Process optimization for recombinant protein production in the psychrophilic bacterium Pseudoalteromonas haloplanktis

Process Biochemistry

Volumn 46, Issue 4, 2011, Pages 953-959

  Giuliani, Maria ^a   Parrilli, Ermenegilda ^a   Ferrer, Pau ^b   Baumann, Kristin ^b   Marino, Gennaro ^a   Tutino, Maria Luisa ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^b UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

3H6 Fab; Antibody fragment; Psychrophiles; Recombinant protein production; Synthetic medium

Indexed keywords

3H6 FAB; ANTIBODY FRAGMENT; PSYCHROPHILES; RECOMBINANT PROTEIN PRODUCTIONS; SYNTHETIC MEDIUM;

AMINO ACIDS; ANTIBODIES; BACTERIA; CELL CULTURE;

OPTIMIZATION;

BACTERIA (MICROORGANISMS); PSEUDOALTEROMONAS; PSEUDOALTEROMONAS HALOPLANKTIS;

EID: 79952312611     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2011.01.011     Document Type: Article

References (25)

1. N. Ferrer-Miralles, J. Domingo-Espín, J.L. Corchero, E. Vázquez, and A. Villaverde Microbial factories for recombinant pharmaceuticals Microb Cell Fact 8 2009 17
2. L.F. Vallejo, and U. Rinas Strategy for recovery of active protein through refolding of bacterial inclusion body proteins Microb Cell Fact 3 2004 2 12
3. F. Baneyx, and M. Mujacic Recombinant protein folding and misfolding in Escherichia coli Nat Biotechnol 22 11 2004 1399 1408
4. E. Parrilli, A. Duilio, and M.L. Tutino Heterologous protein expression in psychrophilic hosts R. Margesin, F. Schinner, J.C. Marx, C. Gerday, Psychrophiles: from biodiversity to biotechnology 2008 Springer-Verlag Berlin Heidelberg 365 379
5. C. Médigue, E. Krin, G. Pascal, V. Barbe, A. Bernsel, P.N. Bertin, F. Cheung, S. Cruveiller, S. D'Amico, A. Duilio, G. Fang, G. Feller, C. Ho, S. Mangenot, G. Marino, J. Nilsson, E. Parrilli, E.P. Rocha, Z. Rouy, A. Sekowska, M.L. Tutino, D. Vallenet, G. von Heijne, and A. Danchin Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125 Genome Res 15 10 2005 1325 1335 (Pubitemid 41400898)
6. R. Papa, S. Glagla, A. Danchin, T. Schweder, G. Marino, and A. Duilio Proteomic identification of a two-component regulatory system in Pseudoalteromonas haloplanktis TAC125 Extremophiles 10 6 2006 483 491 (Pubitemid 44764306)
7. R. Papa, V. Rippa, G. Sannia, G. Marino, and A. Duilio An effective cold inducible expression system developed in Pseudoalteromonas haloplanktis TAC125 J Biotechnol 127 2 2007 199 210 (Pubitemid 46107135)
8. D. Hanahan Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166 1983 557 580 (Pubitemid 13027898)
9. A. Duilio, S. Madonna, M.L. Tutino, M. Pirozzi, G. Sannia, and G. Marino Promoters from a cold-adapted bacterium: definition of a consensus motif and molecular characterization of UP regulative elements Extremophiles 8 2004 125 132 (Pubitemid 40876335)
10. J. Sambrook, D.W. Russell, Molecular cloning in a laboratory manual. 3 2001 Cold Spring Harbor Laboratory Cold Spring Harbor, NY
11. R.E. Kunert, R. Weik, B. Ferko, G. Stiegler, and H. Katinger Anti-idiotypic antibody Ab2/3H6 mimics the epitope of the neutralizing anti-HIV-1 monoclonal antibody 2F5 AIDS 16 4 2002 667 668 (Pubitemid 34218864)
12. J.S. Gach, M. Maurer, R. Hahna, B. Gasser, D. Mattanovich, H. Katinger, and R. Kunert High level expression of a promising anti-idiotypic antibody fragment vaccine against HIV-1 in Pichia pastoris J Biotechnol 128 2007 735 746 (Pubitemid 46341491)
13. I. Vigentini, A. Merico, M.L. Tutino, C. Compagno, and G. Marino Optimization of recombinant human nerve growth factor production in the psychrophilic Pseudoalteromonas haloplanktis J Biotechnol 127 2006 141 150 (Pubitemid 46107122)
14. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 1-2 1976 248 254
15. A. Hoyoux, I. Jennes, P. Dubois, S. Genicot, F. Dubail, J.M. Francois, E. Baise, G. Feller, and C. Gerday Cold adapted beta-galactosidase from the Antarctic psychrophile Pseudoalteromonas haloplanktis Appl Environ Microbiol 67 2001 1529 1535 (Pubitemid 33644919)
16. D.L. Nelson, and M.M. Cox Lehninger principles of biochemistry, W.H. Freeman 5th ed. 2008
17. D.L. Oxender, and S.C. Quay Regulation of leucine transport and binding proteins in Escherichia coli J Cell Physiol 89 4 1976 517 521
18. W.R. Penrose, G.E. Nichoalds, J.R. Piperino, and D.L. Oxender Purification and properties of a leucine-binding protein from Escherichia coli J Biol Chem 243 22 1968 5921 5928
19. S.C. Quay, and D.L. Oxender Regulation of branched-chain amino acid transport in Escherichia coli J Bacteriol 127 3 1976 1225 1238
20. M. Rahmanian, D.R. Claus, and D.L. Oxender Multiplicity of leucine transport systems in Escherichia coli K-12 J Bacteriol 116 3 1973 1258 1266
21. S.A. Haney, J.V. Platko, D.L. Oxender, and J.M. Calvo Lrp, a leucine-responsive protein, regulates branched-chain amino acid transport genes in Escherichia coli J Bacteriol 174 1 1992 108 115
22. J.R. Landgraf, J.A. Boxer, and J.M. Calvo Escherichia coli Lrp (leucine-responsive regulatory protein) does not directly regulate expression of the leu operon promoter J Bacteriol 181 20 1999 6547 6551 (Pubitemid 29512970)
23. S.C. Quay, T.E. Dick, and D.L. Oxender Role of transport systems in amino acid metabolism: leucine toxicity and the branched-chain amino acid transport systems J Bacteriol 129 3 1977 1257 1265 (Pubitemid 8109663)
24. B. Gasser, M. Maurer, J. Rautio, M. Sauer, A. Bhattacharyya, M. Saloheimo, M. Penttilä, and D. Mattanovich Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions BMC Genomics 8 2007 179
25. Dragosits M, Frascotti G, Bernard-Granger L, Vázquez F, Giuliani M, Baumann K, et al. Influence of growth temperature on the production of antibody Fab fragments in different microbes: A host comparative analysis. Biotechnol Progress, doi:10.1002/btpr.524.