Differential antifungal and calcium channel-blocking activity among structurally related plant defensins

Plant Physiology

Volumn 135, Issue 4, 2004, Pages 2055-2067

  Spelbrink, Robert G ^a   Dilmac, Nejmi ^b   Allen, Aron ^a   Smith, Thomas J ^a   Shah, Dilip M ^a   Hockerman, Gregory H ^b  

^a DONALD DANFORTH PLANT SCIENCE CENTER   (United States)

^b PURDUE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGI; PROTEINS;

ANTIFUNGAL ACTIVITIES; PLANT DEFENSINS;

PLANTS (BOTANY);

FUNGI; PLANTS; PROTEINS;

FUNGI; FUSARIUM; FUSARIUM GRAMINEARUM; GIBBERELLA ZEAE; MAMMALIA; MEDICAGO; MEDICAGO SATIVA; MEDICAGO TRUNCATULA; RAPHANUS SATIVUS; USTILAGO; USTILAGO MAYDIS;

EID: 4444295137     PISSN: 00320889     EISSN: None     Source Type: Journal    
DOI: 10.1104/pp.104.040873     Document Type: Article

References (64)

1. Almeida MS, Cabral KM, Kurtenbach E, Almeida FC, Valente AP (2002) Solution structure of Pisum sativum defensin 1 by high resolution NMR: plant defensins, identical backbone with different mechanisms of action. J Mol Biol 315: 749-757
2. Bloch C, Richardson M (1991) A new family of small (5 kDa) protein inhibitors of insect alpha-amylases from seeds of Sorghum (Sorghum bicolor (L) Moench) have sequence homologies with wheat gammapurothionins. FEBS Lett 279: 101-105
3. Bontems F, Roumestand C, Boyot P, Gilquin B, Doljansky Y, Menez A, Toma F (1991) Three-dimensional structure of natural charybdotoxin in aqueous solution by 1H-NMR. Charybdotoxin possesses a structural motif found in other scorpion toxins. Eur J Biochem 196: 19-28
4. Borkovich KA, Alex LA, Yarden O, Freitag M, Turner GE, Read ND, Seller S, Bell-Pedersen D, Paietta J, Plesofsky N, et al (2004) Lessons from the genome sequence of Neurospora crassa: tracing the path from genomic blueprint to multicellular organism. Microbiol Mol Biol Rev 68: 1-108 (table of contents)
5. Broekaert WF, Terras FR, Cammue BP, Osborn RW (1995) Plant defensins: novel antimicrobial peptides as components of the host defense system. Plant Physiol 108: 1353-1358
6. Broekaert WF, Terras FR, Cammue BP, Vanderleyden J (1990) An automated quantitative assay for fungal growth inhibition. FEMS Microbiol Lett 69: 55-60
7. 3+: a mechanistic study. J Gen Microbiol 138: 1791-1795
8. 2+ transport in Saccharomyces cerevisiae. J Exp Biol 196: 157-166
9. Davis TN (1995) Calcium in Saccharomyces cerevisiae. Adv Second Messenger Phosphoprotein Res 30: 339-358
10. De Samblanx GW, Goderis IJ, Thevissen K, Raemaekers R, Fant F, Borremans F, Acland DP, Osborn RW, Patel S, Broekaert WF (1997) Mutational analysis of a plant defensin from radish (Raphanus sativus L.) reveals two adjacent sites important for antifungal activity. J Biol Chem 272: 1171-1179
11. De Weille JR, Schweitz H, Maes P, Tartar A, Lazdunski M (1991) Calciseptin, a peptide isolated from black mamba venom, is a specific blocker of the L-type calcium channel. Proc Natl Acad Sci USA 88: 2437-2440
12. Durrenberger F, Wong K, Kronstad JW (1998) Identification of a cAMP-dependent protein kinase catalytic subunit required for virulence and morphogenesis in Ustilago maydis. Proc Natl Acad Sci USA 95: 5684-5689
13. Ellis SB, Williams ME, Ways NR, Brenner R, Sharp AH, Leung AT, Campbell KP, McKenna E, Koch WJ, Hui A, et al (1988) Sequence and expression of mRNAs encoding the a1 and a2 subunits of a DHP-sensitive calcium channel. Science 241: 1661-1664
14. Fant F, Vranken W, Broekaert W, Borremans F (1998) Determination of the three-dimensional solution structure of Raphanus sativus antifungal protein 1 by 1H NMR. J Mol Biol 279: 257-270
15. Ferket KK, Levery SB, Park C, Cammue BP, Thevissen K (2003) Isolation and characterization of Neurospora crassa mutants resistant to antifungal plant defensins. Fungal Genet Biol 40: 176-185
16. Fischer M, Schnell N, Chattaway J, Davies P, Dixon G, Sanders D (1997) The Saccharomyces cerevisiae CCH1 gene is involved in calcium influx and mating. FEBS Lett 419: 259-262
17. Fontecilla-Camps J-C (1989) Three-dimensional model of the insect-directed scorpion toxin from Androctonus australis Hector and its implication for the evolution of scorpion toxins in general. J Mol Evol 29: 63-67
18. Gage MJ, Bruenn J, Fischer M, Sanders D, Smith TJ (2001) KP4 fungal toxin inhibits growth in Ustilago maydis by blocking calcium uptake. Mol Microbiol 41: 775-786
19. Gage MJ, Rane SG, Hockerman GH, Smith TJ (2002) The virally encoded fungal toxin KP4 specifically blocks L-type voltage-gated calcium channels. Mol Pharmacol 61: 936-944
20. Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, et al (2003) The genome sequence of the filamentous fungus Neurospora crassa. Nature 422: 859-868
21. Gao A, Hakimi SM, Mittanck CA, Wu Y, Woerner MB, Stark DM, Shah DM, Liang J, Rommens CMT (2000) Fungal pathogen protection in potato by expression of a plant defensin peptide. Nat Biotechnol 18: 1307-1310
22. Garcia ML, Gao Y, McManus OB, Kaczorowski GJ (2001) Potassium channels: from scorpion venoms to high-resolution structure. Toxicon 39: 739-748
23. Gold S, Duncan G, Barrett K, Kronstad J (1994) cAMP regulates morphogenesis in the fungal pathogen Ustilago maydis. Genes Dev 8: 2805-2816
24. 2+ channel inhibitor. Structure 3: 805-814
25. Gu F, Sullivan TS, Che Z, Ganesa C, Flurkey WH, Bozarth RF, Smith TJ (1994) The characterization and crystallization of a virally encoded Ustilago maydis KP4 toxin. J Mol Biol 243: 792-795
26. Housset D, Habersetzer-Rochat C, Astier J-P, Fontecilla-Camps JC (1994) Crystal structure of toxin II from scorpion Androctonus australis Hector refined at 1.3Å resolution. J Mol Biol 238: 88-104
27. 2+ in Saccharomyces cerevisiae. J Biol Chem 265: 21216-21222
28. Jackson SL, Heath IB (1993) Roles of calcium ion in hyphal tip growth. Microbiol Rev 57: 367-382
29. Kanzaki M, Nagasawa M, Kojima I, Sato C, Naruse K, Sokabe M, Iida H (1999) Molecular identification of a eukaryotic, stretch-activated non-selective cation channel. Science 285: 882-886
30. Kobayashi Y, Takashima H, Tamaoki H, Kyogoku Y, Lambert P, Kuroda H, Chino N, Wantanabe TX, Kimura T, Sakakibara S (1991) The cysteine-stabilized alpha-helix: a common structural motif of ion-channel blocking neurotoxic peptide. Biopolymers 31: 1213-1220
31. Kushmerick C, de Souza Castro M, Santos Cruz J, Bloch C Jr, Beirao PS (1998) Functional and structural features of gamma-zeathionins, a new class of sodium channel blockers. FEBS Lett 440: 302-306
32. Lay FT, Schirra HJ, Scanlon MJ, Anderson MA, Craik DJ (2003) The three-dimensional solution structure of NaD1, a new floral defensin from Nicotiana alata and its application to a homology model of crop defense protein alfAFP. J Mol Biol 325: 175-188
33. 2+ and plasma membrane ion channels in hyphal tip growth of Neurospora crassa. J Cell Sci 108: 3405-3417
34. Liang J, Shah DM, Wu Y, Rosenberger CA, Hakimi SM, inventors (2001) Antifungal polypeptide from alfalfa and methods for controlling plant pathogenic fungi. US Patent No. 6,316,407
35. MacKinnon R, Miller C (1989) Mutant potassium channels with altered binding of charybdotoxin, a pore-blocking peptide inhibitor. Science 245: 1382-1385
36. Muller EM, Locke EG, Cunningham KW (2001) Differential regulation of two Ca(2+) influx systems by pheromone signaling in Saccharomyces cerevisiae. Genetics 159: 1527-1538
37. Nelson G, Kozlova-Zwinderman O, Collis AJ, Knight MR, Fincham JRS, Stanger CP, Renwick A, Hessing JGM, Punt PJ, van den Hondel CAMJJ, et al (2004) Calcium measurement in living filamentous fungi expressing codon-optimised aequorin. Mol. Microbiol. 52: 1437-1450
38. 2+ uptake and exacerbates the cdc1(Ts) growth defect. Mol Cell Biol 17: 6339-6347
39. Palmer CP, Zhou XL, Lin J, Loukin SH, Kung C, Saimi Y (2001) A TRP homolog in Saccharomyces cerevisiae forms an intracellular Ca(2+)-permeable channel in the yeast vacuolar membrane. Proc Natl Acad Sci USA 98: 7801-7805
40. Park CM, Bruenn JA, Ganesa C, Flurkey WF, Bozarth RF, Koltin Y (1994) Structure and heterologous expression of Ustilago maydis viral toxin KP4. Mol Microbiol 11: 155-164
41. Pragnell M, Sakamoto J, Jay SD, Campbell KP (1991) Cloning and tissue-specific expression of the brain calcium channel beta-subunit. FEBS Lett 291: 253-258
42. Segura A, Moreno M, Molina A, Garcia-Olmedo F (1998) Novel defensin subfamily from spinach (Spinacia oleracea). FEBS Lett 435: 159-162
43. 2+] gradient in growing hyphae of the fungus Neurospora crassa. Eur J Cell Biol 80: 379-390
44. 2+ channel regulates fungal tip growth. J Cell Sci 115: 5013-5025
45. Snutch TP, Tomlinson WJ, Leonard JP, Gilbert MM (1991) Distinct calcium channels are generated by alternative splicing and are differentially expressed in the mammalian CNS. Neuron 7: 45-57
46. Soong T, Stea A, Hodson CD, Dubel SJ, Vincent SR, Snutch TP (1993) Structure and functional expression of a member of the low voltage-activated calcium channel family. Science 260: 1133-1136
47. Teramoto N, Ogata R, Okabe K, Kameyama A, Kameyama M, Watanabe TX, Kuriyama H, Kitamura K (1996) Effects of calciseptine on unitary barium channel currents in guinea-pig portal vein. Pflugers Arch 432: 462-470
48. Terlau H, Heinemann SH, Stuhmer W, Pusch M, Conti F, Imoto K, Numa S (1991) Mapping the site of block by tetrodotoxin and saxitoxin of sodium channel II. FEBS Lett 293: 93-96
49. Terras FR, Eggermont K, Kovaleva V, Raikhel NV, Osborn RW, Kester A, Rees SB, Torrekens S, Van Leuven F, Vanderleyden J, et al (1995) Small cysteine-rich antifungal proteins from radish: their role in host defense. Plant Cell 7: 573-588
50. Terras FR, Schoofs HM, De Bolle MF, Van Leuven F, Rees SB, Vanderleyden J, Cammue BP, Broekaert WF (1992) Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds. J Biol Chem 267: 15301-15309
51. Terras FR, Torrekens S, Van Leuven F, Osborn RW, Vanderleyden J, Cammue BP, Broekaert WF (1993) A new family of basic cysteine-rich plant antifungal proteins from Brassicaceae species. FEBS Lett 316: 233-240
52. Thevissen K, Cammue BP, Lemaire K, Winderickx J, Dickson RC, Lester RL, Ferket KK, Van Even F, Parret AH, Broekaert WF (2000) A gene encoding a sphingolipid biosynthesis enzyme determines the sensitivity of Saccharomyces cerevisiae to an antifungal plant defensin from dahlia (Dahlia merckii). Proc Natl Acad Sci USA 97: 9531-9536
53. Thevissen K, Ghazi A, De Samblanx GW, Brownlee C, Osborn RW, Broekaert WF (1996) Fungal membrane responses induced by plant defensins and thionins. J Biol Chem 271: 15018-15025
54. Thevissen K, Osborn RW, Acland DP, Broekaert WF (1997) Specific, high affinity binding sites for an antifungal plant defensin on Neurospora crassa hyphae and microsomal membranes. J Biol Chem 272: 32176-32181
55. Thevissen K, Osborn RW, Acland DP, Broekaert WF (2000) Specific binding sites for an antifungal plant defensin from dahlia (Dahlia merckii) on fungal cells are required for antifungal activity. Mol Plant Microbe Interact 13: 54-61
56. Thevissen K, Terras FR, Broekaert WF (1999) Permeabilization of fungal membranes by plant defensins inhibits fungal growth. Appl Environ Microbiol 65: 5451-5458
57. Thevissen K, Warnecke DC, Francois IE, Leipelt M, Heinz E, Ott C, Zahringer U, Thomma BP, Ferket KK, Cammue BP (2004) Defensins from insects and plants interact with fungal glucosylceramides. J Biol Chem 279: 3900-3905
58. Thomma BP, Cammue BP, Thevissen K (2003) Mode of action of plant defensins suggests therapeutic potential. Curr Drug Targets Infect Disord 3: 1-8
59. Thomma BPHJ, Camme BPA, Thevissen K (2002) Plant defensins. Planta 216: 193-202
60. 2+-channel diversity, 1988-1994. Trends Neurosci 18: 52-54
61. Tsien RW, Tsien RY (1990) Calcium channels, stores and isolations. Annu Rev Cell Biol 6: 715-760
62. Vogel HJ (1964) Distribution of lysine pathways among fungi: evolutionary implications. Am Nat 98: 435-446
63. Wijaya R, Neumann GM, Condron R, Hughes AB, Polya GM (2000) Defense proteins from seed of Cassia fistula include a lipid transfer protein homologue and a protease inhibitory plant defensin. Plant Sci 159: 243-255
64. Young T (1987) Killer yeasts. In AH Rose, JS Harrison, eds, The Yeasts, Vol 2. Academic Press, Orlando, FL, pp 131-164