Light and electron microscopic study of the toxic effect of prolonged lead exposure on the seminiferous tubules of albino rats and the possible protective effect of ascorbic acid

Food and Chemical Toxicology

Volumn 49, Issue 4, 2011, Pages 734-743

  Shafai, Adel El ^a   Zohdy, Nahed ^a   Mulla, Khaled El ^a   Hassan, Manal ^a   Morad, Noha ^a  

^a ALEXANDRIA UNIVERSITY   (Egypt)

Author keywords

Ascorbic acid; Lead toxicity; Testis

Indexed keywords

ASCORBIC ACID; LEAD ACETATE;

ACROSOME; ANIMAL EXPERIMENT; ANIMAL TISSUE; APOPTOSIS; ARTICLE; BASEMENT MEMBRANE; CELL ULTRASTRUCTURE; CELL VACUOLE; CONTROLLED STUDY; CYTOPLASM; ELECTRON MICROSCOPY; HETEROCHROMATIN; LEAD BLOOD LEVEL; LEAD POISONING; LONG TERM EXPOSURE; MALE; MICROSCOPY; NONHUMAN; RAT; SEMINIFEROUS TUBULE; SERTOLI CELL; SPERMATID; SPERMATOCYTE; SPERMATOGONIUM; SPERMATOZOON ABNORMALITY; SPERMATOZOON TAIL;

ANIMALS; ASCORBIC ACID; LEAD; MALE; MICROSCOPY; MICROSCOPY, ELECTRON; RATS; RATS, WISTAR; SEMINIFEROUS TUBULES;

ANIMALIA; MYOIDA; RATTUS;

EID: 79952438783     PISSN: 02786915     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.fct.2010.11.033     Document Type: Article

References (49)

1. Acharya R.U., Acharya S., Mishra M. Lead acetate induced cytotoxicity in male germinal cells of swiss mice. Ind. Health 2003, 41:291-294.
2. Adhikari N., Sinha N., Narayan R., Saxena K.D. Lead-induced cell death in testes of young rats. J. Appl. Toxicol. 2001, 21:275-277.
3. Apostoli P., Boffetta P., Landrigan P. The reproductive toxicity and carcinogenicity of Lead: a critical review. Am. J. Ind. Med. 2000, 38:231-243.
4. Attri J., Dhawan V., Mahmoud S., Pandhi P., Parwana H.K., Nath R. Effect of vitamin C supplementation on oxidative DNA damage in an experimental model of lead-induced hypertension. Ann. Nutr. Metab. 2003, 47:294-301.
5. Aydos K., Guven C.M., Can B. Nicotine toxicity to the ultrastructure of the testis in rats. BJU International 2001, 88:622-626.
6. Balen H.A., Rutherford J.A. Management of infertility. BMJ 2007, 335(7620):608-711.
7. Benoff S., Jacob A., Hurley R.I. Male infertility and environmental exposure to lead and cadmium. Hum. Reprod. 2000, 6(2):107-121.
8. Bozzola J.J. Electron microscopy principles and techniques for biologists. Principles and Techniques, Specimen Preparation for Transmission EM 1999, 220-232. John and Berlett Publishers, Boston and London. second ed. J.J. Bozzola, L.D. Russel (Eds.).
9. Corpas I., Castillo M., Marquina D., Benito M. Lead Intoxication in Gestational and lactational periods alters the development of male reproductive organs. Ecotoxicol. Environ. Saf. 2002, 53:259-266.
10. Cotran R.S., Kumar V., Robbins S.L. Robbins Pathological Basis of Disease 2005, WB Saunders Company, Philadelphia, p. 1-34. seventh ed.
11. Creasy D.M. Pathogenesis of male reproductive toxicity. Toxicol. Pathol. 2001, 29(1):64-70.
12. Cross P.C. Cells and tissue ultrastructure: a functional perspective 1993, W.H. Freeman and Company, New York, p. 348. second ed.
13. Dhawan M., Kachru D.N., Tandon S.K. Influence of thiamin and ascorbic acid supplementation on the antidotal efficacy of thiol chelators in experimental lead intoxication. Arch. Toxicol. 1988, 62:301-304.
14. Drury R.A.B., Wallington E.A. Carlton's Histological Technique 1980, Oxford University Press, Oxford, p. 195-196. fifth ed.
15. Farrell S.E., Vandevander P., Schoffstall J.M., Lee D.C. Blood lead levels in emergency department patients with retained lead bullets and shrapnel. Acad. Emerg. Med. 1999, 6(3):208-212.
16. Gidlow D.A. Lead toxicity, in-depth review. J. occup. Med. 2004, 54:76-81.
17. Haider S.G., Passia D., Servos G., Hettwer J. Electron microscopic evidence for deep invaginations of the lamina propria towards the seminiferous tubule lumen in a patient with varicocele. Int. J. Androl. 1986, 9:27-32.
18. Henretig F.M. Lead. Gold Frank's Toxicological Emergencies 2002, 1200-1237. McGraw Hill Companies, New York. seventh ed. G. Lewis, N. Lewis, M.A. Howland, L. Nelson (Eds.).
19. Jequier A.M. Male Infertility - A Guide for the Clinician 2002, Blackwell Science, Oxford.
20. John H. The relevance of spontaneous and chemically induced alterations in testicular germ cell apoptosis to toxicology. Toxicol. Lett. 2000, 112-113:79-86.
21. Kumar S. Occupational exposure associated with reproductive dysfunction. J. Occup. Health 2004, 46:1-19.
22. Li G.X., Kang K.S., Lee Y.S. 2 Bromopeopane induced germ cell apoptosis during spermatogenesis in male rat. J. Vet. Med. Sci. 2001, 63(4):373-382.
23. Markowitz M. Lead poisoning: a disease for the next millennium. Curr. Probl. Pediatr. 2000, 30(3):62-70.
24. Meistrich M.I., Trostle-Weige P.K., Russell L.D. Abnormal manchette development in spermatids of mutant mice. Am. J. Anat. 1990, 188:64-74.
25. Murthy C., Saxena K.D., Gupta K.S., Chandra V.S. Lead induced ultrastructural changes in the testis of rats. Exp. Pathol. 1991, 42:95-100.
26. Oliveira M.A., Fortes Z.B. Ascorbic acid supplementation restores defective leukocyte- endothelial interaction in alloxan - diabetic rats. Diabetes Metab. Res. Rev. 2002, 19:60-68.
27. Pace M.B., Lawrence A.D., Behr J.M., Parson J.P., Dias A.J. Neonatal lead exposure changes quality of sperm and number of macrophages in testes of BALB/c mice. Toxicology 2005, 210:247-256.
28. Paniagua R., Martinez-Onsurbe P., Santamaria L., Nistal M., Praille B. Ultrastructural and quantitative study of the lamina propria in cryptorchid human testes. Int. J. Androl. 1990, 13:470-487.
29. Patra R., Swarup D. Effect of antioxidants ascorbic acid, l-methionine on tocopherol alone or along with chelators on cardiac tissue of lead treated rats. Vet. Arch. 2004, 74:235-244.
30. Perkin A. Perkin Elmer Corporation. Analytical methods for atomic absorption spectrophotometry 1984, Connecticut, Perkin Elmer, Norwalk, second ed.
31. Pirkle J.L., Kaufmann R.B., Brody D.J. Exposure of the U.S. population to lead, 1991-1994. Environ. Health Perspect. 1998, 106(11):745-750.
32. Queiroz E.K., Waissmann W. Occupational exposure and effects on the male reproductive system.Cad Saùde Pùblica. Rio de Janeiro 2006, 22(3):485-493.
33. Ramanathan K., Balakumar B., Panneerselvam C. Effects of ascorbic acid and alpha tocopherol on arsenic induced oxidative stress. Hum. Exp. Toxicol. 2002, 21:675-680.
34. Reid L.M., Jefferson D. Cell culture studies using extracts of extracellular matrix to study growth and differentiation in mammalian cells. Mammalian Cell Culture 1984, Plenum Publishing Corp, New York. J.P. Mather (Ed.).
35. Russel L.D., Ying L., Overbeek P.A. Insertional mutation that causes acrosomal hypodevelopment: its relationship to sperm head shaping. Anat. Rec. 1994, 238:437-453.
36. Saleh R.A., Agarwal A., Nada E.A., El Tonsy M.H., Sharma R.K., Meyer A., Nelson D.R., Thomas A.J. Negative effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility. Fertil. Steril. 2003, 79(30):1597-1605.
37. Sanocka D., Kurpisz M. Reactive oxygen species and sperm cells: a review. Reprod. Biol. Endocrinol. 2004, 2(12):1-7.
38. Schwemberger J.G., Mosby J.E., Doa M.J. Blood Lead Levels- United States, 1999-2002. MMWR 2005 1999, 54(20):513-516.
39. Shalan M.A., Fortes Z.B., Mostafa M.S., Hassouna M.M., El Nabi S.E., El Refaie A. Amelioration of lead toxicity on rat liver with vitamin C and silymarin supplements. Toxicology. 2005, 206:1-15.
40. Talisman S., Cvitkovic P., Jurasovic J., Pizent A., Gavella M., Rocic B. Semen quality and reproductive endocrine function in relation to biomarkers of lead, cadmium, zinc, and copper in men. Environ. Health Prespect. 2000, 108:45-53.
41. Tandon K.S., Singh S. Role of vitamins in treatment of lead intoxication. J. Trace Elem. Exp. Med. 2000, 13:305-315.
42. Tandon S.K., Chatteriee M., Bhargava A., Shukla V., Bihari V. Lead poisoning in Indian silver refiners. Sci. Total Environ. 2001, 17:177-182.
43. Trevor G., Graham R. Electron microscopy2: practical procedures. Theory and Practice of Histological Techniques 1996, Churchill Livingstone, New York, Edinburgh, London, Madrid, Melbourne, San Francisco and Tokyo. fourth ed. J.D. Bancroft, A. Stevens (Eds.).
44. Vaziri N.D., Sica D.A. Lead induced hypertension: role of oxidative stress. Curr. Hypertens. Rep. 2004, 6:314-320.
45. Volko M., Morris H., Cronin M.H. Review metals, toxicity and oxidative stress. Curr. Med. Chem. 2005, 12:1161-1208.
46. Watson W.A., Litovitz T.L., Klein-Schwartz W.I. 2003 Annual report of the American association of poison control centers toxic exposure surveillance system. Am. J. Emerg. Med. 2004, 22(5):335-404.
47. Wong W.Y., Thomas C.M.G., Merkus J.M., Zielhuis G.A., Steegers R.P. Male factor subfertility: possible causes and the impact of nutritional factors. Fertil. Steril. 2000, 73:435-442.
48. Ye X.B., Fu H., Zhu J.L., Ni W.M., Lu Y.W. A study on oxidative stress in lead-exposed workers. J. Toxicol. Health. 1999, 57:161-172.
49. Zamboni L. Sperm structure and its relevance to infertility: an electron microscopic study. Arch. Pathol. Lab. Med. 1992, 116:325.