Fluorescence from airborne microparticles: Dependence on size, concentration of fluorophores, and illumination intensity

Applied Optics

Volumn 40, Issue 18, 2001, Pages 3005-3013

  Hill, Steven C ^a   Pinnick, Ronald G ^a   Niles, Stanley ^a   Fell, Nicholas F ^a   Pan, Yong Le ^b   Bottiger, Jerold ^c   Bronk, Burt V ^d   Holler, Stephen ^e   Chang, Richard K ^e  

^a U S ARMY RESEARCH LABORATORY   (United States)

^b NEW MEXICO STATE UNIVERSITY   (United States)

^c US Army Soldier Biol Chem C   (United States)

^d EDGEWOOD CHEMICAL BIOLOGICAL CENTER   (United States)

^e YALE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; LASER BEAMS; LIGHTING; QUENCHING; RAMAN SCATTERING;

FLUOROPHORES;

FLUORESCENCE;

EID: 0002988744     PISSN: 1559128X     EISSN: 21553165     Source Type: Journal    
DOI: 10.1364/AO.40.003005     Document Type: Article

References (54)

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50. 46 A more accurate model might include a calculation of the contribution to the imaginary part of the refractive index [as in Eq. (1)] from each species of fluorophore in the particle. This would also account more rigorously for energy transfer between molecules; however, that is beyond the scope of this paper and probably beyond the accuracy of our measurements. The shape of the calculated curve in Fig. 3(a) is somewhat sensitive to the concentration of tryptophan. However, our data do not appear accurate enough to distinguish between 2% and 4% tryptophan. A further limitation is that the B. sub-tilis was used as purchased, and we do not know the purity of the sample.
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