Impact of blood processing variations on natural killer cell frequency, activation, chemokine receptor expression and function

Journal of Immunological Methods

Volumn 366, Issue 1-2, 2011, Pages 28-35

  Naranbhai, Vivek ^a   Bartman, Pat ^b   Ndlovu, Dudu ^b   Ramkalawon, Pamela ^a   Ndung'u, Thumbi ^a   Wilson, Douglas ^b   Altfeld, Marcus ^a,c   Carr, William H ^a,c  

^a UNIVERSITY OF KWAZULU NATAL   (South Africa)

^b Edendale Regional Hospital   (South Africa)

^c HARVARD UNIVERSITY   (United States)

Author keywords

Activation; Chemokine receptor; Flow cytometry; Natural killer cells; PBMC; Whole blood

Indexed keywords

CHEMOKINE RECEPTOR CCR4; CHEMOKINE RECEPTOR CCR7; GAMMA INTERFERON; LYSOSOME ASSOCIATED MEMBRANE PROTEIN 1; TUMOR NECROSIS FACTOR ALPHA;

ADULT; ARTICLE; BLOOD; BLOOD SAMPLING; CELL ACTIVATION; DENSITY GRADIENT CENTRIFUGATION; FEMALE; FLOW CYTOMETRY; HUMAN; IMMUNE RESPONSE; INNATE IMMUNITY; NATURAL KILLER CELL; NORMAL HUMAN; PATHOGENESIS; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; VEIN PUNCTURE;

ADULT; BLOOD SPECIMEN COLLECTION; CELL DEGRANULATION; CELL SEPARATION; CENTRIFUGATION, DENSITY GRADIENT; FEMALE; FLOW CYTOMETRY; HUMANS; KILLER CELLS, NATURAL; LEUKOCYTES, MONONUCLEAR; LYMPHOCYTE ACTIVATION; PHLEBOTOMY; RECEPTORS, CHEMOKINE; TIME FACTORS; YOUNG ADULT;

EID: 79952311578     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2011.01.001     Document Type: Article

References (25)

1. Alter G., Malenfant J.M., Altfeld M. CD107a as a functional marker for the identification of natural killer cell activity. J. Immunol. Meth. 2004, 294:15.
2. Berahovich R.D., Lai N.L., Wei Z., Lanier L.L., Schall T.J. Evidence for NK cell subsets based on chemokine receptor expression. J. Immunol. 2006, 177:7833.
3. Berhanu D., Mortari F., De Rosa S.C., Roederer M. Optimized lymphocyte isolation methods for analysis of chemokine receptor expression. J. Immunol. Meth. 2003, 279:199.
4. Betensky R.A., Connick E., Devers J., Landay A.L., Nokta M., Plaeger S., Rosenblatt H., Schmitz J.L., Valentine F., Wara D., Weinberg A., Lederman H.M. Shipment impairs lymphocyte proliferative responses to microbial antigens. Clin. Diagn. Lab. Immunol. 2000, 7:759.
5. Bull M., Lee D., Stucky J., Chiu Y.L., Rubin A., Horton H., McElrath M.J. Defining blood processing parameters for optimal detection of cryopreserved antigen-specific responses for HIV vaccine trials. J. Immunol. Meth. 2007, 322:57.
6. De Maria A., Fogli M., Costa P., Murdaca G., Puppo F., Mavilio D., Moretta A., Moretta L. The impaired NK cell cytolytic function in viremic HIV-1 infection is associated with a reduced surface expression of natural cytotoxicity receptors (NKp46, NKp30 and NKp44). Eur. J. Immunol. 2003, 33:2410.
7. Ekong T., Kupek E., Hill A., Clark C., Davies A., Pinching A. Technical influences on immunophenotyping by flow cytometry. The effect of time and temperature of storage on the viability of lymphocyte subsets. J. Immunol. Meth. 1993, 164:263.
8. Fogli M., Costa P., Murdaca G., Setti M., Mingari M.C., Moretta L., Moretta A., De Maria A. Significant NK cell activation associated with decreased cytolytic function in peripheral blood of HIV-1-infected patients. Eur. J. Immunol. 2004, 34:2313.
9. Fogli M., Mavilio D., Brunetta E., Varchetta S., Ata K., Roby G., Kovacs C., Follmann D., Pende D., Ward J., Barker E., Marcenaro E., Moretta A., Fauci A.S. Lysis of endogenously infected CD4+ T cell blasts by rIL-2 activated autologous natural killer cells from HIV-infected viremic individuals. PLoS Pathog. 2008, 4:e1000101.
10. Horowitz A., Behrens R.H., Okell L., Fooks A.R., Riley E.M. NK cells as effectors of acquired immune responses: effector CD4(+) T cell-dependent activation of NK cells following vaccination. J. Immunol. 2010, 185:2808.
11. Kierstead L.S., Dubey S., Meyer B., Tobery T.W., Mogg R., Fernandez V.R., Long R., Guan L., Gaunt C., Collins K., Sykes K.J., Mehrotra D.V., Chirmule N., Shiver J.W., Casimiro D.R. Enhanced rates and magnitude of immune responses detected against an HIV vaccine: effect of using an optimized process for isolating PBMC. AIDS Res. Hum. Retroviruses 2007, 23:86.
12. Lin S.J., Chao H.C., Yan D.C., Huang Y.J. Expression of adhesion molecules on T lymphocytes in young children and infants-a comparative study using whole blood lysis or density gradient separation. Clin. Lab. Haematol. 2002, 24:353.
13. McKenna K.C., Beatty K.M., Vicetti Miguel R., Bilonick R.A. Delayed processing of blood increases the frequency of activated CD11b+CD15+ granulocytes which inhibit T cell function. J. Immunol. Meth. 2009, 341:68.
14. Meier A., Fisher A., Sidhu H.K., Chang J.J., Wen T.F., Streeck H., Alter G., Silvestri G., Altfeld M. Rapid loss of dendritic cell and monocyte responses to TLR ligands following venipuncture. J. Immunol. Meth. 2008, 339:132.
15. Neel N.F., Schutyser E., Sai J., Fan G.H., Richmond A. Chemokine receptor internalization and intracellular trafficking. Cytokine Growth Factor Rev. 2005, 16:637.
16. Nicholson J.K., Jones B.M., Cross G.D., McDougal J.S. Comparison of T and B cell analyses on fresh and aged blood. J. Immunol. Meth. 1984, 73:29.
17. Orange J.S., Ballas Z.K. Natural killer cells in human health and disease. Clin. Immunol. 2006, 118:1.
18. Ravet S., Scott-Algara D., Bonnet E., Tran H.K., Tran T., Nguyen N., Truong L.X., Theodorou I., Barre-Sinoussi F., Pancino G., Paul P. Distinctive NK-cell receptor repertoires sustain high-level constitutive NK-cell activation in HIV-exposed uninfected individuals. Blood 2007, 109:4296.
19. Renzi P., Ginns L.C. Analysis of T cell subsets in normal adults. Comparison of whole blood lysis technique to Ficoll-Hypaque separation by flow cytometry. J. Immunol. Meth. 1987, 98:53.
20. Tamul K.R., Schmitz J.L., Kane K., Folds J.D. Comparison of the effects of Ficoll-Hypaque separation and whole blood lysis on results of immunophenotypic analysis of blood and bone marrow samples from patients with hematologic malignancies. Clin. Diagn. Lab. Immunol. 1995, 2:337.
21. Tiemessen C.T., Shalekoff S., Meddows-Taylor S., Schramm D.B., Papathanasopoulos M.A., Gray G.E., Sherman G.G., Coovadia A.H., Kuhn L. Cutting edge: unusual NK cell responses to HIV-1 peptides are associated with protection against maternal-infant transmission of HIV-1. J. Immunol. 2009, 182:5914.
22. Weinberg A., Song L.Y., Wilkening C., Sevin A., Blais B., Louzao R., Stein D., Defechereux P., Durand D., Riedel E., Raftery N., Jesser R., Brown B., Keller M.F., Dickover R., McFarland E., Fenton T. Optimization and limitations of use of cryopreserved peripheral blood mononuclear cells for functional and phenotypic T-cell characterization. Clin. Vaccine Immunol. 2009, 16:1176.
23. Wong, A.H., Williams, K., Reddy, S., Wilson, D., Giddy, J., Alter, G., Ghebremichael, M., Carrington, M.N., Ndung'u, T., Walker, B.D., Altfeld, M., Carr, W.H., 2010.
24. Zhang Y., Wallace D.L., de Lara C.M., Ghattas H., Asquith B., Worth A., Griffin G.E., Taylor G.P., Tough D.F., Beverley P.C., Macallan D.C. In vivo kinetics of human natural killer cells: the effects of ageing and acute and chronic viral infection. Immunology 2007, 121:258.
25. Ziegler S.F., Ramsdell F., Hjerrild K.A., Armitage R.J., Grabstein K.H., Hennen K.B., Farrah T., Fanslow W.C., Shevach E.M., Alderson M.R. Molecular characterization of the early activation antigen CD69: a type II membrane glycoprotein related to a family of natural killer cell activation antigens. Eur. J. Immunol. 1993, 23:1643.