Purification and molecular cloning of antimicrobial peptides from Scots pine seedlings

Peptides

Volumn 30, Issue 12, 2009, Pages 2136-2143

  Kovaleva, Valentina ^a   Kiyamova, Ramziya ^b   Cramer, Rainer ^c   Krynytskyy, Hryhoriy ^a   Gout, Ivan ^b,d   Filonenko, Valeriy ^b   Gout, Roman ^a  

^a UKRAINIAN NATIONAL FORESTRY UNIVERSITY   (Ukraine)

^b INSTITUTE OF MOLECULAR BIOLOGY AND GENETICS   (Ukraine)

^c UNIVERSITY OF READING   (United Kingdom)

^d UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Antifungal activity; Antimicrobial peptides; Defensins; Molecular cloning; Purification; Scots pine

Indexed keywords

AMINO ACID; AMMONIUM SULFATE; CELLULOSE PHOSPHATE; COMPLEMENTARY DNA; DEFENSIN; LIPID TRANSFER PROTEIN; POLYPEPTIDE ANTIBIOTIC AGENT; SULFURIC ACID;

ANTIBACTERIAL ACTIVITY; ANTIFUNGAL ACTIVITY; ANTIMICROBIAL ACTIVITY; ARTICLE; CONTROLLED STUDY; DNA ISOLATION; DNA LIBRARY; ENDOPLASMIC RETICULUM; ERWINIA AMYLOVORA; ESCHERICHIA COLI; EXTRACTION; FUNGUS; FUNGUS CULTURE; ION EXCHANGE CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PINE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PURIFICATION; SEEDLING;

AMINO ACID SEQUENCE; ANTI-INFECTIVE AGENTS; BASE SEQUENCE; CLONING, MOLECULAR; DEFENSINS; GENE EXPRESSION REGULATION, PLANT; MOLECULAR SEQUENCE DATA; PEPTIDES; PHYLOGENY; PINUS SYLVESTRIS; PLANT PROTEINS; SEEDLING; SEQUENCE ALIGNMENT;

FUNGI; PINUS SYLVESTRIS;

EID: 70449672643     PISSN: 01969781     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.peptides.2009.08.007     Document Type: Article

References (31)

1. Almeida M.S., Cabral K.M., Kurtenbach E., Almeida F.C., and Valente A.P. Solution structure of Pisum sativum defensin 1 by high resolution NMR: plant defensins, identical backbone with different mechanisms of action. J Mol Biol 315 (2002) 749-757
2. Broekaert W., Cammue B., DeBolle M., Thevissen K., DeSamblanx G., and Osborn R. Antimicrobial peptides from plants. Crit Rev Plant Sci 16 (1997) 297-323
3. Carvalho A.O., Filho G.A., Ferreira B.S., Branco A.T., Okorokova-Façanha A.L., and Gomes V.M. Cloning and characterization of a cDNA encoding a cowpea seed defensin and analysis of its expression. Protein Pept Lett 13 10 (2006) 1029-1036
4. Carvalho A.O., and Gomes V.M. Role of plant lipid transfer proteins in plant cell physiology-a concise review. Peptides 28 5 (2007) 1144-1153
5. Carvalho A.O., Souza-Filho G.A., Ferreira B.S., Branco A.T., Araújo I.S., Fernandes K.V.S., et al. Cloning and characterization of a cowpea seed lipid transfer protein cDNA: expression analysis during seed development and under fungal and cold stresses in seedlings' tissues. Plant Physiol Biochem 44 (2006) 732-742
6. Castro M.S., and Fontes W. Plant defense and antimicrobial peptides. Protein Pept Lett 12 (2005) 13-18
7. Chang S., Puryear J., and Cairney J. A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11 (1993) 113-116
8. De Bolle M.F., Goderis I.J., Terras F.R., Cammue B.P., and Broekaert W.F. A technique for detecting antifungal activity of proteins separated by polyacrylamide gel electrophoresis. Electrophoresis 12 6 (1991) 442-444
9. De Samblanx G.W., Goderis I., Thevissen K., Raemaekers R., Fant F., Borremans F., et al. Mutational analysis of plant defensin from radish (Raphanus sativus L.) reveals two adjacent sites important for antifungal activity. J Biol Chem 272 (1997) 1171-1179
10. Do H.M., Lee S.C., Jung H.W., Sohn K.H., and Hwang B.K. Differential expression and in situ localization of a pepper defensin (CADEF1) gene in response to pathogen infection, abiotic elicitors and environmental stresses in Capsicum annuum. Plant Sci 166 (2004) 1297-1305
11. Epple P., Apel K., and Bohlmann H. ESTs reveal a multigene family for plant defensins in Arabidopsis thaliana. FEBS Lett 400 (1997) 168-172
12. Fossdal C., Nagy N., Sharma P., and Lonneborg A. The putative gymnosperm plant defensin polypeptide (SPI1) accumulates after seed germination, is not readily released, and the SPI1 levels are reduced in Pythium dimorphum-infected spruce roots. J Plant Mol Biol 52 (2003) 291-302
13. Jenssen H., Hamill P., and Hancock R.E.W. Peptide antimicrobial agents. Clin Microbiol Rev 19 (2006) 491-511
14. Koike M., Okamoto T., Tsuda S., and Imai R. A novel plant defensin-like gene of winter wheat is specifically induced during cold acclimation. Biochem Biophys Res Commun 298 (2002) 46-53
15. Kovalyova V., and Gout R. Isolation of the proteins with antifungal activity from Pinus sylvestris seedlings. Physiol Biochem Cultiv Plants 39 (2007) 114-120
16. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 5259 (1970) 680-685
17. Lay F.T., Brugliera F., and Anderson M.A. Isolation and properties of floral defensins from ornamental tobacco and petunia. Plant Physiol 131 (2003) 1283-1293
18. Manners J. Hidden weapons of microbial destruction in plant genomes. Genome Biol 8 (2007) 225
19. Mirouze M., Sels J., Richard O., Czernic P., Loubet S., Jacquier A., et al. A putative novel role for plant defensins: a defensin from the zinc hyper-accumulating plant, Arabidopsis halleri, confers zinc tolerance. Plant J 47 (2006) 329-342
20. Nagano K., Masters J.R., Akpan A., Yang A., Corless S., Wood C., et al. Differential protein synthesis and expression levels in normal and neoplastic human prostate cells and their regulation by type I and II interferons. Oncogene 23 (2004) 1693-1703
21. Pagnussat L.A., Lombardo C., Regente M., Pinedo M., Martín M., and de la Canal L.J. Unexpected localization of a lipid transfer protein in germinating sunflower seeds. Plant Physiol 166 8 (2009) 797-806
22. Pervieux I., Bourassa M., Laurans F., Hamelin R., and Seguin A. A spruce defensin showing strong antifungal activity and increased transcript accumulation after wounding and jasmonate treatments. Physiol Mol Plant Pathol 64 (2004) 331-341
23. Schägger H., and Von Jagow G. Tricine-sodium dodecylsulfate polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166 (1987) 368-379
24. Sharma P., and Lonneborg A. Isolation and characterization of a cDNA encoding a plant defensin-like protein from roots of Norway spruce. J Plant Mol Biol 31 (1996) 707-712
25. Shevchenko A., Wilm M., Vorm O., and Mann M. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem 68 5 (1996) 850-858
26. Silvestein K., Graham M., Paape T., and VandenBosh K. Genome organization of more 300 defensin-like genes in Arabidopsis. Plant Physiol 138 (2005) 600-610
27. Terras F.R., Eggermont K., Kovaleva V., Raikhel N.V., Osborn R.W., Kester A., et al. Small cysteine-rich antifungal proteins from radish: their role in host defense. Plant Cell 7 (1995) 573-588
28. Terras F.R.G., Schoofs H.M.E., De Bolle M.F.C., Van Leuven F., Rees S.B., Vanderleyden J., et al. Analysis of two novel classes of antifungal proteins from radish (Raphanus sativus L.) seeds. J Biol Chem 267 (1992) 5301-5309
29. Thevissen K., Osborn R., Acland D., and Broekaert W. Specific binding sites for an antifungal plant defensin from dahlia (Dahlia merckii) on fungal cells are required for antifungal activity. Mol Plant Microbe Interact 31 (2000) 54-61
30. Thomma B.P., Cammue B.P., and Thevissen K. Plant defensins. Planta 216 (2002) 193-202
31. Yeats T.H., and Rose J.K. The biochemistry and biology of extracellular plant lipid-transfer proteins (LTPs). Protein Sci 17 (2008) 191-198