Optimized lymphocyte isolation methods for analysis of chemokine receptor expression

Journal of Immunological Methods

Volumn 279, Issue 1-2, 2003, Pages 199-207

  Berhanu, Della ^a   Mortari, Frank ^b   De Rosa, Stephen C ^a   Roederer, Mario ^a  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

^b R and D Systems Inc   (United States)

Author keywords

Cell isolation; Chemokine receptors; Ficoll; Flow cytometry; Human; Immunophenotyping; Memory and naive T cells; PBMC; T lymphocytes; Whole blood

Indexed keywords

CHEMOKINE RECEPTOR;

8 COLOR FLOW CYTOMETRY; ARTICLE; BLOOD CELL; CELL ISOLATION; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; HUMAN; LYMPHOCYTE; MEMORY CELL; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEPARATION TECHNIQUE; STAINING; STANDARD;

EID: 0142092623     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(03)00186-8     Document Type: Article

References (13)

1. Berger E.A., Murphy P.M., Farber J.M. Chemokine receptors as HIV-1 coreceptors: roles in viral entry, tropism, and disease. Annu. Rev. Immunol. 17:1999;657-700.
2. Bruhl H., Wagner K., Kellner H., Schattenkirchner M., Schlondorff D., Mack M. Surface expression of CC- and CXC-chemokine receptors on leucocyte subsets in inflammatory joint diseases. Clin. Exp. Immunol. 126:2001;551-559.
3. Davenport M.P., Zaunders J.J., Hazenberg M.D., Schuitemaker H., van Rij R.P. Cell turnover and cell tropism in HIV-1 infection. Trends Microbiol. 10:2002;275-278.
4. Glatzel A., Wesch D., Schiemann F., Brandt E., Janssen O., Kabelitz D. Patterns of chemokine receptor expression on peripheral blood gamma delta T lymphocytes: strong expression of CCR5 is a selective feature of V delta 2/V gamma 9 gamma delta T cells. J. Immunol. 168:2002;4920-4929.
5. Horuk R. Chemokine receptors. Cytokine Growth Factor Rev. 12:2001;313-335.
6. Kantor A., Roederer M. FACS analysis of lymphocytes. 5th ed. Herzenberg L.A., Weir D.M., Herzenberg L.A., Blackwell C. Handbook of Experimental Immunology. vol. 2:1997;49.1-49.13 Blackwell Science, Cambridge.
7. Liao F., Rabin R.L., Smith C.S., Sharma G., Nutman T.B., Farber J.M. CC-chemokine receptor 6 is expressed on diverse memory subsets of T cells and determines responsiveness to macrophage inflammatory protein 3 alpha. J. Immunol. 162:1999;186-194.
8. Murdoch C., Finn A. Chemokine receptors and their role in inflammation and infectious diseases. Blood. 95:2000;3032-3043.
9. Nicholson J.K., Browning S.W., Hengel R.L., Lew E., Gallagher L.E., Rimland D., McDougal J.S. CCR5 and CXCR4 expression on memory and naive T cells in HIV-1 infection and response to highly active antiretroviral therapy. J. Acquir. Immune Defic. Syndr. 27:2001;105-115.
10. Roederer, M. http://www.drmr.com/abcon/index.html .
11. Shalekoff S., Tiemessen C.T. Duration of sample storage dramatically alters expression of the human immunodeficiency virus coreceptors CXCR4 and CCR5. Clin. Diagn. Lab. Immunol. 8:2001;432-436.
12. Wang J.M., Deng X., Gong W., Su S. Chemokines and their role in tumor growth and metastasis. J. Immunol. Methods. 220:1998;1-17.
13. Zlotnik A., Morales J., Hedrick J.A. Recent advances in chemokines and chemokine receptors. Crit. Rev. Immunol. 19:1999;1-47.