Functional analysis of bispecific antibody (EpCAMxCD3)-mediated T-lymphocyte and cancer cell interaction by single-cell force spectroscopy

International Journal of Cancer

Volumn 128, Issue 9, 2011, Pages 2096-2104

  Hoffmann, Sabrina C ^a,b   Wabnitz, Guido H ^a   Samstag, Yvonne ^b   Moldenhauer, Gerhard ^b   Ludwig, Thomas ^b  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

Atomic force microscopy; Bispecific antibody; Single cell force spectroscopy; Synapse formation; T cell immunology

Indexed keywords

BISPECIFIC ANTIBODY; EPCAM ANTIBODY; OKT 3; UNCLASSIFIED DRUG;

ANTIBODY SPECIFICITY; ARTICLE; ATOMIC FORCE MICROSCOPY; CANCER CELL; CELL ADHESION; CELL CONTACT; CELL INTERACTION; CELLULAR IMMUNITY; CONTROLLED STUDY; FUNCTIONAL ASSESSMENT; HUMAN; HUMAN CELL; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; SINGLE CELL FORCE SPECTROSCOPY; SPECTROSCOPY; T LYMPHOCYTE; T LYMPHOCYTE ACTIVATION;

ANTIBODIES, BISPECIFIC; ANTIGENS, CD3; ANTIGENS, NEOPLASM; CELL ADHESION; CELL ADHESION MOLECULES; CELL COMMUNICATION; CELL LINE, TUMOR; CELL SEPARATION; FLOW CYTOMETRY; HUMANS; IMMUNOLOGICAL SYNAPSES; LYMPHOCYTE ACTIVATION; MICROSCOPY, ATOMIC FORCE; NEOPLASMS; SPECTRUM ANALYSIS; T-LYMPHOCYTES;

EID: 79952468378     PISSN: 00207136     EISSN: 10970215     Source Type: Journal    
DOI: 10.1002/ijc.25556     Document Type: Article

References (27)

1. Rabinovich GA, Gabrilovich D, Sotomayor EM. Immunosuppressive strategies that are mediated by tumor cells. Annu Rev Immunol 2007;25:267-96. (Pubitemid 46697910)
2. Fischer N, Leger O. Bispecific antibodies: molecules that enable novel therapeutic strategies. Pathobiology 2007;74:3-14.
3. Lum LG, Davol PA, Lee RJ. The new face of bispecific antibodies: targeting cancer and much more. Exp Hematol 2006;34:1-6. (Pubitemid 43069594)
4. Strauss G, Guckel B, Wallwiener D, Moldenhauer G. Without prior stimulation, tumor-associated lymphocytes from malignant effusions lyse autologous tumor cells in the presence of bispecific antibody HEA125xOKT3. Clin Cancer Res 1999;5:171-80. (Pubitemid 29045191)
5. Momburg F, Moldenhauer G, Hammerling GJ, Moller P. Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues. Cancer Res 1987;47:2883-91. (Pubitemid 17113431)
6. Went PT, Lugli A, Meier S, Bundi M, Mirlacher M, Sauter G, Dirnhofer S. Frequent EpCam protein expression in human carcinomas. Hum Pathol 2004;35:122-8. (Pubitemid 38133932)
7. Al-Hajj M, Wicha MS, Benito-Hernandez A, Morrison SJ, Clarke MF. Prospective identification of tumorigenic breast cancer cells. Proc Natl Acad Sci USA 2003;100:3983-8. (Pubitemid 36418143)
8. Dalerba P, Dylla SJ, Park IK, Liu R, Wang X, Cho RW, Hoey T, Gurney A, Huang EH, Simeone DM, Shelton AA, Parmiani G, et al. Phenotypic characterization of human colorectal cancer stem cells. Proc Natl Acad Sci USA 2007;104:10158-63. (Pubitemid 47175279)
9. Li C, Heidt DG, Dalerba P, Burant CF, Zhang L, Adsay V, Wicha M, Clarke MF, Simeone DM. Identification of pancreatic cancer stem cells. Cancer Res 2007;67:1030-7.
10. Marme A, Strauss G, Bastert G, Grischke EM, Moldenhauer G. Intraperitoneal bispecific antibody (HEA125xOKT3) therapy inhibits malignant ascites production in advanced ovarian carcinoma. Int J Cancer 2002;101:183-9. (Pubitemid 34970911)
11. Franz CM, Taubenberger A, Puech PH, Muller DJ. Studying integrin-mediated cell adhesion at the single-molecule level using AFM force spectroscopy. Sci STKE 2007:pl5.
12. Ludwig T, Kirmse R, Poole K, Schwarz US. Probing cellular microenvironments and tissue remodeling by atomic force microscopy. Pflugers Arch 2008;456:29-49.
13. Benoit M, Gabriel D, Gerisch G, Gaub HE. Discrete interactions in cell adhesion measured by single-molecule force spectroscopy. Nat Cell Biol 2000;2:313-7.
14. Binnig G, Quate CF, Gerber C. Atomic force microscope. Phys Rev Lett 1986;56:930-3.
15. Helenius J, Heisenberg CP, Gaub HE, Muller DJ. Single-cell force spectroscopy. J Cell Sci 2008;121:1785-91. (Pubitemid 351943371)
16. Taubenberger A, Cisneros DA, Friedrichs J, Puech PH, Muller DJ, Franz CM. Revealing early steps of alpha2beta1 integrin-mediated adhesion to collagen type I by using single-cell force spectroscopy. Mol Biol Cell 2007;18:1634-44. (Pubitemid 46717546)
17. Klemke M, Wabnitz GH, Funke F, Funk B, Kirchgessner H, Samstag Y. Oxidation of cofilin mediates T cell hyporesponsiveness under oxidative stress conditions. Immunity 2008;29:404-13.
18. Csoka M, Strauss G, Debatin KM, Moldenhauer G. Activation of T cell cytotoxicity against autologous common acute lymphoblastic leukemia (cALL) blasts by CD3xCD19 bispecific antibody. Leukemia 1996;10:1765-72. (Pubitemid 26380023)
19. Pfaff D, Heroult M, Riedel M, Reiss Y, Kirmse R, Ludwig T, Korff T, Hecker M, Augustin HG. Involvement of endothelial ephrin-B2 in adhesion and transmigration of EphB-receptor-expressing monocytes. J Cell Sci 2008;121:3842-50.
20. + CTL. Semin Immunol 2003;15:301-5.
21. Hosseini BH, Louban I, Djandji D, Wabnitz GH, Deeg J, Bulbuc N, Samstag Y, Gunzer M, Spatz JP, Hammerling GJ. Immune synapse formation determines interaction forces between T cells and antigen-presenting cells measured by atomic force microscopy. Proc Natl Acad Sci USA 2009;106:17852-7.
22. McGrath KE, Bushnell TP, Palis J. Multispectral imaging of hematopoietic cells: where flow meets morphology. J Immunol Methods 2008;336:91-7.
23. Ahmed F, Friend S, George TC, Barteneva N, Lieberman J. Numbers matter: quantitative and dynamic analysis of the formation of an immunological synapse using imaging flow cytometry. J Immunol Methods 2009;347:79-86.
24. Lub M, van Kooyk Y, Figdor CG. Ins and outs of LFA-1. Immunol Today 1995;16:479-83.
25. Konstandin MH, Sester U, Klemke M, Weschenfelder T, Wabnitz GH, Samstag Y. A novel flow-cytometry-based assay for quantification of affinity and avidity changes of integrins. J Immunol Methods 2006;310:67-77. (Pubitemid 43375977)
26. + T cells using atomic force microscopy. Biochem Biophys Res Commun 2008;374:90-4.
27. Shi X, Xu L, Yu J, Fang X. Study of inhibition effect of Herceptin on interaction between Heregulin and ErbB receptors HER3/HER2 by single-molecule force spectroscopy. Exp Cell Res 2009;315:2847-55.