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Volumn 114, Issue 45, 2010, Pages 14487-14494

Photo- And biophysical studies of lectin-conjugated fluorescent nanoparticles: Reduced sensitivity in high density assays

Author keywords

[No Author keywords available]

Indexed keywords

CARBOHYDRATES; CARBOXYLIC ACIDS; FLUORESCENCE; LUMINANCE; MICROARRAYS; PROBES; PROTEINS; SILICA; TISSUE;

EID: 79952279041     PISSN: 15206106     EISSN: 15205207     Source Type: Journal    
DOI: 10.1021/jp101854m     Document Type: Article
Times cited : (12)

References (83)
  • 40
    • 84906368998 scopus 로고    scopus 로고
    • To facilitate comparisons of these ConA conjugate solutions, Table S3 in the Supporting Information lists the ConA content, particle number density, and dye molecule density for each of the four solutions used to incubate carbohydrate microarrays
    • To facilitate comparisons of these ConA conjugate solutions, Table S3 in the Supporting Information lists the ConA content, particle number density, and dye molecule density for each of the four solutions used to incubate carbohydrate microarrays.
  • 43
    • 84906397598 scopus 로고    scopus 로고
    • The Bauhinia purpurea lectin, BP, is a Gal-β-l,3-GalNAc binder with a broad specificity, The data from BP lectin demonstrates that the fluorescence saturation observed from ConA at mannose targets is produced by another lectin at its preferred carbohydrate targets
    • The Bauhinia purpurea lectin, BP, is a Gal-β-l,3-GalNAc binder with a broad specificity, (www.eylabs.com). The data from BP lectin demonstrates that the fluorescence saturation observed from ConA at mannose targets is produced by another lectin at its preferred carbohydrate targets.
  • 44
    • 84906383334 scopus 로고    scopus 로고
    • c(Lectin × N)}
    • c([Lectin × N)}.
  • 45
    • 84906383335 scopus 로고    scopus 로고
    • note
    • 18e yielding an emission detection ratio of 0.8. The product of the ratios of extinction coefficient, quantum yield, and detection yields limits of 2.6-3.7.
  • 46
    • 84906397594 scopus 로고    scopus 로고
    • Promega Website
    • Promega Website, http:// www.promega.com/geneticidproc/ussymp8proc/21. html (2010).
    • (2010)
  • 49
    • 84906397595 scopus 로고    scopus 로고
    • Invitrogen Website, (2010)
    • Invitrogen Website, http:// www.invitrogen.com/site/us/en/home/support/ Research-Tools/FluorescenceSpectraViewer.html (2010).
  • 50
    • 84906397596 scopus 로고    scopus 로고
    • In PBS (pH 7.3), DLS values of the f(FL)NP diameters were 124 (FL-APTS added in ethanol) and 80 nm (FL-APTS added in THF)
    • In PBS (pH 7.3), DLS values of the f(FL)NP diameters were 124 (FL-APTS added in ethanol) and 80 nm (FL-APTS added in THF).
  • 51
    • 84906397591 scopus 로고    scopus 로고
    • The percentage of fNP that passed through 0.10 μm Anatop filters was 95-100% for FL-APTS addition in THF and 70-80% for FL-APTS addition in ethanol
    • The percentage of fNP that passed through 0.10 μm Anatop filters was 95-100% for FL-APTS addition in THF and 70-80% for FL-APTS addition in ethanol.
  • 54
    • 84906397592 scopus 로고    scopus 로고
    • 22b,c The absorbances of the resulting solutions differed by less than 5%
    • 22b,c The absorbances of the resulting solutions differed by less than 5%.
  • 57
    • 84906368997 scopus 로고    scopus 로고
    • 23b reported that the time interval between dye addition and the initiation of NP growth influences the amount of dye incorporated and the fluorescence efficiency
    • 23b reported that the time interval between dye addition and the initiation of NP growth influences the amount of dye incorporated and the fluorescence efficiency,
  • 59
    • 84906383332 scopus 로고    scopus 로고
    • (a) For 1 × additions, the FL concentration inside of a NP is 3-4 mM; 15-20% of the FL-APTS added to the inverse micelle solution is cross-linked into the NP silica matrix
    • (a) For 1 × additions, the FL concentration inside of a NP is 3-4 mM; 15-20% of the FL-APTS added to the inverse micelle solution is cross-linked into the NP silica matrix,
  • 60
    • 84906397593 scopus 로고    scopus 로고
    • For 3× additions in EtOH, the FL concentration inside of a NP is [FL]-NP = 12-15 mM (24-28% incorporation of FL-APTS). For 5× additions in EtOH, it is [FL]-NP = 25-28 mM (28-31% incorporation). For 8× additions, it is [FL]-NP = 38-40 mM (27-28% incorporation)
    • For 3× additions in EtOH, the FL concentration inside of a NP is [FL]-NP = 12-15 mM (24-28% incorporation of FL-APTS). For 5× additions in EtOH, it is [FL]-NP = 25-28 mM (28-31% incorporation). For 8× additions, it is [FL]-NP = 38-40 mM (27-28% incorporation).
  • 61
    • 84906383333 scopus 로고    scopus 로고
    • The difference of FL incorporation for 8× FL-APTS addition at 0 and 8 hr (28%) is twice as large as the standard deviation of FL incorporation in 8× 0 hr samples (15%)
    • The difference of FL incorporation for 8× FL-APTS addition at 0 and 8 hr (28%) is twice as large as the standard deviation of FL incorporation in 8× 0 hr samples (15%).
  • 62
    • 84906412001 scopus 로고    scopus 로고
    • 26b.c
    • 26b.c,
  • 65
    • 84906412002 scopus 로고    scopus 로고
    • 2 of surface
    • 2 of surface.
  • 67
    • 84906397588 scopus 로고    scopus 로고
    • 29d than the ConA tetramer. Thus, the ConA tetramer was used for this intensity calculation, Jmol: an opensource Java viewer for chemical structures in 3D
    • 29d than the ConA tetramer. Thus, the ConA tetramer was used for this intensity calculation, Jmol: an opensource Java viewer for chemical structures in 3D. http://www.jmol.org/.
  • 73
    • 84906368996 scopus 로고    scopus 로고
    • 29a
    • 29a
  • 78
    • 84906397589 scopus 로고    scopus 로고
    • note
    • 2) reacted with α-Dmannopyranosylphenyl isothiocyanate (M-beads) were incubated with ConA-f(TR)NP solution for 30 min. The beads were washed three times with PBS containing 0.05% Tween20. Fluorescence intensity conferred by ConA-fNP binding to the M-beads was quantified using a Zeiss Axiovert 200M fluorescence microscope. The binding between ConA-fNP and M-beads could be inhibited by preincubating ConA-fNP with 100 mM methyl-α-D-mannopyranoside for 30 min before incubating with M-beads. This preincubation of freshly prepared ConA-fNP reduced the M-bead fluorescence signal by 35%. After storing ConA-fNP for 12 days, preincubation with 100 mM free mannnoside reduced M-bead fluorescence by only 3%.
  • 79
    • 84906397590 scopus 로고    scopus 로고
    • note
    • -1 (MW (tetramer) = 1.06 × 105 g/mol). (b) Concanavalin A Product Information Sheet - Sigma Prod. No. C2010. CD Values used to estimate ConA loading per mg of fNP CD/mdeg 196 nm maximum 223 nm minimum Native ConA 43 8.98 -4.28 μg/mL ConA fNP 0.85 mg/mL 7.52 →42 μg -4.59 →54 μg of ConA/mg of fNP of ConA/mg of fNP
  • 80
    • 84906411998 scopus 로고    scopus 로고
    • 21 is 97attograms. This loading corresponds to 250 μg of ConA/mg of ConA-fNP
    • 21 is 97attograms. This loading corresponds to 250 μg of ConA/mg of ConA-fNP.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.