily released, and the SPI1 levels are reduced in Pythium dimorphum-infected spruce roots

Plant Molecular Biology

Volumn 52, Issue 2, 2003, Pages 291-302

  Fossdal, Carl Gunnar ^a   Nagy, Nina Elisabeth ^a   Sharma, Praveen ^b   Lönneborg, Anders ^b  

^a NORWEGIAN INSTITUTE OF BIOECONOMY RESEARCH   (Norway)

^b Diagenic AS   (Norway)

Author keywords

Defence response; Picea abies; Plant defensin; Pythium dimorphum; Suppression

Indexed keywords

ANTIBODIES; CELLS; DNA; FUNGI; PLANTS (BOTANY); SEED; TISSUE;

CORTICAL ROOT CELLS;

POLYPEPTIDES;

DEFENSINS; FUNGI; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENE EXPRESSION REGULATION, PLANT; GERMINATION; IMMUNOBLOTTING; MICROSCOPY, CONFOCAL; PICEA; PLANT PROTEINS; PLANT ROOTS; PYTHIUM; RNA, MESSENGER; SEEDS; TIME FACTORS;

ABIES; CERATOCYSTIS POLONICA; CONIFEROPHYTA; FUNGI; GYMNOSPERMAE; HETEROBASIDION; HETEROBASIDION ANNOSUM; LACCARIA BICOLOR; PICEA; PICEA ABIES; PYTHIUM DIMORPHUM;

EID: 0038236366     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1023915230129     Document Type: Article

References (40)

1. Asiegbu, F.O., Daniel, G. and Johansson, M. 1994. Defense-related reactions of seedling roots of Norway spruce to infection by Heterobasidion annosum (Fr.) Bref. Physiol. Mol. Plant Path. 45: 1-19.
2. Asiegbu, F.O., Denekamp, M., Daniel, G. and Johansson, M. 1995. Immunocytochemical localization of pathogenesis-related proteins in roots of Norway spruce infected with Heterobasidion annosum. Eur. J. Forest Path. 25: 169-178.
3. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman J.G., Smith, J.A. and Struhl, K. eds. 1987. Current Protocols in Molecular Biology. John Wiley, New York.
4. Broekaert, W.F., Terras, F.R.G., Cammune, B.P.A. and Osborn, R.W. 1995. Plant defensins: novel antimicrobial peptides as components of the host defense system. Plant Physiol. 108: 1353-1358.
5. Børja, I., Sharma, P., Krekling, T. and Lönneborg, A. 1995. Cytopathological response in roots of Picea abies seedlings infected with Pythium dimorphum. Phytopathology 85: 495-501.
6. Christiansen, E. and Solheim, H. 1990. The bark beetle-associated blue-stain fungus Ophiostoma polonicum can kill various spruces and Douglas fir. Eur. J. Forest Path. 20: 436-446.
7. Dixon, R.A. and Harrison, M.J. 1990. Activation, structure, and organization of genes involved in microbial defense in plants. Adv. Genet. 28: 165-234.
8. Elfstrand, M., Fossdal, C.G., Swedjemark, G., Clapham, D., Olsson, O., Sitbon, F., Sharma, P., Lönneborg, A. and von Arnold, S. 2001. Identification of candidate genes for use in molecular breeding: a case study with the Norway spruce defensin-like gene, SP11. Silvae Genet. 50: 75-81.
9. Eltrop, L. and Marschner, H. 1996. Growth and mineral nutrition of non-mycorrhizal and mycorrhizal Norway spruce (Picea abies) seedlings grown in semi-hydroponic sand culture. I. Growth and mineral nutrient uptake in plants supplied with different forms of nitrogen. New Phytol. 133: 469-478.
10. Ekramoddoullah, A.K and Davidson, J.J. 1995. A method for the determination of conifer foliage protein extracted using sodium dodecyl sulphate and mercaptoethanol. Phytochem. Anal. 6: 20-24.
11. Epple, P., Apel, K., and Bohlmann, H., 1997. ESTs reveal a multigene family for plant defensins in Arabidopsis thaliana. FEBS Lett. 400: 168-172.
12. Fossdal, C.G. 1999. Molecular and cytological responses in root-infected Norway spruce [Picea abies (L.) Karst.] seedlings. Ph.D. Dissertation, Agricultural University of Norway, Ås, Norway.
13. Fossdal, C.G., Sharma, P. and Lönneborg, A. 2001. Isolation of the first putative plant peroxidase cDNA from a conifer and the local and systemic accumulation of related proteins upon pathogen infection. Plant Mol. Biol. 47: 423-435.
14. Gu, Q., Kamata, E.E., Morse, M.J., Wu, H.M. and Cheung, A.Y. 1992. A flower-specific cDNA encoding a novel thionin in tobacco. Mol. Gen. Genet. 234: 89-96.
15. Karabaghli-Degron, C., Sotta, B., Bonnet, M., Gay, G. and LeTacon, F. 1998. The auxin transport inhibitor 2,3,5-triiodobenzoic acid (TIBA) inhibits the stimulation of in vitro lateral root formation and the colonization of the tap-root cortex of Norway spruce (Picea abies) seedlings by the ectomycorrhizal fungus Laccaria bicolor. New Phytol. 140: 723-733.
16. Kozlowski, G. and Metraux, J.P. 1998. Infection of Norway spruce (Picea abies (L.) Karst.) seedlings with Pythium irregulare Buism. and Pythium ultimum Trow: histological and biochemical responses. Eur. J. Plant Path. 104: 225-234.
17. Kozlowski, G., Buchala, A. and Metraux, J.P. 1999. Methyl jasmonate protects Norway spruce (Picea abies (L.) Karst.) seedlings against Pythium ultimum Trow. Physiol. Mol. Plant Path. 55: 53-58.
18. Kristensen, A.K., Brunstedt, J., Nielsen, J.E., Mikkelsen, J.D., Roepstorff, P. and Nielsen, K.K. 1999. Processing, disulfide pattern, and biological activity of a sugar beet defensin, AX2, expressed in Pichia pastoris. Protein Expr. Purif. 16: 377-387.
19. Lönneborg, A. and Jensen, M. 2000. Reliable and reproducible method to extract high-quality RNA from plant tissues rich in secondary metabolites. Biotechniques 29: 714-718.
20. Manners, J.M., Penninckx, I.A., Vermaere, K., Kazan, K., Brown, R.L., Morgan, A., Maclean, D.J., Curtis, M.D., Cammue, B.P. and Broekaert, W.F. 1998. The promoter of the plant defensin gene PDF1.2 from Arabidopsis is systemically activated by fungal pathogens and responds to methyl jasmonate but not to salicylic acid. Plant. Mol. Biol. 8: 1071-1080.
21. Menedez, E., Moreno, A., Collila, F., Pelaez, F., Limas, G.G., Mendez, R., Soriano, F., Salinas, M. and DeHaro, C. 1990. Primary structure and inhibition of protein synthesis in eukaryotic cell-free system of a novel thionin, γ-hordothionin, from barley endosperm. Eur. J. Biochem. 194: 533.
22. Moreno, M., Segura, A. and Garcia-Olmedo, F. 1994. Pseudothionin-ST1, a potato peptide active against potato pathogens. Eur. J. Biochem. 223: 135-139.
23. Nagy, N.E., Franceschi, V.R., Solheim, H., Krekling, T. and Christiansen, E. 2000. Wound-induced traumatic resin duct formation in stems of Norway spruce (Pinaceae): anatomy and cytochemical traits. Am. J. Bot. 87: 302-313.
24. Osborn, R.W., Desamblanx, G.W., Thevissen, K., Goderis, I., Torrekenes, S., vanLeuven, F., Attenborough, S., Rees, S.B. and Broekaert, W.F. 1995. Isolation and characterization of plant defensins from seeds of Asteracea, Fabaceae, Hippocastanaceae and Saxifragaceae. FEBS Lett. 368: 257-262.
25. Pappinen, A., and von Weissenberg, K. 1997. Detection and partial characterization of extracellular proteases of the pathogenic fungi Endocronartium pini, Gremmeniella abietina and Heterobasidion annosum. Eur. J. Plant Path. 27: 373-380.
26. Schagger, H. and von Jagow, G. 1987. Tricine sodium dodecylsulfate polyacrylamide-gel electrophoresis for the separation of proteins in the range from 1kD to 100kD. Anal. Biochem. 166: 368-379.
27. Segura, A., Moreno, M., Molina, A. and Garcia-Olmedo, F. 1998. Novel defensin subfamily from spinach (Spinacia oleracea). FEBS Lett. 435: 159-162.
28. Sharma, P. 1995. Induced defence responses in spruce (Picea abies (L.) Karst.) roots during infection with a pathogenic fungus, Pythium dimorphum. Ph.D. Dissertation, Agricultural University of Norway, Ås, Norway.
29. Sharma, P. and Lönneborg, A. 1996. Isolation and characterization of a cDNA encoding a plant defensin-like protein from roots of Norway spruce. Plant Mol. Biol. 3: 707-312.
30. Tarlton, J.F. and Knight, P.J. 1996. Comparison of reflectance and transmission densitometry, using document and laser scanners, for quantitation of stained western blots. Anal. Biochem. 237: 123-128.
31. Terras, F.R., Schoofs, H.M., DeBolle, M.F., vanLeuven, F., Rees, S.B., Vanderleyden, J., Cammue, B.P. and Broekaert, W.F. 1992. Analysis of 2 novel classes of plant antifungal proteins from radish seeds. J. Biol. Chem. 267: 15301-15309.
32. Terras, F.R., Torrekenes, S., vanLeuven, F., Osborn, R.W., Vanderleyden, J., Cammue, B.P. and Broekaert, W.F. 1993. A new family of basic cysteine-rich plant antifungal proteins from Brassicaceae species. FEBS Lett. 316: 233-240.
33. Terras, F.R., Eggermont, K., Kovaleva, V., Raikhel, N.V., Osborn, R.W., Kester, A., Rees, S.B., Torrekenes, S., vanLeuven, F., Vanderleyden, J., Cammue, B.P. and Broekaert, W.F. 1995. Small cysteine-rich antifungal proteins from radish: their role in host defense. Cell 7: 573-588.
34. Thevissen, K., Ghazi, A., DeSamblanx, G.W., Brownlee, C., Osborn, R.W. and Broekaert, W.F. 1996. Fungal membrane responses induced by plant defensins and thionins. J. Biol. Chem. 271: 15018-15025.
35. Thevissen, K., Osborne, R.W., Acland, D.P. and Broekaert, W.F. 1997. Specific, high affinity binding sites for an antifungal plant defensin on Neurospora crassa hyphae and microsomal membranes. J. Biol. Chem. 272: 32176-32181.
36. Thevissen, K., Osborn, R.W., Acland, D.P. and Broekaert, W.F. 2000. Specific binding sites for an antifungal plant defensin from dahlia (Dahlia merckii) on fungal cells are required for antifungal activity. Mol. Plant.-Microbe Interact. 13: 54-61.
37. Thomma, B.P. and Broekaert, W.F. 1998. Tissue-specific expression of plant defensin genes PDF2.1 and PDF2.2 in Arabidopsis thaliana. Plant Physiol. Biochem. 36: 533-537.
38. Vad, K., Mikkelsen J.D., and Collinge D.B., 1991. Induction, purification and characterization of chitinase isolated from pea leaves inoculated with Ascochyta pisi. Planta 184: 24-29.
39. van Loon, L.C. 1985. Pathogenesis related proteins. Plant Mol. Biol. 4: 111-116.
40. van Loon, L.C. 1998. The 5th Int. workshop on pathogenesis-related proteins in plant signaling and biological activities. In: Woodward, S., Stenlid, J., Karjalainen, R. and Hüttermann, A. (eds.). 1998. Heterobasidion annosum: Biology, Ecology, Impact and Control. CAB International, Oxford, UK, PR98 abstract L1.