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Volumn 705, Issue , 2011, Pages 87-107

Site-Specific Protein Labeling by Intein-Mediated Protein Ligation

Author keywords

expressed protein ligation; intein; Intein mediated protein ligation; protein labeling

Indexed keywords

FLUORESCENT DYE; HYBRID PROTEIN; INTEIN; SULFIDE;

EID: 79952456467     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61737-967-3_6     Document Type: Chapter
Times cited : (15)

References (22)
  • 1
    • 0005981053 scopus 로고    scopus 로고
    • Expression systems for production of heterologous proteins
    • Rai, M., Padh, H. (2001) Expression systems for production of heterologous proteins. Curr Sci 80, 1121–1128.
    • (2001) Curr Sci , vol.80 , pp. 1121-1128
    • Rai, M.1    Padh, H.2
  • 2
    • 0037208835 scopus 로고    scopus 로고
    • Purification of recombinant proteins from E. coli by engineered inteins
    • Xu, M. Q., Evans, T. C., Jr. (2003) Purification of recombinant proteins from E. coli by engineered inteins. Methods Mol Biol 205, 43–68.
    • (2003) Methods Mol Biol , vol.205 , pp. 43-68
    • Xu, M.Q.1    Evans, T.C.2
  • 4
    • 0344351815 scopus 로고    scopus 로고
    • Semisynthesis of cytotoxic proteins using a modified protein splicing element
    • Evans, T. C., Jr., Benner, J., Xu, M. Q. (1998) Semisynthesis of cytotoxic proteins using a modified protein splicing element. Protein Sci 7, 2256–2264.
    • (1998) Protein Sci , vol.7 , pp. 2256-2264
    • Evans, T.C.1    Benner, J.2    Xu, M.Q.3
  • 5
    • 0032499752 scopus 로고    scopus 로고
    • Expressed protein ligation: A general method for protein engineering
    • Muir, T. W., Sondhi, D., Cole, P. A. (1998) Expressed protein ligation: a general method for protein engineering. Proc Natl Acad Sci USA 95, 6705–6710.
    • (1998) Proc Natl Acad Sci USA , vol.95 , pp. 6705-6710
    • Muir, T.W.1    Sondhi, D.2    Cole, P.A.3
  • 6
    • 23444457741 scopus 로고    scopus 로고
    • Recent advances in protein splicing: Manipulating proteins in vitro and in vivo
    • Xu, M. Q., Evans, T. C., Jr. (2005) Recent advances in protein splicing: manipulating proteins in vitro and in vivo. Curr Opin Biotechnol 16, 440–446.
    • (2005) Curr Opin Biotechnol , vol.16 , pp. 440-446
    • Xu, M.Q.1    Evans, T.C.2
  • 7
    • 85139122864 scopus 로고    scopus 로고
    • Southworth, M. W., Amaya, K., Evans, T. C., Xu, M. Q., Perler, F. B. (1999) Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein. Biotechniques 27, 110–114, 116, 118–120.
    • Southworth, M. W., Amaya, K., Evans, T. C., Xu, M. Q., Perler, F. B. (1999) Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein. Biotechniques 27, 110–114, 116, 118–120.
    • (2001)
  • 8
    • 0033621158 scopus 로고    scopus 로고
    • Protein synthesis by native chemical ligation: Expanded scope by using straightforward methodology
    • Hackeng, T. M., Griffin, J. H., Dawson, P. E. (1999) Protein synthesis by native chemical ligation: expanded scope by using straightforward methodology. Proc Natl Acad Sci USA 96, 10068–10073.
    • (1999) Proc Natl Acad Sci USA , vol.96 , pp. 10068-10073
    • Hackeng, T.M.1    Griffin, J.H.2    Dawson, P.E.3
  • 9
    • 4444365081 scopus 로고    scopus 로고
    • Producing peptide arrays for epitope mapping by intein-mediated protein ligation
    • Sun, L., Rush, J., Ghosh, I., Maunus, J. R., Xu, M. Q. (2004) Producing peptide arrays for epitope mapping by intein-mediated protein ligation. Biotechniques 37, 430–436.
    • (2004) Biotechniques , vol.37 , pp. 430-436
    • Sun, L.1    Rush, J.2    Ghosh, I.3    Maunus, J.R.4    Xu, M.Q.5
  • 10
    • 33847695239 scopus 로고    scopus 로고
    • Use of intein-mediated phosphoprotein arrays to study substrate specificity of protein phos-phatases
    • Kochinyan, S., Sun, L., Ghosh, I., Barshevsky, T., Xu, J., Xu, M. Q. (2007) Use of intein-mediated phosphoprotein arrays to study substrate specificity of protein phos-phatases. Biotechniques 42, 63–69.
    • (2007) Biotechniques , vol.42 , pp. 63-69
    • Kochinyan, S.1    Sun, L.2    Ghosh, I.3    Barshevsky, T.4    Xu, J.5    Xu, M.Q.6
  • 11
    • 13444258243 scopus 로고    scopus 로고
    • Using protein-DNA chimeras to detect and count small numbers of molecules
    • Burbulis, I., Yamaguchi, K., Gordon, A., Carlson, R., Brent, R. (2005) Using protein-DNA chimeras to detect and count small numbers of molecules. Nat Methods 2, 31–37.
    • (2005) Nat Methods , vol.2 , pp. 31-37
    • Burbulis, I.1    Yamaguchi, K.2    Gordon, A.3    Carlson, R.4    Brent, R.5
  • 12
    • 0033575092 scopus 로고    scopus 로고
    • Biosynthesis of a head-to-tail cyclized protein with improved biological activity
    • Camarero, J. A., Muir, T. W. (1999) Biosynthesis of a head-to-tail cyclized protein with improved biological activity. JACS 121, 5597–5598.
    • (1999) JACS , vol.121 , pp. 5597-5598
    • Camarero, J.A.1    Muir, T.W.2
  • 13
    • 7944235893 scopus 로고    scopus 로고
    • An improved method for utilization of peptide substrates for antibody characterization and enzymatic assays
    • Ghosh, I., Sun, L., Evans, T. C., Jr., Xu, M. Q. (2004) An improved method for utilization of peptide substrates for antibody characterization and enzymatic assays. J Immunol Methods 293, 85–95.
    • (2004) J Immunol Methods , vol.293 , pp. 85-95
    • Ghosh, I.1    Sun, L.2    Evans, T.C.3    Xu, M.Q.4
  • 14
    • 18144374551 scopus 로고    scopus 로고
    • Site-specific immobilization of proteins in a microar-ray using intein-mediated protein splicing
    • Girish, A., Sun, H., Yeo, D. S., Chen, G. Y., Chua, T. K., Yao, S. Q. (2005) Site-specific immobilization of proteins in a microar-ray using intein-mediated protein splicing. Bioorg Med Chem Lett 15, 2447–2451.
    • (2005) Bioorg Med Chem Lett , vol.15 , pp. 2447-2451
    • Girish, A.1    Sun, H.2    Yeo, D.S.3    Chen, G.Y.4    Chua, T.K.5    Yao, S.Q.6
  • 15
    • 2942575037 scopus 로고    scopus 로고
    • Structure of the Rab7:REP-1 complex: Insights into the mechanism of Rab preny-lation and choroideremia disease
    • Rak, A., Pylypenko, O., Niculae, A., Pyatkov, K., Goody, R. S., Alexandrov, K. (2004) Structure of the Rab7:REP-1 complex: insights into the mechanism of Rab preny-lation and choroideremia disease. Cell 117, 749–760.
    • (2004) Cell , vol.117 , pp. 749-760
    • Rak, A.1    Pylypenko, O.2    Niculae, A.3    Pyatkov, K.4    Goody, R.S.5    Alexandrov, K.6
  • 16
    • 34250365042 scopus 로고    scopus 로고
    • Site-specific incorporation of flu-orotyrosines into the R2 subunit of E. coli ribonucleotide reductase by expressed protein ligation
    • Seyedsayamdost, M. R., Yee, C. S., Stubbe, J. (2007) Site-specific incorporation of flu-orotyrosines into the R2 subunit of E. coli ribonucleotide reductase by expressed protein ligation. Nat Protocol 2, 1225–1235.
    • (2007) Nat Protocol , vol.2 , pp. 1225-1235
    • Seyedsayamdost, M.R.1    Yee, C.S.2    Stubbe, J.3
  • 17
    • 36349027062 scopus 로고    scopus 로고
    • Improved segmental isotope labeling methods for the NMR study of multidomain or large proteins: Application to the RRMs of Npl3p and hnRNP L
    • Skrisovska, L., Allain, F. H. (2008) Improved segmental isotope labeling methods for the NMR study of multidomain or large proteins: application to the RRMs of Npl3p and hnRNP L. J Mol Biol 375, 151–164.
    • (2008) J Mol Biol , vol.375 , pp. 151-164
    • Skrisovska, L.1    Allain, F.H.2
  • 18
    • 34249020439 scopus 로고    scopus 로고
    • Design, preparation and use of ligated phosphopro-teins: A novel approach to study protein phos-phatases by dot blot array, ELISA and Western blot assays
    • Sun, L., Ghosh, I., Barshevsky, T., Kochinyan, S., Xu, M. Q. (2007) Design, preparation and use of ligated phosphopro-teins: a novel approach to study protein phos-phatases by dot blot array, ELISA and Western blot assays. Methods 42, 220–226.
    • (2007) Methods , vol.42 , pp. 220-226
    • Sun, L.1    Ghosh, I.2    Barshevsky, T.3    Kochinyan, S.4    Xu, M.Q.5
  • 19
    • 0242321289 scopus 로고    scopus 로고
    • Generation of an affinity column for antibody purification by intein-mediated protein ligation
    • Sun, L., Ghosh, I., Xu, M. Q. (2003) Generation of an affinity column for antibody purification by intein-mediated protein ligation. J Immunol Methods 282, 45–52.
    • (2003) J Immunol Methods , vol.282 , pp. 45-52
    • Sun, L.1    Ghosh, I.2    Xu, M.Q.3
  • 21
    • 0016748006 scopus 로고
    • Reduction of S-sulpho groups by tributylphos-phine: An improved method for the recombination of insulin chains
    • Ruegg, U. T., Gattner, H. G. (1975) Reduction of S-sulpho groups by tributylphos-phine: an improved method for the recombination of insulin chains. Hoppe Seylers Z Physiol Chem 356, 1527–1533.
    • (1975) Hoppe Seylers Z Physiol Chem , vol.356 , pp. 1527-1533
    • Ruegg, U.T.1    Gattner, H.G.2
  • 22
    • 2942568257 scopus 로고    scopus 로고
    • Western blot analysis of Src kinase assays using peptide substrates ligated to a carrier protein
    • Xu, J., Sun, L., Ghosh, I., Xu, M. Q. (2004) Western blot analysis of Src kinase assays using peptide substrates ligated to a carrier protein. Biotechniques 36, 976–978, 980–981.
    • (2004) Biotechniques , vol.36 , pp. 976-978
    • Xu, J.1    Sun, L.2    Ghosh, I.3    Xu, M.Q.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.